(Linear) (Six-base) MAPSORT of: KIBB8216.seq  Check: 4762  from: 1  to: 645



KIBB8216.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AccI GT'mk_AC

Cuts at:      0     332     645 
   Size:        332     313
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     616     645 
   Size:        332     284      29

  Fragments arranged by size: 

                 332     284      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0      73      85     169     418     645 
   Size:         73      12      84     249     227

  Fragments arranged by size: 

                 249     227      84      73      12
 
ApoI r'AATT_y

Cuts at:      0     424     645 
   Size:        424     221
 
BanII G_rGCy'C

Cuts at:      0     343     594     645 
   Size:        343     251      51

  Fragments arranged by size: 

                 343     251      51
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     244     645 
   Size:        244     401
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     551     585     645 
   Size:        551      34      60

  Fragments arranged by size: 

                 551      60      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     157     645 
   Size:        157     488
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     256     645 
   Size:        256     389
 
BsaWI w'CCGG_w

Cuts at:      0     287     645 
   Size:        287     358
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     459     489     645 
   Size:        459      30     156

  Fragments arranged by size: 

                 459     156      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     505     645 
   Size:        505     140
 
BseYI C'CCAG_C

Cuts at:      0     152     395     645 
   Size:        152     243     250

  Fragments arranged by size: 

                 250     243     152
 
Bsp1286I G_dGCh'C

Cuts at:      0     343     594     645 
   Size:        343     251      51

  Fragments arranged by size: 

                 343     251      51
 
BspEI T'CCGG_A

Cuts at:      0     287     645 
   Size:        287     358
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     249     645 
   Size:        249     396
 
BsrGI T'GTAC_A

Cuts at:      0     483     645 
   Size:        483     162
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      45     645 
   Size:         45     600
 
ClaI AT'CG_AT

Cuts at:      0     176     645 
   Size:        176     469
 
EarI CTCTTCn'nnn_

Cuts at:      0     494     500     645 
   Size:        494       6     145

  Fragments arranged by size: 

                 494     145       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     616     645 
   Size:        332     284      29

  Fragments arranged by size: 

                 332     284      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     269     301     645 
   Size:         55      32     182      32     344

  Fragments arranged by size: 

                 344     182      55      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     502     645 
   Size:        190     312     143

  Fragments arranged by size: 

                 312     190     143
 
MspA1I CmG'CkG

Cuts at:      0     156     169     415     645 
   Size:        156      13     246     230

  Fragments arranged by size: 

                 246     230     156      13
 
PvuII CAG'CTG

Cuts at:      0     156     169     415     645 
   Size:        156      13     246     230

  Fragments arranged by size: 

                 246     230     156      13
 
SfcI C'TryA_G

Cuts at:      0      32     645 
   Size:         32     613
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     145     645 
   Size:        145     500
 
TatI w'GTAC_w

Cuts at:      0     483     645 
   Size:        483     162
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     459     491     645 
   Size:        459      32     154

  Fragments arranged by size: 

                 459     154      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      69     645 
   Size:         69     576
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI    BanII     BbsI     BcgI     BglI
     BmrI    BsaWI    BsaXI    BseRI    BseYI Bsp1286I    BspEI    BspMI
    BsrGI   BstAPI     ClaI     EarI  Eco57MI    Hin4I     MmeI   MspA1I
    PvuII     SfcI    TaqII     TatI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI