(Linear) (Six-base) MAPSORT of: KIBB8274.seq  Check: 8258  from: 1  to: 306



KIBB8274.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     256     306 
   Size:         70     186      50

  Fragments arranged by size: 

                 186      70      50
 
ApaI G_GGCC'C

Cuts at:      0     230     306 
   Size:        230      76
 
BamHI G'GATC_C

Cuts at:      0     267     306 
   Size:        267      39
 
BanII G_rGCy'C

Cuts at:      0     230     306 
   Size:        230      76
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     289     306 
   Size:        289      17
 
BbvCI CC'TCA_GC

Cuts at:      0     134     306 
   Size:        134     172
 
Bme1580I G_kGCm'C

Cuts at:      0     230     306 
   Size:        230      76
 
Bpu10I CC'TnA_GC

Cuts at:      0     134     306 
   Size:        134     172
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88     130     133     136     151     184     268     306 
   Size:         88      42       3       3      15      33      84      38

  Fragments arranged by size: 

                  88      84      42      38      33      15       3       3
            
 
BsiEI CG_ry'CG

Cuts at:      0      29     306 
   Size:         29     277
 
Bsp1286I G_dGCh'C

Cuts at:      0     230     306 
   Size:        230      76
 
BstYI r'GATC_y

Cuts at:      0     267     306 
   Size:        267      39
 
EaeI y'GGCC_r

Cuts at:      0      26     306 
   Size:         26     280
 
EagI C'GGCC_G

Cuts at:      0      26     306 
   Size:         26     280
 
EarI CTCTTCn'nnn_

Cuts at:      0     206     232     245     306 
   Size:        206      26      13      61

  Fragments arranged by size: 

                 206      61      26      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     256     306 
   Size:         70     186      50

  Fragments arranged by size: 

                 186      70      50
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45     226     306 
   Size:         45     181      80

  Fragments arranged by size: 

                 181      80      45
 
HaeII r_GCGC'y

Cuts at:      0      41     306 
   Size:         41     265
 
MspA1I CmG'CkG

Cuts at:      0      25     306 
   Size:         25     281
 
PpuMI rG'GwC_Cy

Cuts at:      0      45     306 
   Size:         45     261
 
PspOMI G'GGCC_C

Cuts at:      0     226     306 
   Size:        226      80
 
StyI C'CwwG_G

Cuts at:      0      41     306 
   Size:         41     265
 

 Enzymes that do cut:

     AcuI     ApaI    BamHI    BanII     BbsI    BbvCI Bme1580I   Bpu10I
    BseRI    BsiEI Bsp1286I    BstYI     EaeI     EagI     EarI  Eco57MI
 EcoO109I    HaeII   MspA1I    PpuMI   PspOMI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI