(Linear) (Six-base) MAPSORT of: KIBB8240.seq  Check: 8023  from: 1  to: 732



KIBB8240.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406     732 
   Size:        406     326
 
AflII C'TTAA_G

Cuts at:      0     457     732 
   Size:        457     275
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     569     601     732 
   Size:        569      32     131

  Fragments arranged by size: 

                 569     131      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     164     732 
   Size:        164     568
 
ApoI r'AATT_y

Cuts at:      0      70     138     605     732 
   Size:         70      68     467     127

  Fragments arranged by size: 

                 467     127      70      68
 
BanII G_rGCy'C

Cuts at:      0     635     732 
   Size:        635      97
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     104     134     732 
   Size:        104      30     598

  Fragments arranged by size: 

                 598     104      30
 
BclI T'GATC_A

Cuts at:      0     359     732 
   Size:        359     373
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     724     732 
   Size:        724       8
 
Bpu10I CC'TnA_GC

Cuts at:      0     509     732 
   Size:        509     223
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     569     599     732 
   Size:        569      30     133

  Fragments arranged by size: 

                 569     133      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     643     732 
   Size:        643      89
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     732 
   Size:        604     128
 
BsiHKAI G_wGCw'C

Cuts at:      0     635     732 
   Size:        635      97
 
Bsp1286I G_dGCh'C

Cuts at:      0     635     732 
   Size:        635      97
 
BspHI T'CATG_A

Cuts at:      0     435     732 
   Size:        435     297
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     425     732 
   Size:        425     307
 
BsrFI r'CCGG_y

Cuts at:      0      43     732 
   Size:         43     689
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     688     732 
   Size:        688      44
 
EcoICRI GAG'CTC

Cuts at:      0     633     732 
   Size:        633      99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406     732 
   Size:        406     326
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     493     732 
   Size:        493     239
 
HaeII r_GCGC'y

Cuts at:      0     489     732 
   Size:        489     243
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     207     239     417     449     732 
   Size:        207      32     178      32     283

  Fragments arranged by size: 

                 283     207     178      32      32
 
HincII GTy'rAC

Cuts at:      0      17     201     732 
   Size:         17     184     531

  Fragments arranged by size: 

                 531     184      17
 
HpaI GTT'AAC

Cuts at:      0     201     732 
   Size:        201     531
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     705     732 
   Size:         30     675      27

  Fragments arranged by size: 

                 675      30      27
 
NaeI GCC'GGC

Cuts at:      0      45     732 
   Size:         45     687
 
NgoMIV G'CCGG_C

Cuts at:      0      43     732 
   Size:         43     689
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     714     732 
   Size:        714      18
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     569     601     732 
   Size:        569      32     131

  Fragments arranged by size: 

                 569     131      32
 
SacI G_AGCT'C

Cuts at:      0     635     732 
   Size:        635      97
 
SexAI A'CCwGG_T

Cuts at:      0     446     732 
   Size:        446     286
 
SfcI C'TryA_G

Cuts at:      0     643     732 
   Size:        643      89
 
SmlI C'TyrA_G

Cuts at:      0     457     732 
   Size:        457     275
 

 Enzymes that do cut:

     AcuI    AflII     AloI    AlwNI     ApoI    BanII     BbsI     BclI
     BmrI   Bpu10I    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrFI   BstAPI  EcoICRI  Eco57MI    EcoNI    HaeII    Hin4I
   HincII     HpaI     MmeI     NaeI   NgoMIV    PflMI     PpiI     SacI
    SexAI     SfcI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI