Restriction map for
pF1KB8279
(Linear) (Six-base) MAPSORT of: KIBB8279.seq Check: 2350 from: 1 to: 1218
KIBB8279.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AccI GT'mk_AC
Cuts at: 0 543 1218
Size: 543 675
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 454 457 810 894 1218
Size: 428 26 3 353 84 324
Fragments arranged by size:
428 353 324 84 26 3
AfeI AGC'GCT
Cuts at: 0 1048 1218
Size: 1048 170
AflII C'TTAA_G
Cuts at: 0 28 1180 1218
Size: 28 1152 38
Fragments arranged by size:
1152 38 28
AhdI GACnn_n'nnGTC
Cuts at: 0 152 1218
Size: 152 1066
AleI CACnn'nnGTG
Cuts at: 0 1121 1218
Size: 1121 97
AvaI C'yCGr_G
Cuts at: 0 158 1218
Size: 158 1060
BanII G_rGCy'C
Cuts at: 0 284 1218
Size: 284 934
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 1218
Size: 56 1162
BbvCI CC'TCA_GC
Cuts at: 0 1000 1218
Size: 1000 218
BciVI GTATCCnnnnn_n'
Cuts at: 0 1022 1218
Size: 1022 196
BglII A'GATC_T
Cuts at: 0 345 1218
Size: 345 873
BmrI ACTGGGnnnn_n'
Cuts at: 0 794 1218
Size: 794 424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 271 368 722 890 1058 1218
Size: 268 3 97 354 168 168 160
Fragments arranged by size:
354 268 168 168 160 97 3
Bpu10I CC'TnA_GC
Cuts at: 0 202 1000 1218
Size: 202 798 218
Fragments arranged by size:
798 218 202
BsaI GGTCTCn'nnnn_
Cuts at: 0 1206 1218
Size: 1206 12
BsaBI GATnn'nnATC
Cuts at: 0 498 1218
Size: 498 720
BsaHI Gr'CG_yC
Cuts at: 0 711 1218
Size: 711 507
BsaWI w'CCGG_w
Cuts at: 0 309 1218
Size: 309 909
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 337 988 1218
Size: 292 45 651 230
Fragments arranged by size:
651 292 230 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1161 1170 1218
Size: 1161 9 48
Fragments arranged by size:
1161 48 9
BsiHKAI G_wGCw'C
Cuts at: 0 284 305 416 770 1218
Size: 284 21 111 354 448
Fragments arranged by size:
448 354 284 111 21
BsmI GAATG_Cn'
Cuts at: 0 976 1092 1218
Size: 976 116 126
Fragments arranged by size:
976 126 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 702 1218
Size: 702 516
Bsp1286I G_dGCh'C
Cuts at: 0 284 305 416 770 1218
Size: 284 21 111 354 448
Fragments arranged by size:
448 354 284 111 21
BspEI T'CCGG_A
Cuts at: 0 309 1218
Size: 309 909
BsrDI GCAATG_nn'
Cuts at: 0 729 927 1218
Size: 729 198 291
Fragments arranged by size:
729 291 198
BsrFI r'CCGG_y
Cuts at: 0 173 1218
Size: 173 1045
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1148 1218
Size: 1148 70
BstXI CCAn_nnnn'nTGG
Cuts at: 0 397 1218
Size: 397 821
BstYI r'GATC_y
Cuts at: 0 345 1218
Size: 345 873
BstZ17I GTA'TAC
Cuts at: 0 544 1218
Size: 544 674
Bsu36I CC'TnA_GG
Cuts at: 0 275 1218
Size: 275 943
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 711 746 963 998 1218
Size: 711 35 217 35 220
Fragments arranged by size:
711 220 217 35 35
EaeI y'GGCC_r
Cuts at: 0 99 1218
Size: 99 1119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 873 1218
Size: 873 345
EcoICRI GAG'CTC
Cuts at: 0 282 1218
Size: 282 936
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 271 368 428 454 457 722 810
Size: 268 3 97 60 26 3 265 88
Cuts at: 810 890 894 1058 1218
Size: 80 4 164 160
Fragments arranged by size:
268 265 164 160 97 88 80 60
26 4 3 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 274 1218
Size: 274 944
HaeII r_GCGC'y
Cuts at: 0 1050 1218
Size: 1050 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 138 170 550 582 1218
Size: 138 32 380 32 636
Fragments arranged by size:
636 380 138 32 32
HindIII A'AGCT_T
Cuts at: 0 738 1218
Size: 738 480
MscI TGG'CCA
Cuts at: 0 101 1218
Size: 101 1117
MslI CAynn'nnrTG
Cuts at: 0 395 801 969 1121 1218
Size: 395 406 168 152 97
Fragments arranged by size:
406 395 168 152 97
NaeI GCC'GGC
Cuts at: 0 175 1218
Size: 175 1043
NdeI CA'TA_TG
Cuts at: 0 798 966 1218
Size: 798 168 252
Fragments arranged by size:
798 252 168
NgoMIV G'CCGG_C
Cuts at: 0 173 1218
Size: 173 1045
PflMI CCAn_nnn'nTGG
Cuts at: 0 561 1218
Size: 561 657
SacI G_AGCT'C
Cuts at: 0 284 1218
Size: 284 934
ScaI AGT'ACT
Cuts at: 0 758 1218
Size: 758 460
SfcI C'TryA_G
Cuts at: 0 335 1218
Size: 335 883
SmlI C'TyrA_G
Cuts at: 0 28 1180 1218
Size: 28 1152 38
Fragments arranged by size:
1152 38 28
StuI AGG'CCT
Cuts at: 0 289 823 1218
Size: 289 534 395
Fragments arranged by size:
534 395 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 282 1218
Size: 282 936
TatI w'GTAC_w
Cuts at: 0 644 756 1218
Size: 644 112 462
Fragments arranged by size:
644 462 112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 148 313 1218
Size: 148 165 905
Fragments arranged by size:
905 165 148
XmnI GAAnn'nnTTC
Cuts at: 0 739 823 907 991 1218
Size: 739 84 84 84 227
Fragments arranged by size:
739 227 84 84 84
Enzymes that do cut:
AccI AcuI AfeI AflII AhdI AleI AvaI BanII
BbsI BbvCI BciVI BglII BmrI BpmI Bpu10I BsaI
BsaBI BsaHI BsaWI BseRI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BsrDI BsrFI BstAPI BstXI BstYI BstZ17I
Bsu36I CspCI EaeI EciI EcoICRI Eco57MI EcoNI HaeII
Hin4I HindIII MscI MslI NaeI NdeI NgoMIV PflMI
SacI ScaI SfcI SmlI StuI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BcgI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaAI BsaXI
BseYI BsiEI BsiWI BspHI BspMI BsrBI BsrGI BssHII
BssSI BstBI BstEII BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EagI EarI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NarI NcoI NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI