(Linear) (Six-base) MAPSORT of: KIBB8268.seq  Check: 8823  from: 1  to: 480



KIBB8268.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     480 
   Size:        250     230
 
BglII A'GATC_T

Cuts at:      0     204     480 
   Size:        204     276
 
BlpI GC'TnA_GC

Cuts at:      0     278     480 
   Size:        278     202
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     349     392     480 
   Size:        349      43      88

  Fragments arranged by size: 

                 349      88      43
 
BsiEI CG_ry'CG

Cuts at:      0       8     480 
   Size:          8     472
 
BsiHKAI G_wGCw'C

Cuts at:      0     128     480 
   Size:        128     352
 
BsmI GAATG_Cn'

Cuts at:      0     279     480 
   Size:        279     201
 
Bsp1286I G_dGCh'C

Cuts at:      0     128     480 
   Size:        128     352
 
BstYI r'GATC_y

Cuts at:      0     204     480 
   Size:        204     276
 
Bsu36I CC'TnA_GG

Cuts at:      0     245     480 
   Size:        245     235
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      24     480 
   Size:         24     456
 
EaeI y'GGCC_r

Cuts at:      0       5     480 
   Size:          5     475
 
EagI C'GGCC_G

Cuts at:      0       5     480 
   Size:          5     475
 
EarI CTCTTCn'nnn_

Cuts at:      0     116     173     329     480 
   Size:        116      57     156     151

  Fragments arranged by size: 

                 156     151     116      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     480 
   Size:        250     230
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     480 
   Size:        480
 
FspI TGC'GCA

Cuts at:      0      43     480 
   Size:         43     437
 
MfeI C'AATT_G

Cuts at:      0     371     480 
   Size:        371     109
 
SapI GCTCTTCn'nnn_

Cuts at:      0     116     173     480 
   Size:        116      57     307

  Fragments arranged by size: 

                 307     116      57
 
StyI C'CwwG_G

Cuts at:      0     359     480 
   Size:        359     121
 
XmnI GAAnn'nnTTC

Cuts at:      0     206     460     480 
   Size:        206     254      20

  Fragments arranged by size: 

                 254     206      20
 

 Enzymes that do cut:

     AcuI    BglII     BlpI     BsaI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BstYI   Bsu36I     DrdI     EaeI     EagI     EarI  Eco57MI     FalI
     FspI     MfeI     SapI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI