(Linear) (Six-base) MAPSORT of: KIBB8283.seq  Check: 3220  from: 1  to: 876



KIBB8283.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     393     876 
   Size:         57     336     483

  Fragments arranged by size: 

                 483     336      57
 
AflIII A'CryG_T

Cuts at:      0     603     876 
   Size:        603     273
 
ApoI r'AATT_y

Cuts at:      0      23     275     863     876 
   Size:         23     252     588      13

  Fragments arranged by size: 

                 588     252      23      13
 
BanII G_rGCy'C

Cuts at:      0     673     876 
   Size:        673     203
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      63     876 
   Size:         63     813
 
BbvCI CC'TCA_GC

Cuts at:      0      10     876 
   Size:         10     866
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     692     876 
   Size:        692     184
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     557     876 
   Size:        305     252     319

  Fragments arranged by size: 

                 319     305     252
 
Bpu10I CC'TnA_GC

Cuts at:      0      10     876 
   Size:         10     866
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     828     876 
   Size:        828      48
 
BsiHKAI G_wGCw'C

Cuts at:      0     673     876 
   Size:        673     203
 
BsmI GAATG_Cn'

Cuts at:      0     643     876 
   Size:        643     233
 
Bsp1286I G_dGCh'C

Cuts at:      0     673     876 
   Size:        673     203
 
BsrDI GCAATG_nn'

Cuts at:      0     648     876 
   Size:        648     228
 
BsrGI T'GTAC_A

Cuts at:      0     600     876 
   Size:        600     276
 
BssSI C'ACGA_G

Cuts at:      0     114     876 
   Size:        114     762
 
BstBI TT'CG_AA

Cuts at:      0     215     876 
   Size:        215     661
 
BtsI GCAGTG_nn'

Cuts at:      0     816     876 
   Size:        816      60
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     630     665     876 
   Size:        630      35     211

  Fragments arranged by size: 

                 630     211      35
 
DraI TTT'AAA

Cuts at:      0     468     876 
   Size:        468     408
 
EaeI y'GGCC_r

Cuts at:      0     495     876 
   Size:        495     381
 
EcoICRI GAG'CTC

Cuts at:      0     671     876 
   Size:        671     205
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     305     393     557     876 
   Size:         57     248      88     164     319

  Fragments arranged by size: 

                 319     248     164      88      57
 
HindIII A'AGCT_T

Cuts at:      0     208     320     740     824     876 
   Size:        208     112     420      84      52

  Fragments arranged by size: 

                 420     208     112      84      52
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     446     876 
   Size:        446     430
 
MscI TGG'CCA

Cuts at:      0     497     876 
   Size:        497     379
 
MslI CAynn'nnrTG

Cuts at:      0     620     876 
   Size:        620     256
 
NspI r_CATG'y

Cuts at:      0     607     691     876 
   Size:        607      84     185

  Fragments arranged by size: 

                 607     185      84
 
PciI A'CATG_T

Cuts at:      0     603     876 
   Size:        603     273
 
SacI G_AGCT'C

Cuts at:      0     673     876 
   Size:        673     203
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     208     876 
   Size:        208     668
 
TatI w'GTAC_w

Cuts at:      0     600     876 
   Size:        600     276
 
XmnI GAAnn'nnTTC

Cuts at:      0     211     322     742     826     876 
   Size:        211     111     420      84      50

  Fragments arranged by size: 

                 420     211     111      84      50
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI    BanII     BbsI    BbvCI     BplI     BpmI
   Bpu10I     BsgI  BsiHKAI     BsmI Bsp1286I    BsrDI    BsrGI    BssSI
    BstBI     BtsI    CspCI     DraI     EaeI  EcoICRI  Eco57MI  HindIII
     MmeI     MscI     MslI     NspI     PciI     SacI    TaqII     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI   BssHII   BstAPI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI   DraIII     DrdI
     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI