(Linear) (Six-base) MAPSORT of: KIBB8285.seq  Check: 7753  from: 1  to: 1182



KIBB8285.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     780    1182 
   Size:        470     310     402

  Fragments arranged by size: 

                 470     402     310
 
AleI CACnn'nnGTG

Cuts at:      0     373     443     493    1182 
   Size:        373      70      50     689

  Fragments arranged by size: 

                 689     373      70      50
 
ApoI r'AATT_y

Cuts at:      0     649     940     958    1182 
   Size:        649     291      18     224

  Fragments arranged by size: 

                 649     291     224      18
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      82     115    1182 
   Size:         82      33    1067

  Fragments arranged by size: 

                1067      82      33
 
BbvCI CC'TCA_GC

Cuts at:      0      38    1182 
   Size:         38    1144
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     945    1182 
   Size:        945     237
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1057    1182 
   Size:       1057     125
 
Bpu10I CC'TnA_GC

Cuts at:      0      38     120     389     693    1182 
   Size:         38      82     269     304     489

  Fragments arranged by size: 

                 489     304     269      82      38
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283    1182 
   Size:        283     899
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     740    1182 
   Size:        740     442
 
BsaAI yAC'GTr

Cuts at:      0     787    1182 
   Size:        787     395
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1060    1182 
   Size:       1060     122
 
BseYI C'CCAG_C

Cuts at:      0    1174    1182 
   Size:       1174       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     519    1182 
   Size:        519     663
 
BsmI GAATG_Cn'

Cuts at:      0    1143    1182 
   Size:       1143      39
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1138    1182 
   Size:       1138      44
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     168     343    1182 
   Size:        168     175     839

  Fragments arranged by size: 

                 839     175     168
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      63     336    1182 
   Size:         63     273     846

  Fragments arranged by size: 

                 846     273      63
 
ClaI AT'CG_AT

Cuts at:      0     584    1182 
   Size:        584     598
 
DraI TTT'AAA

Cuts at:      0     957    1182 
   Size:        957     225
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     331    1182 
   Size:        331     851
 
EarI CTCTTCn'nnn_

Cuts at:      0     271    1182 
   Size:        271     911
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     780    1057    1182 
   Size:        470     310     277     125

  Fragments arranged by size: 

                 470     310     277     125
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42     395    1065    1182 
   Size:         42     353     670     117

  Fragments arranged by size: 

                 670     353     117      42
 
MslI CAynn'nnrTG

Cuts at:      0     166     341     373     443     493    1182 
   Size:        166     175      32      70      50     689

  Fragments arranged by size: 

                 689     175     166      70      50      32
 
NspI r_CATG'y

Cuts at:      0     580    1182 
   Size:        580     602
 
PfoI T'CCnGG_A

Cuts at:      0    1046    1182 
   Size:       1046     136
 
PmlI CAC'GTG

Cuts at:      0     787    1182 
   Size:        787     395
 
PpuMI rG'GwC_Cy

Cuts at:      0      42     395    1182 
   Size:         42     353     787

  Fragments arranged by size: 

                 787     353      42
 
PstI C_TGCA'G

Cuts at:      0    1134    1182 
   Size:       1134      48
 
SanDI GG'GwC_CC

Cuts at:      0     395    1182 
   Size:        395     787
 
SapI GCTCTTCn'nnn_

Cuts at:      0     271    1182 
   Size:        271     911
 
SexAI A'CCwGG_T

Cuts at:      0      44    1182 
   Size:         44    1138
 
SfcI C'TryA_G

Cuts at:      0    1130    1182 
   Size:       1130      52
 
SmlI C'TyrA_G

Cuts at:      0     298    1182 
   Size:        298     884
 
StuI AGG'CCT

Cuts at:      0    1120    1182 
   Size:       1120      62
 
StyI C'CwwG_G

Cuts at:      0     101     327     710    1182 
   Size:        101     226     383     472

  Fragments arranged by size: 

                 472     383     226     101
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      86     862    1182 
   Size:         86     776     320

  Fragments arranged by size: 

                 776     320      86
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     987    1019    1182 
   Size:        987      32     163

  Fragments arranged by size: 

                 987     163      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      46    1182 
   Size:         46    1136
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      63    1182 
   Size:         63    1119
 
XmnI GAAnn'nnTTC

Cuts at:      0     187    1182 
   Size:        187     995
 

 Enzymes that do cut:

     AcuI     AleI     ApoI     BaeI    BbvCI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BseRI    BseYI     BsgI     BsmI   BstAPI
    BstXI    BtgZI     ClaI     DraI     DrdI     EarI  Eco57MI EcoO109I
     MslI     NspI     PfoI     PmlI    PpuMI     PstI    SanDI     SapI
    SexAI     SfcI     SmlI     StuI     StyI    TaqII     TstI  Tth111I
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI   DraIII     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     XbaI     XhoI
     XmaI     ZraI