(Linear) (Six-base) MAPSORT of: KIBB8218.seq  Check: 203  from: 1  to: 555



KIBB8218.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     292     555 
   Size:        292     263
 
AccI GT'mk_AC

Cuts at:      0     305     555 
   Size:        305     250
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     554     555 
   Size:        554       1
 
AlwNI CAG_nnn'CTG

Cuts at:      0      48     555 
   Size:         48     507
 
ApoI r'AATT_y

Cuts at:      0     424     555 
   Size:        424     131
 
AvaI C'yCGr_G

Cuts at:      0     416     555 
   Size:        416     139
 
BanI G'GyrC_C

Cuts at:      0      34     555 
   Size:         34     521
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     285     555 
   Size:        285     270
 
Bme1580I G_kGCm'C

Cuts at:      0      37     417     555 
   Size:         37     380     138

  Fragments arranged by size: 

                 380     138      37
 
BmgBI CAC'GTC

Cuts at:      0     184     555 
   Size:        184     371
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     357     555 
   Size:        357     198
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     423     555 
   Size:        423     132
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     393     555 
   Size:         73     320     162

  Fragments arranged by size: 

                 320     162      73
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     555 
   Size:        337     218
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     252     555 
   Size:        252     303
 
BsaBI GATnn'nnATC

Cuts at:      0      98     555 
   Size:         98     457
 
BsaHI Gr'CG_yC

Cuts at:      0      85     555 
   Size:         85     470
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     417     555 
   Size:         37     380     138

  Fragments arranged by size: 

                 380     138      37
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     292     555 
   Size:        292     263
 
BsrGI T'GTAC_A

Cuts at:      0     314     555 
   Size:        314     241
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      48     555 
   Size:         48     507
 
DraIII CAC_nnn'GTG

Cuts at:      0     365     555 
   Size:        365     190
 
EaeI y'GGCC_r

Cuts at:      0     455     555 
   Size:        455     100
 
EarI CTCTTCn'nnn_

Cuts at:      0     268     503     555 
   Size:        268     235      52

  Fragments arranged by size: 

                 268     235      52
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     393     554     555 
   Size:         73     320     161       1

  Fragments arranged by size: 

                 320     161      73       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     502     534     555 
   Size:        502      32      21

  Fragments arranged by size: 

                 502      32      21
 
MscI TGG'CCA

Cuts at:      0     457     555 
   Size:        457      98
 
MspA1I CmG'CkG

Cuts at:      0      48     105     555 
   Size:         48      57     450

  Fragments arranged by size: 

                 450      57      48
 
PstI C_TGCA'G

Cuts at:      0     110     555 
   Size:        110     445
 
PvuII CAG'CTG

Cuts at:      0     105     555 
   Size:        105     450
 
SfcI C'TryA_G

Cuts at:      0     106     555 
   Size:        106     449
 
SmaI CCC'GGG

Cuts at:      0     418     555 
   Size:        418     137
 
SmlI C'TyrA_G

Cuts at:      0     352     555 
   Size:        352     203
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     163     376     555 
   Size:        163     213     179

  Fragments arranged by size: 

                 213     179     163
 
TatI w'GTAC_w

Cuts at:      0     314     555 
   Size:        314     241
 
XmaI C'CCGG_G

Cuts at:      0     416     555 
   Size:        416     139
 
XmnI GAAnn'nnTTC

Cuts at:      0      19     555 
   Size:         19     536
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AlwNI     ApoI     AvaI     BanI     BbsI
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaBI
    BsaHI Bsp1286I    BspMI    BsrGI   BstAPI   DraIII     EaeI     EarI
  Eco57MI    Hin4I     MscI   MspA1I     PstI    PvuII     SfcI     SmaI
     SmlI    TaqII     TatI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI     BmtI   Bpu10I    BsaAI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI