(Linear) (Six-base) MAPSORT of: KIBB8236.seq  Check: 1537  from: 1  to: 882



KIBB8236.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     882 
   Size:        578     304
 
AflII C'TTAA_G

Cuts at:      0     297     405     882 
   Size:        297     108     477

  Fragments arranged by size: 

                 477     297     108
 
ApoI r'AATT_y

Cuts at:      0     799     882 
   Size:        799      83
 
BanII G_rGCy'C

Cuts at:      0      31     882 
   Size:         31     851
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     740     882 
   Size:        740     142
 
BglII A'GATC_T

Cuts at:      0     855     882 
   Size:        855      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     581     882 
   Size:        455     126     301

  Fragments arranged by size: 

                 455     301     126
 
BsaWI w'CCGG_w

Cuts at:      0     827     882 
   Size:        827      55
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     400     403     882 
   Size:        400       3     479

  Fragments arranged by size: 

                 479     400       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     119     882 
   Size:        119     763
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     119     882 
   Size:         31      88     763

  Fragments arranged by size: 

                 763      88      31
 
BspEI T'CCGG_A

Cuts at:      0     827     882 
   Size:        827      55
 
BsrDI GCAATG_nn'

Cuts at:      0     197     882 
   Size:        197     685
 
BsrGI T'GTAC_A

Cuts at:      0     246     882 
   Size:        246     636
 
BstYI r'GATC_y

Cuts at:      0     855     882 
   Size:        855      27
 
Bsu36I CC'TnA_GG

Cuts at:      0      34     882 
   Size:         34     848
 
BtsI GCAGTG_nn'

Cuts at:      0     871     882 
   Size:        871      11
 
EarI CTCTTCn'nnn_

Cuts at:      0     377     500     785     882 
   Size:        377     123     285      97

  Fragments arranged by size: 

                 377     285     123      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     578     581     882 
   Size:        455     123       3     301

  Fragments arranged by size: 

                 455     301     123       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0      38     718     882 
   Size:         38     680     164

  Fragments arranged by size: 

                 680     164      38
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     487     840     882 
   Size:        487     353      42

  Fragments arranged by size: 

                 487     353      42
 
HaeII r_GCGC'y

Cuts at:      0     571     882 
   Size:        571     311
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     355     387     882 
   Size:        355      32     495

  Fragments arranged by size: 

                 495     355      32
 
MslI CAynn'nnrTG

Cuts at:      0      22     332     882 
   Size:         22     310     550

  Fragments arranged by size: 

                 550     310      22
 
PpuMI rG'GwC_Cy

Cuts at:      0     718     882 
   Size:        718     164
 
SfcI C'TryA_G

Cuts at:      0     728     882 
   Size:        728     154
 
SmlI C'TyrA_G

Cuts at:      0     297     405     882 
   Size:        297     108     477

  Fragments arranged by size: 

                 477     297     108
 
StyI C'CwwG_G

Cuts at:      0     264     882 
   Size:        264     618
 
TatI w'GTAC_w

Cuts at:      0     246     882 
   Size:        246     636
 
XmnI GAAnn'nnTTC

Cuts at:      0     823     882 
   Size:        823      59
 

 Enzymes that do cut:

     AcuI    AflII     ApoI    BanII     BbsI    BglII     BpmI    BsaWI
    BseRI  BsiHKAI Bsp1286I    BspEI    BsrDI    BsrGI    BstYI   Bsu36I
     BtsI     EarI  Eco57MI EcoO109I     FalI    HaeII    Hin4I     MslI
    PpuMI     SfcI     SmlI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI