(Linear) (Six-base) MAPSORT of: KIBB8250.seq  Check: 8336  from: 1  to: 366



KIBB8250.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     193     366 
   Size:         46     147     173

  Fragments arranged by size: 

                 173     147      46
 
AflIII A'CryG_T

Cuts at:      0     176     366 
   Size:        176     190
 
ApaI G_GGCC'C

Cuts at:      0     363     366 
   Size:        363       3
 
BanII G_rGCy'C

Cuts at:      0     363     366 
   Size:        363       3
 
BbvCI CC'TCA_GC

Cuts at:      0     323     366 
   Size:        323      43
 
Bme1580I G_kGCm'C

Cuts at:      0     363     366 
   Size:        363       3
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     366 
   Size:        322      44
 
Bpu10I CC'TnA_GC

Cuts at:      0     323     366 
   Size:        323      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     363     366 
   Size:        363       3
 
BspHI T'CATG_A

Cuts at:      0     152     366 
   Size:        152     214
 
BstYI r'GATC_y

Cuts at:      0      15     366 
   Size:         15     351
 
BtgI C'CryG_G

Cuts at:      0      11     366 
   Size:         11     355
 
EaeI y'GGCC_r

Cuts at:      0       2     366 
   Size:          2     364
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     193     322     366 
   Size:         46     147     129      44

  Fragments arranged by size: 

                 147     129      46      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0     359     366 
   Size:        359       7
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      51     366 
   Size:         51     315
 
MspA1I CmG'CkG

Cuts at:      0     237     366 
   Size:        237     129
 
NspI r_CATG'y

Cuts at:      0     130     180     366 
   Size:        130      50     186

  Fragments arranged by size: 

                 186     130      50
 
PciI A'CATG_T

Cuts at:      0     176     366 
   Size:        176     190
 
PfoI T'CCnGG_A

Cuts at:      0     335     366 
   Size:        335      31
 
PspOMI G'GGCC_C

Cuts at:      0     359     366 
   Size:        359       7
 
PvuII CAG'CTG

Cuts at:      0     237     366 
   Size:        237     129
 
SphI G_CATG'C

Cuts at:      0     130     366 
   Size:        130     236
 
StuI AGG'CCT

Cuts at:      0      34     366 
   Size:         34     332
 
StyI C'CwwG_G

Cuts at:      0     215     366 
   Size:        215     151
 
XbaI T'CTAG_A

Cuts at:      0      18     366 
   Size:         18     348
 
XmnI GAAnn'nnTTC

Cuts at:      0      34     224     366 
   Size:         34     190     142

  Fragments arranged by size: 

                 190     142      34
 

 Enzymes that do cut:

     AcuI   AflIII     ApaI    BanII    BbvCI Bme1580I     BpmI   Bpu10I
 Bsp1286I    BspHI    BstYI     BtgI     EaeI  Eco57MI EcoO109I     FalI
   MspA1I     NspI     PciI     PfoI   PspOMI    PvuII     SphI     StuI
     StyI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     ZraI