Restriction map for
pF1KB8281
(Linear) (Six-base) MAPSORT of: KIBB8281.seq Check: 3692 from: 1 to: 915
KIBB8281.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 915
Size: 679 236
AfeI AGC'GCT
Cuts at: 0 201 915
Size: 201 714
AleI CACnn'nnGTG
Cuts at: 0 484 709 915
Size: 484 225 206
Fragments arranged by size:
484 225 206
ApaLI G'TGCA_C
Cuts at: 0 487 915
Size: 487 428
AvaI C'yCGr_G
Cuts at: 0 828 867 915
Size: 828 39 48
Fragments arranged by size:
828 48 39
AvrII C'CTAG_G
Cuts at: 0 727 915
Size: 727 188
BanII G_rGCy'C
Cuts at: 0 75 466 915
Size: 75 391 449
Fragments arranged by size:
449 391 75
BbvCI CC'TCA_GC
Cuts at: 0 300 915
Size: 300 615
BfrBI ATG'CAT
Cuts at: 0 591 915
Size: 591 324
BglI GCCn_nnn'nGGC
Cuts at: 0 652 715 786 915
Size: 652 63 71 129
Fragments arranged by size:
652 129 71 63
Bme1580I G_kGCm'C
Cuts at: 0 491 915
Size: 491 424
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 485 915
Size: 485 430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 273 309 327 879 915
Size: 243 30 36 18 552 36
Fragments arranged by size:
552 243 36 36 30 18
Bpu10I CC'TnA_GC
Cuts at: 0 252 300 315 473 915
Size: 252 48 15 158 442
Fragments arranged by size:
442 252 158 48 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 915
Size: 261 654
BsaI GGTCTCn'nnnn_
Cuts at: 0 233 800 915
Size: 233 567 115
Fragments arranged by size:
567 233 115
BsaAI yAC'GTr
Cuts at: 0 621 915
Size: 621 294
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 57 87 915
Size: 57 30 828
Fragments arranged by size:
828 57 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 825 897 915
Size: 825 72 18
Fragments arranged by size:
825 72 18
BsiEI CG_ry'CG
Cuts at: 0 174 915
Size: 174 741
BsiHKAI G_wGCw'C
Cuts at: 0 491 915
Size: 491 424
BsiWI C'GTAC_G
Cuts at: 0 617 915
Size: 617 298
Bsp1286I G_dGCh'C
Cuts at: 0 75 466 491 915
Size: 75 391 25 424
Fragments arranged by size:
424 391 75 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 782 827 915
Size: 782 45 88
Fragments arranged by size:
782 88 45
BsrBI CCG'CTC
Cuts at: 0 101 314 413 824 893 915
Size: 101 213 99 411 69 22
Fragments arranged by size:
411 213 101 99 69 22
BtgI C'CryG_G
Cuts at: 0 578 802 915
Size: 578 224 113
Fragments arranged by size:
578 224 113
BtsI GCAGTG_nn'
Cuts at: 0 288 698 915
Size: 288 410 217
Fragments arranged by size:
410 288 217
EaeI y'GGCC_r
Cuts at: 0 171 687 915
Size: 171 516 228
Fragments arranged by size:
516 228 171
EagI C'GGCC_G
Cuts at: 0 171 915
Size: 171 744
EarI CTCTTCn'nnn_
Cuts at: 0 19 300 363 915
Size: 19 281 63 552
Fragments arranged by size:
552 281 63 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 474 915
Size: 474 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 273 309 327 679 879 915
Size: 243 30 36 18 352 200 36
Fragments arranged by size:
352 243 200 36 36 30 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 726 915
Size: 726 189
EcoO109I rG'GnC_Cy
Cuts at: 0 818 839 915
Size: 818 21 76
Fragments arranged by size:
818 76 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 45 915
Size: 45 870
HaeII r_GCGC'y
Cuts at: 0 203 408 658 915
Size: 203 205 250 257
Fragments arranged by size:
257 250 205 203
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 507 539 915
Size: 97 32 378 32 376
Fragments arranged by size:
378 376 97 32 32
MscI TGG'CCA
Cuts at: 0 689 915
Size: 689 226
MslI CAynn'nnrTG
Cuts at: 0 484 577 668 709 915
Size: 484 93 91 41 206
Fragments arranged by size:
484 206 93 91 41
NsiI A_TGCA'T
Cuts at: 0 593 915
Size: 593 322
NspI r_CATG'y
Cuts at: 0 576 915
Size: 576 339
PpuMI rG'GwC_Cy
Cuts at: 0 818 839 915
Size: 818 21 76
Fragments arranged by size:
818 76 21
SapI GCTCTTCn'nnn_
Cuts at: 0 19 300 363 915
Size: 19 281 63 552
Fragments arranged by size:
552 281 63 19
SexAI A'CCwGG_T
Cuts at: 0 223 915
Size: 223 692
SmaI CCC'GGG
Cuts at: 0 869 915
Size: 869 46
SmlI C'TyrA_G
Cuts at: 0 276 915
Size: 276 639
SnaBI TAC'GTA
Cuts at: 0 621 915
Size: 621 294
StyI C'CwwG_G
Cuts at: 0 116 146 727 915
Size: 116 30 581 188
Fragments arranged by size:
581 188 116 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 221 915
Size: 221 694
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 57 89 915
Size: 57 32 826
Fragments arranged by size:
826 57 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 711 782 915
Size: 711 71 133
Fragments arranged by size:
711 133 71
XmaI C'CCGG_G
Cuts at: 0 867 915
Size: 867 48
Enzymes that do cut:
AcuI AfeI AleI ApaLI AvaI AvrII BanII BbvCI
BfrBI BglI Bme1580I BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaXI BseRI BsiEI BsiHKAI BsiWI Bsp1286I BspMI
BsrBI BtgI BtsI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I MscI MslI NsiI
NspI PpuMI SapI SexAI SmaI SmlI SnaBI StyI
TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI BaeI BamHI BanI BbeI BbsI BcgI BciVI
BclI BglII BlpI BmgBI BmrI BmtI BsaBI BsaHI
BsaWI BseYI BsgI BsmI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XhoI XmnI ZraI