Restriction map for
pF1KB8234
(Linear) (Six-base) MAPSORT of: KIBB8234.seq Check: 2546 from: 1 to: 774
KIBB8234.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
Acc65I G'GTAC_C
Cuts at: 0 596 774
Size: 596 178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 774
Size: 513 261
AflIII A'CryG_T
Cuts at: 0 277 774
Size: 277 497
AleI CACnn'nnGTG
Cuts at: 0 245 774
Size: 245 529
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 378 411 516 549 774
Size: 378 33 105 33 225
Fragments arranged by size:
378 225 105 33 33
BanI G'GyrC_C
Cuts at: 0 596 774
Size: 596 178
BanII G_rGCy'C
Cuts at: 0 164 774
Size: 164 610
BbsI GAAGACnn'nnnn_
Cuts at: 0 280 774
Size: 280 494
BbvCI CC'TCA_GC
Cuts at: 0 28 774
Size: 28 746
BmrI ACTGGGnnnn_n'
Cuts at: 0 755 774
Size: 755 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 575 774
Size: 575 199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 774
Size: 70 704
Bpu10I CC'TnA_GC
Cuts at: 0 28 774
Size: 28 746
BsaI GGTCTCn'nnnn_
Cuts at: 0 487 585 774
Size: 487 98 189
Fragments arranged by size:
487 189 98
BsaHI Gr'CG_yC
Cuts at: 0 7 774
Size: 7 767
BsaWI w'CCGG_w
Cuts at: 0 624 774
Size: 624 150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 90 120 413 443 774
Size: 90 30 293 30 331
Fragments arranged by size:
331 293 90 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 729 774
Size: 475 254 45
Fragments arranged by size:
475 254 45
BsiHKAI G_wGCw'C
Cuts at: 0 771 774
Size: 771 3
Bsp1286I G_dGCh'C
Cuts at: 0 164 771 774
Size: 164 607 3
Fragments arranged by size:
607 164 3
BsrFI r'CCGG_y
Cuts at: 0 650 774
Size: 650 124
BstEII G'GTnAC_C
Cuts at: 0 586 774
Size: 586 188
BstXI CCAn_nnnn'nTGG
Cuts at: 0 247 774
Size: 247 527
BtgI C'CryG_G
Cuts at: 0 514 601 774
Size: 514 87 173
Fragments arranged by size:
514 173 87
EarI CTCTTCn'nnn_
Cuts at: 0 58 671 774
Size: 58 613 103
Fragments arranged by size:
613 103 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 551 774
Size: 551 223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 513 774
Size: 70 443 261
Fragments arranged by size:
443 261 70
EcoO109I rG'GnC_Cy
Cuts at: 0 519 757 774
Size: 519 238 17
Fragments arranged by size:
519 238 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 744 774
Size: 112 32 600 30
Fragments arranged by size:
600 112 32 30
HincII GTy'rAC
Cuts at: 0 345 449 774
Size: 345 104 325
Fragments arranged by size:
345 325 104
KpnI G_GTAC'C
Cuts at: 0 600 774
Size: 600 174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 774
Size: 268 506
MslI CAynn'nnrTG
Cuts at: 0 245 774
Size: 245 529
MspA1I CmG'CkG
Cuts at: 0 264 730 774
Size: 264 466 44
Fragments arranged by size:
466 264 44
NaeI GCC'GGC
Cuts at: 0 652 774
Size: 652 122
NcoI C'CATG_G
Cuts at: 0 601 774
Size: 601 173
NgoMIV G'CCGG_C
Cuts at: 0 650 774
Size: 650 124
NspI r_CATG'y
Cuts at: 0 281 343 774
Size: 281 62 431
Fragments arranged by size:
431 281 62
PciI A'CATG_T
Cuts at: 0 277 774
Size: 277 497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 774
Size: 62 32 680
Fragments arranged by size:
680 62 32
PpuMI rG'GwC_Cy
Cuts at: 0 519 774
Size: 519 255
PsiI TTA'TAA
Cuts at: 0 152 774
Size: 152 622
StyI C'CwwG_G
Cuts at: 0 601 774
Size: 601 173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 90 122 413 445 774
Size: 90 32 291 32 329
Fragments arranged by size:
329 291 90 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 199 608 679 774
Size: 199 409 71 95
Fragments arranged by size:
409 199 95 71
Enzymes that do cut:
Acc65I AcuI AflIII AleI BaeI BanI BanII BbsI
BbvCI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BsiHKAI Bsp1286I BsrFI BstEII BstXI BtgI
EarI EciI Eco57MI EcoO109I Hin4I HincII KpnI MmeI
MslI MspA1I NaeI NcoI NgoMIV NspI PciI PpiI
PpuMI PsiI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BamHI BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BpuEI
BsaAI BsaBI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI MfeI MluI MscI NarI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI Tth111I XbaI XhoI XmaI XmnI ZraI