Restriction map for
pF1KB8261
(Linear) (Six-base) MAPSORT of: KIBB8261.seq Check: 9698 from: 1 to: 465
KIBB8261.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
Acc65I G'GTAC_C
Cuts at: 0 112 465
Size: 112 353
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 465
Size: 57 408
AvaI C'yCGr_G
Cuts at: 0 22 465
Size: 22 443
BanI G'GyrC_C
Cuts at: 0 52 112 280 358 465
Size: 52 60 168 78 107
Fragments arranged by size:
168 107 78 60 52
BbeI G_GCGC'C
Cuts at: 0 56 284 465
Size: 56 228 181
Fragments arranged by size:
228 181 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 63 465
Size: 63 402
BglI GCCn_nnn'nGGC
Cuts at: 0 305 465
Size: 305 160
Bme1580I G_kGCm'C
Cuts at: 0 361 465
Size: 361 104
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 465
Size: 166 299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 465
Size: 256 209
Bpu10I CC'TnA_GC
Cuts at: 0 289 301 465
Size: 289 12 164
Fragments arranged by size:
289 164 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 439 465
Size: 439 26
BsaBI GATnn'nnATC
Cuts at: 0 403 465
Size: 403 62
BsaHI Gr'CG_yC
Cuts at: 0 53 281 465
Size: 53 228 184
Fragments arranged by size:
228 184 53
BseYI C'CCAG_C
Cuts at: 0 276 365 465
Size: 276 89 100
Fragments arranged by size:
276 100 89
BsiEI CG_ry'CG
Cuts at: 0 62 145 465
Size: 62 83 320
Fragments arranged by size:
320 83 62
BsiHKAI G_wGCw'C
Cuts at: 0 422 465
Size: 422 43
Bsp1286I G_dGCh'C
Cuts at: 0 361 422 465
Size: 361 61 43
Fragments arranged by size:
361 61 43
BspHI T'CATG_A
Cuts at: 0 155 465
Size: 155 310
BsrBI CCG'CTC
Cuts at: 0 17 110 305 465
Size: 17 93 195 160
Fragments arranged by size:
195 160 93 17
BsrDI GCAATG_nn'
Cuts at: 0 119 465
Size: 119 346
BsrFI r'CCGG_y
Cuts at: 0 115 283 316 465
Size: 115 168 33 149
Fragments arranged by size:
168 149 115 33
BtgI C'CryG_G
Cuts at: 0 65 465
Size: 65 400
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 177 210 465
Size: 177 33 255
Fragments arranged by size:
255 177 33
EaeI y'GGCC_r
Cuts at: 0 59 125 142 465
Size: 59 66 17 323
Fragments arranged by size:
323 66 59 17
EagI C'GGCC_G
Cuts at: 0 59 142 465
Size: 59 83 323
Fragments arranged by size:
323 83 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 256 465
Size: 57 199 209
Fragments arranged by size:
209 199 57
HaeII r_GCGC'y
Cuts at: 0 56 80 89 284 465
Size: 56 24 9 195 181
Fragments arranged by size:
195 181 56 24 9
KasI G'GCGC_C
Cuts at: 0 52 280 465
Size: 52 228 185
Fragments arranged by size:
228 185 52
KpnI G_GTAC'C
Cuts at: 0 116 465
Size: 116 349
MslI CAynn'nnrTG
Cuts at: 0 172 465
Size: 172 293
MspA1I CmG'CkG
Cuts at: 0 276 465
Size: 276 189
NaeI GCC'GGC
Cuts at: 0 285 318 465
Size: 285 33 147
Fragments arranged by size:
285 147 33
NarI GG'CG_CC
Cuts at: 0 53 281 465
Size: 53 228 184
Fragments arranged by size:
228 184 53
NcoI C'CATG_G
Cuts at: 0 65 465
Size: 65 400
NgoMIV G'CCGG_C
Cuts at: 0 283 316 465
Size: 283 33 149
Fragments arranged by size:
283 149 33
PasI CC'CwG_GG
Cuts at: 0 81 465
Size: 81 384
PvuII CAG'CTG
Cuts at: 0 276 465
Size: 276 189
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 305 465
Size: 305 160
SfoI GGC'GCC
Cuts at: 0 54 282 465
Size: 54 228 183
Fragments arranged by size:
228 183 54
StyI C'CwwG_G
Cuts at: 0 65 465
Size: 65 400
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 204 333 465
Size: 171 33 129 132
Fragments arranged by size:
171 132 129 33
Enzymes that do cut:
Acc65I AcuI AvaI BanI BbeI BbsI BglI Bme1580I
BmrI BpmI Bpu10I BsaI BsaBI BsaHI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BsrBI BsrDI BsrFI BtgI BtgZI
EaeI EagI Eco57MI HaeII KasI KpnI MslI MspA1I
NaeI NarI NcoI NgoMIV PasI PvuII SfiI SfoI
StyI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanII BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaWI BsaXI BseRI BsgI BsiWI
BsmI BsmBI BspEI BspMI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI MfeI MluI MmeI MscI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI