Restriction map for
pF1KB8228
(Linear) (Six-base) MAPSORT of: KIBB8228.seq Check: 8508 from: 1 to: 1227
KIBB8228.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 930 984 1053 1227
Size: 930 54 69 174
Fragments arranged by size:
930 174 69 54
ApoI r'AATT_y
Cuts at: 0 88 1227
Size: 88 1139
AvaI C'yCGr_G
Cuts at: 0 64 395 461 903 1227
Size: 64 331 66 442 324
Fragments arranged by size:
442 331 324 66 64
BamHI G'GATC_C
Cuts at: 0 920 1227
Size: 920 307
BanI G'GyrC_C
Cuts at: 0 318 768 1227
Size: 318 450 459
Fragments arranged by size:
459 450 318
BanII G_rGCy'C
Cuts at: 0 242 1227
Size: 242 985
BbsI GAAGACnn'nnnn_
Cuts at: 0 584 1227
Size: 584 643
BbvCI CC'TCA_GC
Cuts at: 0 872 1227
Size: 872 355
BglI GCCn_nnn'nGGC
Cuts at: 0 652 1227
Size: 652 575
BmrI ACTGGGnnnn_n'
Cuts at: 0 994 1227
Size: 994 233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 844 967 1078 1227
Size: 835 9 123 111 149
Fragments arranged by size:
835 149 123 111 9
Bpu10I CC'TnA_GC
Cuts at: 0 872 1227
Size: 872 355
BsaI GGTCTCn'nnnn_
Cuts at: 0 1028 1133 1227
Size: 1028 105 94
Fragments arranged by size:
1028 105 94
BsaHI Gr'CG_yC
Cuts at: 0 784 1227
Size: 784 443
BsaWI w'CCGG_w
Cuts at: 0 83 242 1211 1227
Size: 83 159 969 16
Fragments arranged by size:
969 159 83 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 448 832 964 1227
Size: 448 384 132 263
Fragments arranged by size:
448 384 263 132
BseYI C'CCAG_C
Cuts at: 0 77 647 1074 1227
Size: 77 570 427 153
Fragments arranged by size:
570 427 153 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 1227
Size: 41 1186
BsiHKAI G_wGCw'C
Cuts at: 0 242 1227
Size: 242 985
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1054 1227
Size: 1054 173
Bsp1286I G_dGCh'C
Cuts at: 0 242 1227
Size: 242 985
BspEI T'CCGG_A
Cuts at: 0 83 242 1227
Size: 83 159 985
Fragments arranged by size:
985 159 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1169 1227
Size: 1169 58
BsrBI CCG'CTC
Cuts at: 0 673 1227
Size: 673 554
BsrFI r'CCGG_y
Cuts at: 0 662 1227
Size: 662 565
BsrGI T'GTAC_A
Cuts at: 0 1145 1227
Size: 1145 82
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1053 1227
Size: 1053 174
BstEII G'GTnAC_C
Cuts at: 0 423 1227
Size: 423 804
BstYI r'GATC_y
Cuts at: 0 821 920 1227
Size: 821 99 307
Fragments arranged by size:
821 307 99
Bsu36I CC'TnA_GG
Cuts at: 0 35 590 1227
Size: 35 555 637
Fragments arranged by size:
637 555 35
BtsI GCAGTG_nn'
Cuts at: 0 347 1227
Size: 347 880
EarI CTCTTCn'nnn_
Cuts at: 0 230 347 1210 1227
Size: 230 117 863 17
Fragments arranged by size:
863 230 117 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 9 681 1016 1227
Size: 9 672 335 211
Fragments arranged by size:
672 335 211 9
EcoICRI GAG'CTC
Cuts at: 0 240 1227
Size: 240 987
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 844 967 1078 1227
Size: 835 9 123 111 149
Fragments arranged by size:
835 149 123 111 9
EcoO109I rG'GnC_Cy
Cuts at: 0 31 927 1011 1227
Size: 31 896 84 216
Fragments arranged by size:
896 216 84 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 811 813 829 843 845 861
Size: 346 32 433 2 16 14 2 16
Cuts at: 861 1024 1056 1072 1104 1227
Size: 163 32 16 32 123
Fragments arranged by size:
433 346 163 123 32 32 32 16
16 16 14 2 2
HincII GTy'rAC
Cuts at: 0 627 782 1227
Size: 627 155 445
Fragments arranged by size:
627 445 155
MspA1I CmG'CkG
Cuts at: 0 651 803 834 864 912 1050 1227
Size: 651 152 31 30 48 138 177
Fragments arranged by size:
651 177 152 138 48 31 30
NaeI GCC'GGC
Cuts at: 0 664 1227
Size: 664 563
NgoMIV G'CCGG_C
Cuts at: 0 662 1227
Size: 662 565
PflMI CCAn_nnn'nTGG
Cuts at: 0 930 1227
Size: 930 297
PfoI T'CCnGG_A
Cuts at: 0 916 923 1227
Size: 916 7 304
Fragments arranged by size:
916 304 7
PpuMI rG'GwC_Cy
Cuts at: 0 31 927 1011 1227
Size: 31 896 84 216
Fragments arranged by size:
896 216 84 31
PstI C_TGCA'G
Cuts at: 0 1121 1166 1227
Size: 1121 45 61
Fragments arranged by size:
1121 61 45
PvuII CAG'CTG
Cuts at: 0 651 864 912 1050 1227
Size: 651 213 48 138 177
Fragments arranged by size:
651 213 177 138 48
SacI G_AGCT'C
Cuts at: 0 242 1227
Size: 242 985
SapI GCTCTTCn'nnn_
Cuts at: 0 230 1210 1227
Size: 230 980 17
Fragments arranged by size:
980 230 17
SexAI A'CCwGG_T
Cuts at: 0 929 1227
Size: 929 298
SfcI C'TryA_G
Cuts at: 0 1117 1162 1227
Size: 1117 45 65
Fragments arranged by size:
1117 65 45
SmaI CCC'GGG
Cuts at: 0 905 1227
Size: 905 322
SmlI C'TyrA_G
Cuts at: 0 461 1227
Size: 461 766
SspI AAT'ATT
Cuts at: 0 195 1227
Size: 195 1032
StyI C'CwwG_G
Cuts at: 0 557 727 1007 1227
Size: 557 170 280 220
Fragments arranged by size:
557 280 220 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 336 786 1010 1227
Size: 336 450 224 217
Fragments arranged by size:
450 336 224 217
TatI w'GTAC_w
Cuts at: 0 1145 1227
Size: 1145 82
XhoI C'TCGA_G
Cuts at: 0 461 1227
Size: 461 766
XmaI C'CCGG_G
Cuts at: 0 903 1227
Size: 903 324
Enzymes that do cut:
AlwNI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BglI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrGI BstAPI BstEII BstYI Bsu36I BtsI EarI
EciI EcoICRI Eco57MI EcoO109I Hin4I HincII MspA1I NaeI
NgoMIV PflMI PfoI PpuMI PstI PvuII SacI SapI
SexAI SfcI SmaI SmlI SspI StyI TaqII TatI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaXI BsiEI BsiWI BsmI BspHI
BsrDI BssHII BssSI BstBI BstXI BstZ17I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XbaI XcmI
XmnI ZraI