Restriction map for
pF1KB8248
(Linear) (Six-base) MAPSORT of: KIBB8248.seq Check: 2372 from: 1 to: 705
KIBB8248.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 421 705
Size: 421 284
AflIII A'CryG_T
Cuts at: 0 299 654 705
Size: 299 355 51
Fragments arranged by size:
355 299 51
AleI CACnn'nnGTG
Cuts at: 0 352 705
Size: 352 353
AlwNI CAG_nnn'CTG
Cuts at: 0 108 610 627 705
Size: 108 502 17 78
Fragments arranged by size:
502 108 78 17
AvaI C'yCGr_G
Cuts at: 0 62 530 705
Size: 62 468 175
Fragments arranged by size:
468 175 62
BamHI G'GATC_C
Cuts at: 0 501 705
Size: 501 204
BanII G_rGCy'C
Cuts at: 0 450 599 617 705
Size: 450 149 18 88
Fragments arranged by size:
450 149 88 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 651 705
Size: 651 54
BclI T'GATC_A
Cuts at: 0 290 705
Size: 290 415
BglI GCCn_nnn'nGGC
Cuts at: 0 415 705
Size: 415 290
Bme1580I G_kGCm'C
Cuts at: 0 325 440 705
Size: 325 115 265
Fragments arranged by size:
325 265 115
BmgBI CAC'GTC
Cuts at: 0 657 705
Size: 657 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 705
Size: 601 104
BsaAI yAC'GTr
Cuts at: 0 302 705
Size: 302 403
BsaHI Gr'CG_yC
Cuts at: 0 23 705
Size: 23 682
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 22 705
Size: 19 3 683
Fragments arranged by size:
683 19 3
BseYI C'CCAG_C
Cuts at: 0 318 705
Size: 318 387
BsiEI CG_ry'CG
Cuts at: 0 185 705
Size: 185 520
BsiHKAI G_wGCw'C
Cuts at: 0 617 705
Size: 617 88
BsmI GAATG_Cn'
Cuts at: 0 408 705
Size: 408 297
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4 705
Size: 4 701
Bsp1286I G_dGCh'C
Cuts at: 0 325 440 450 599 617 705
Size: 325 115 10 149 18 88
Fragments arranged by size:
325 149 115 88 18 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 351 421 705
Size: 116 235 70 284
Fragments arranged by size:
284 235 116 70
BsrBI CCG'CTC
Cuts at: 0 285 705
Size: 285 420
BsrFI r'CCGG_y
Cuts at: 0 180 376 705
Size: 180 196 329
Fragments arranged by size:
329 196 180
BssSI C'ACGA_G
Cuts at: 0 493 705
Size: 493 212
BstXI CCAn_nnnn'nTGG
Cuts at: 0 351 705
Size: 351 354
BstYI r'GATC_y
Cuts at: 0 501 705
Size: 501 204
Bsu36I CC'TnA_GG
Cuts at: 0 238 705
Size: 238 467
BtgI C'CryG_G
Cuts at: 0 350 705
Size: 350 355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 386 705
Size: 386 319
BtsI GCAGTG_nn'
Cuts at: 0 628 705
Size: 628 77
DrdI GACnn_nn'nnGTC
Cuts at: 0 157 705
Size: 157 548
EaeI y'GGCC_r
Cuts at: 0 118 182 705
Size: 118 64 523
Fragments arranged by size:
523 118 64
EagI C'GGCC_G
Cuts at: 0 182 705
Size: 182 523
EarI CTCTTCn'nnn_
Cuts at: 0 268 457 705
Size: 268 189 248
Fragments arranged by size:
268 248 189
EcoICRI GAG'CTC
Cuts at: 0 615 705
Size: 615 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 705
Size: 601 104
EcoO109I rG'GnC_Cy
Cuts at: 0 67 488 705
Size: 67 421 217
Fragments arranged by size:
421 217 67
FseI GG_CCGG'CC
Cuts at: 0 184 705
Size: 184 521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 493 525 705
Size: 364 32 97 32 180
Fragments arranged by size:
364 180 97 32 32
MslI CAynn'nnrTG
Cuts at: 0 349 352 705
Size: 349 3 353
Fragments arranged by size:
353 349 3
NaeI GCC'GGC
Cuts at: 0 182 378 705
Size: 182 196 327
Fragments arranged by size:
327 196 182
NgoMIV G'CCGG_C
Cuts at: 0 180 376 705
Size: 180 196 329
Fragments arranged by size:
329 196 180
PmlI CAC'GTG
Cuts at: 0 302 705
Size: 302 403
PpuMI rG'GwC_Cy
Cuts at: 0 67 488 705
Size: 67 421 217
Fragments arranged by size:
421 217 67
PstI C_TGCA'G
Cuts at: 0 79 362 686 705
Size: 79 283 324 19
Fragments arranged by size:
324 283 79 19
SacI G_AGCT'C
Cuts at: 0 617 705
Size: 617 88
SanDI GG'GwC_CC
Cuts at: 0 488 705
Size: 488 217
SapI GCTCTTCn'nnn_
Cuts at: 0 268 705
Size: 268 437
SfcI C'TryA_G
Cuts at: 0 75 358 682 705
Size: 75 283 324 23
Fragments arranged by size:
324 283 75 23
SmaI CCC'GGG
Cuts at: 0 532 705
Size: 532 173
SrfI GCCC'GGGC
Cuts at: 0 532 705
Size: 532 173
StyI C'CwwG_G
Cuts at: 0 590 705
Size: 590 115
XmaI C'CCGG_G
Cuts at: 0 530 705
Size: 530 175
Enzymes that do cut:
AarI AflIII AleI AlwNI AvaI BamHI BanII BbsI
BclI BglI Bme1580I BmgBI BpmI BsaAI BsaHI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FseI
Hin4I MslI NaeI NgoMIV PmlI PpuMI PstI SacI
SanDI SapI SfcI SmaI SrfI StyI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BsgI BsiWI BspEI BspHI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I ClaI
CspCI DraI DraIII EciI EcoNI EcoRI EcoRV FalI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MspA1I NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI