(Linear) (Six-base) MAPSORT of: KIBB8266.seq  Check: 4863  from: 1  to: 570



KIBB8266.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

ApaI G_GGCC'C

Cuts at:      0     415     570 
   Size:        415     155
 
ApoI r'AATT_y

Cuts at:      0     484     570 
   Size:        484      86
 
AvaI C'yCGr_G

Cuts at:      0     153     570 
   Size:        153     417
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     515     548     570 
   Size:        515      33      22

  Fragments arranged by size: 

                 515      33      22
 
BanII G_rGCy'C

Cuts at:      0     415     570 
   Size:        415     155
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     525     570 
   Size:        525      45
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     516     570 
   Size:        516      54
 
Bme1580I G_kGCm'C

Cuts at:      0     415     570 
   Size:        415     155
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     342     570 
   Size:        342     228
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      87     262     358     570 
   Size:         87     175      96     212

  Fragments arranged by size: 

                 212     175      96      87
 
BsaWI w'CCGG_w

Cuts at:      0      69     320     570 
   Size:         69     251     250

  Fragments arranged by size: 

                 251     250      69
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     223     253     570 
   Size:        223      30     317

  Fragments arranged by size: 

                 317     223      30
 
BseYI C'CCAG_C

Cuts at:      0      39      48     570 
   Size:         39       9     522

  Fragments arranged by size: 

                 522      39       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     570 
   Size:        109     461
 
Bsp1286I G_dGCh'C

Cuts at:      0     415     570 
   Size:        415     155
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      65     570 
   Size:         65     505
 
BsrGI T'GTAC_A

Cuts at:      0     392     570 
   Size:        392     178
 
Bsu36I CC'TnA_GG

Cuts at:      0     250     570 
   Size:        250     320
 
BtgI C'CryG_G

Cuts at:      0     215     293     570 
   Size:        215      78     277

  Fragments arranged by size: 

                 277     215      78
 
DraIII CAC_nnn'GTG

Cuts at:      0      20     570 
   Size:         20     550
 
EaeI y'GGCC_r

Cuts at:      0     434     570 
   Size:        434     136
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     249     570 
   Size:        249     321
 
EcoO109I rG'GnC_Cy

Cuts at:      0     299     411     412     570 
   Size:        299     112       1     158

  Fragments arranged by size: 

                 299     158     112       1
 
HaeII r_GCGC'y

Cuts at:      0     126     570 
   Size:        126     444
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     570 
   Size:        173     397
 
MscI TGG'CCA

Cuts at:      0     436     570 
   Size:        436     134
 
MspA1I CmG'CkG

Cuts at:      0     295     570 
   Size:        295     275
 
PasI CC'CwG_GG

Cuts at:      0     464     507     570 
   Size:        464      43      63

  Fragments arranged by size: 

                 464      63      43
 
PfoI T'CCnGG_A

Cuts at:      0     164     570 
   Size:        164     406
 
PpuMI rG'GwC_Cy

Cuts at:      0     299     570 
   Size:        299     271
 
PspOMI G'GGCC_C

Cuts at:      0     411     570 
   Size:        411     159
 
PspXI vC'TCGA_Gb

Cuts at:      0     153     570 
   Size:        153     417
 
PstI C_TGCA'G

Cuts at:      0      62     290     570 
   Size:         62     228     280

  Fragments arranged by size: 

                 280     228      62
 
SacII CC_GC'GG

Cuts at:      0     296     570 
   Size:        296     274
 
SanDI GG'GwC_CC

Cuts at:      0     299     570 
   Size:        299     271
 
SbfI CC_TGCA'GG

Cuts at:      0      62     570 
   Size:         62     508
 
SfcI C'TryA_G

Cuts at:      0      58     286     312     444     570 
   Size:         58     228      26     132     126

  Fragments arranged by size: 

                 228     132     126      58      26
 
SmlI C'TyrA_G

Cuts at:      0     153     570 
   Size:        153     417
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     382     570 
   Size:        382     188
 
TatI w'GTAC_w

Cuts at:      0     392     570 
   Size:        392     178
 
XhoI C'TCGA_G

Cuts at:      0     153     570 
   Size:        153     417
 

 Enzymes that do cut:

     ApaI     ApoI     AvaI     BaeI    BanII     BbsI    BciVI Bme1580I
     BmrI     BsaI    BsaWI    BsaXI    BseYI     BsgI Bsp1286I    BspMI
    BsrGI   Bsu36I     BtgI   DraIII     EaeI    EcoNI EcoO109I    HaeII
     MmeI     MscI   MspA1I     PasI     PfoI    PpuMI   PspOMI    PspXI
     PstI    SacII    SanDI     SbfI     SfcI     SmlI    TaqII     TatI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     AscI     AseI    AsiSI    AvrII    BamHI     BanI     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BseRI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI     ZraI