(Linear) (Six-base) MAPSORT of: KIBB8212.seq  Check: 8090  from: 1  to: 402



KIBB8212.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     298     329     402 
   Size:        143     155      31      73

  Fragments arranged by size: 

                 155     143      73      31
 
AflIII A'CryG_T

Cuts at:      0       5     402 
   Size:          5     397
 
AlwNI CAG_nnn'CTG

Cuts at:      0     166     402 
   Size:        166     236
 
ApoI r'AATT_y

Cuts at:      0     262     402 
   Size:        262     140
 
AvaI C'yCGr_G

Cuts at:      0     131     402 
   Size:        131     271
 
BanII G_rGCy'C

Cuts at:      0     329     402 
   Size:        329      73
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     100     402 
   Size:        100     302
 
BmgBI CAC'GTC

Cuts at:      0       6     402 
   Size:          6     396
 
BsaHI Gr'CG_yC

Cuts at:      0     107     402 
   Size:        107     295
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     191     221     402 
   Size:        191      30     181

  Fragments arranged by size: 

                 191     181      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     215     402 
   Size:        215     187
 
Bsp1286I G_dGCh'C

Cuts at:      0     329     402 
   Size:        329      73
 
BspHI T'CATG_A

Cuts at:      0      11     402 
   Size:         11     391
 
BstEII G'GTnAC_C

Cuts at:      0      75     402 
   Size:         75     327
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     246     402 
   Size:        246     156
 
BtgI C'CryG_G

Cuts at:      0      26      53     402 
   Size:         26      27     349

  Fragments arranged by size: 

                 349      27      26
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     213     402 
   Size:        213     189
 
EaeI y'GGCC_r

Cuts at:      0      29     402 
   Size:         29     373
 
EarI CTCTTCn'nnn_

Cuts at:      0     247     402 
   Size:        247     155
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     103     402 
   Size:        103     299
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     298     329     402 
   Size:        143     155      31      73

  Fragments arranged by size: 

                 155     143      73      31
 
EcoO109I rG'GnC_Cy

Cuts at:      0      18     360     402 
   Size:         18     342      42

  Fragments arranged by size: 

                 342      42      18
 
EcoRI G'AATT_C

Cuts at:      0     262     402 
   Size:        262     140
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      99     402 
   Size:         99     303
 
MscI TGG'CCA

Cuts at:      0      31     402 
   Size:         31     371
 
MspA1I CmG'CkG

Cuts at:      0      55     402 
   Size:         55     347
 
NcoI C'CATG_G

Cuts at:      0      26     402 
   Size:         26     376
 
PpuMI rG'GwC_Cy

Cuts at:      0     360     402 
   Size:        360      42
 
SacII CC_GC'GG

Cuts at:      0      56     402 
   Size:         56     346
 
StuI AGG'CCT

Cuts at:      0     187     402 
   Size:        187     215
 
StyI C'CwwG_G

Cuts at:      0      26      32      98     176     402 
   Size:         26       6      66      78     226

  Fragments arranged by size: 

                 226      78      66      26       6
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     191     223     402 
   Size:        191      32     179

  Fragments arranged by size: 

                 191     179      32
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     AvaI    BanII     BbsI    BmgBI
    BsaHI    BsaXI    BseRI Bsp1286I    BspHI   BstEII    BstXI     BtgI
    BtgZI     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRI     MmeI
     MscI   MspA1I     NcoI    PpuMI    SacII     StuI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI