Restriction map for
pF1KB8235
(Linear) (Six-base) MAPSORT of: KIBB8235.seq Check: 6465 from: 1 to: 660
KIBB8235.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 621 660
Size: 621 39
AatII G_ACGT'C
Cuts at: 0 109 660
Size: 109 551
Acc65I G'GTAC_C
Cuts at: 0 248 660
Size: 248 412
AflIII A'CryG_T
Cuts at: 0 62 660
Size: 62 598
AlwNI CAG_nnn'CTG
Cuts at: 0 390 660
Size: 390 270
ApaI G_GGCC'C
Cuts at: 0 613 660
Size: 613 47
BanI G'GyrC_C
Cuts at: 0 248 598 633 660
Size: 248 350 35 27
Fragments arranged by size:
350 248 35 27
BanII G_rGCy'C
Cuts at: 0 613 660
Size: 613 47
BbeI G_GCGC'C
Cuts at: 0 602 660
Size: 602 58
BglII A'GATC_T
Cuts at: 0 221 660
Size: 221 439
Bme1580I G_kGCm'C
Cuts at: 0 613 660
Size: 613 47
BmtI G_CTAG'C
Cuts at: 0 460 660
Size: 460 200
BsaBI GATnn'nnATC
Cuts at: 0 76 660
Size: 76 584
BsaHI Gr'CG_yC
Cuts at: 0 106 599 660
Size: 106 493 61
Fragments arranged by size:
493 106 61
BsaWI w'CCGG_w
Cuts at: 0 251 660
Size: 251 409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 660
Size: 358 302
Bsp1286I G_dGCh'C
Cuts at: 0 613 660
Size: 613 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 621 660
Size: 621 39
BsrBI CCG'CTC
Cuts at: 0 246 426 660
Size: 246 180 234
Fragments arranged by size:
246 234 180
BsrFI r'CCGG_y
Cuts at: 0 449 660
Size: 449 211
BstYI r'GATC_y
Cuts at: 0 221 660
Size: 221 439
BtgI C'CryG_G
Cuts at: 0 161 660
Size: 161 499
BtsI GCAGTG_nn'
Cuts at: 0 341 660
Size: 341 319
DrdI GACnn_nn'nnGTC
Cuts at: 0 178 589 660
Size: 178 411 71
Fragments arranged by size:
411 178 71
EaeI y'GGCC_r
Cuts at: 0 447 660
Size: 447 213
EarI CTCTTCn'nnn_
Cuts at: 0 121 660
Size: 121 539
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 595 660
Size: 595 65
EcoO109I rG'GnC_Cy
Cuts at: 0 609 660
Size: 609 51
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 126 540 660
Size: 126 414 120
Fragments arranged by size:
414 126 120
HaeII r_GCGC'y
Cuts at: 0 284 497 534 602 660
Size: 284 213 37 68 58
Fragments arranged by size:
284 213 68 58 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 177 209 544 576 660
Size: 177 32 335 32 84
Fragments arranged by size:
335 177 84 32 32
KasI G'GCGC_C
Cuts at: 0 598 660
Size: 598 62
KpnI G_GTAC'C
Cuts at: 0 252 660
Size: 252 408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 660
Size: 551 109
MslI CAynn'nnrTG
Cuts at: 0 298 660
Size: 298 362
NaeI GCC'GGC
Cuts at: 0 451 660
Size: 451 209
NarI GG'CG_CC
Cuts at: 0 599 660
Size: 599 61
NgoMIV G'CCGG_C
Cuts at: 0 449 660
Size: 449 211
NheI G'CTAG_C
Cuts at: 0 456 660
Size: 456 204
NspI r_CATG'y
Cuts at: 0 66 660
Size: 66 594
PciI A'CATG_T
Cuts at: 0 62 660
Size: 62 598
PspOMI G'GGCC_C
Cuts at: 0 609 660
Size: 609 51
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 43 75 660
Size: 43 32 585
Fragments arranged by size:
585 43 32
PstI C_TGCA'G
Cuts at: 0 221 660
Size: 221 439
SfcI C'TryA_G
Cuts at: 0 217 660
Size: 217 443
SfoI GGC'GCC
Cuts at: 0 600 660
Size: 600 60
StyI C'CwwG_G
Cuts at: 0 468 497 660
Size: 468 29 163
Fragments arranged by size:
468 163 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 367 660
Size: 367 293
ZraI GAC'GTC
Cuts at: 0 107 660
Size: 107 553
Enzymes that do cut:
AarI AatII Acc65I AflIII AlwNI ApaI BanI BanII
BbeI BglII Bme1580I BmtI BsaBI BsaHI BsaWI BsgI
Bsp1286I BspMI BsrBI BsrFI BstYI BtgI BtsI DrdI
EaeI EarI EciI EcoO109I FalI HaeII Hin4I KasI
KpnI MmeI MslI NaeI NarI NgoMIV NheI NspI
PciI PspOMI PsrI PstI SfcI SfoI StyI TaqII
ZraI
Enzymes that do not cut:
AccI AclI AcuI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaXI BseRI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EagI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
MfeI MluI MscI MspA1I NcoI NdeI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI