Restriction map for
pF1KB8256
(Linear) (Six-base) MAPSORT of: KIBB8256.seq Check: 8952 from: 1 to: 1065
KIBB8256.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 509 1065
Size: 509 556
AccI GT'mk_AC
Cuts at: 0 47 832 1065
Size: 47 785 233
Fragments arranged by size:
785 233 47
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1065
Size: 622 443
AgeI A'CCGG_T
Cuts at: 0 995 1065
Size: 995 70
AhdI GACnn_n'nnGTC
Cuts at: 0 162 1065
Size: 162 903
AleI CACnn'nnGTG
Cuts at: 0 523 1065
Size: 523 542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 226 1065
Size: 226 839
AlwNI CAG_nnn'CTG
Cuts at: 0 75 765 1065
Size: 75 690 300
Fragments arranged by size:
690 300 75
ApoI r'AATT_y
Cuts at: 0 277 1065
Size: 277 788
AvaI C'yCGr_G
Cuts at: 0 635 1065
Size: 635 430
BanI G'GyrC_C
Cuts at: 0 497 639 1065
Size: 497 142 426
Fragments arranged by size:
497 426 142
BanII G_rGCy'C
Cuts at: 0 853 933 1065
Size: 853 80 132
Fragments arranged by size:
853 132 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 1026 1047 1065
Size: 1026 21 18
Fragments arranged by size:
1026 21 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 93 127 1065
Size: 93 34 938
Fragments arranged by size:
938 93 34
BglI GCCn_nnn'nGGC
Cuts at: 0 491 1065
Size: 491 574
BlpI GC'TnA_GC
Cuts at: 0 452 1065
Size: 452 613
Bme1580I G_kGCm'C
Cuts at: 0 642 1065
Size: 642 423
BmrI ACTGGGnnnn_n'
Cuts at: 0 124 1065
Size: 124 941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 648 867 1065
Size: 648 219 198
Fragments arranged by size:
648 219 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 442 1065
Size: 355 87 623
Fragments arranged by size:
623 355 87
Bpu10I CC'TnA_GC
Cuts at: 0 313 1065
Size: 313 752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 1065
Size: 952 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 61 534 1065
Size: 61 473 531
Fragments arranged by size:
531 473 61
BsaWI w'CCGG_w
Cuts at: 0 995 1065
Size: 995 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 178 208 1065
Size: 178 30 857
Fragments arranged by size:
857 178 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 627 1065
Size: 627 438
BseYI C'CCAG_C
Cuts at: 0 932 1065
Size: 932 133
Bsp1286I G_dGCh'C
Cuts at: 0 642 853 933 1065
Size: 642 211 80 132
Fragments arranged by size:
642 211 132 80
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 509 1065
Size: 509 556
BsrFI r'CCGG_y
Cuts at: 0 494 995 1065
Size: 494 501 70
Fragments arranged by size:
501 494 70
BstYI r'GATC_y
Cuts at: 0 962 1065
Size: 962 103
BtgI C'CryG_G
Cuts at: 0 7 1065
Size: 7 1058
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 96 1065
Size: 96 969
BtsI GCAGTG_nn'
Cuts at: 0 279 392 1065
Size: 279 113 673
Fragments arranged by size:
673 279 113
EaeI y'GGCC_r
Cuts at: 0 290 492 1065
Size: 290 202 573
Fragments arranged by size:
573 290 202
EarI CTCTTCn'nnn_
Cuts at: 0 392 1065
Size: 392 673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 442 622 1065
Size: 355 87 180 443
Fragments arranged by size:
443 355 180 87
EcoO109I rG'GnC_Cy
Cuts at: 0 72 1065
Size: 72 993
EcoRI G'AATT_C
Cuts at: 0 277 1065
Size: 277 788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 387 1065
Size: 387 678
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 178 180 210 556 588 732 764
Size: 148 30 2 30 346 32 144 32
Cuts at: 764 1065
Size: 301
Fragments arranged by size:
346 301 148 144 32 32 30 30
2
HincII GTy'rAC
Cuts at: 0 833 1065
Size: 833 232
MfeI C'AATT_G
Cuts at: 0 836 1065
Size: 836 229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 539 1016 1065
Size: 288 251 477 49
Fragments arranged by size:
477 288 251 49
MscI TGG'CCA
Cuts at: 0 292 1065
Size: 292 773
MslI CAynn'nnrTG
Cuts at: 0 286 523 736 1048 1065
Size: 286 237 213 312 17
Fragments arranged by size:
312 286 237 213 17
MspA1I CmG'CkG
Cuts at: 0 9 765 936 1065
Size: 9 756 171 129
Fragments arranged by size:
756 171 129 9
NaeI GCC'GGC
Cuts at: 0 496 1065
Size: 496 569
NgoMIV G'CCGG_C
Cuts at: 0 494 1065
Size: 494 571
PflMI CCAn_nnn'nTGG
Cuts at: 0 998 1065
Size: 998 67
PfoI T'CCnGG_A
Cuts at: 0 458 1065
Size: 458 607
PpuMI rG'GwC_Cy
Cuts at: 0 72 1065
Size: 72 993
PshAI GACnn'nnGTC
Cuts at: 0 70 139 1065
Size: 70 69 926
Fragments arranged by size:
926 70 69
PstI C_TGCA'G
Cuts at: 0 215 506 770 1065
Size: 215 291 264 295
Fragments arranged by size:
295 291 264 215
PvuII CAG'CTG
Cuts at: 0 765 936 1065
Size: 765 171 129
Fragments arranged by size:
765 171 129
SacII CC_GC'GG
Cuts at: 0 10 1065
Size: 10 1055
SalI G'TCGA_C
Cuts at: 0 831 1065
Size: 831 234
SapI GCTCTTCn'nnn_
Cuts at: 0 392 1065
Size: 392 673
ScaI AGT'ACT
Cuts at: 0 973 1065
Size: 973 92
SfcI C'TryA_G
Cuts at: 0 211 502 766 1065
Size: 211 291 264 299
Fragments arranged by size:
299 291 264 211
SmaI CCC'GGG
Cuts at: 0 637 1065
Size: 637 428
SmlI C'TyrA_G
Cuts at: 0 967 1065
Size: 967 98
StyI C'CwwG_G
Cuts at: 0 476 719 1065
Size: 476 243 346
Fragments arranged by size:
476 346 243
TatI w'GTAC_w
Cuts at: 0 971 1065
Size: 971 94
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 522 679 738 1065
Size: 522 157 59 327
Fragments arranged by size:
522 327 157 59
XmaI C'CCGG_G
Cuts at: 0 635 1065
Size: 635 430
XmnI GAAnn'nnTTC
Cuts at: 0 899 1065
Size: 899 166
Enzymes that do cut:
AarI AccI AcuI AgeI AhdI AleI AlfI AlwNI
ApoI AvaI BanI BanII BbsI BcgI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI
BsaXI BseRI BseYI Bsp1286I BspMI BsrFI BstYI BtgI
BtgZI BtsI EaeI EarI Eco57MI EcoO109I EcoRI FalI
Hin4I HincII MfeI MmeI MscI MslI MspA1I NaeI
NgoMIV PflMI PfoI PpuMI PshAI PstI PvuII SacII
SalI SapI ScaI SfcI SmaI SmlI StyI TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BciVI BclI BfrBI BglII BmgBI BmtI BsaAI
BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MluI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI
ZraI