Restriction map for
pF1KB8210
(Linear) (Six-base) MAPSORT of: KIBB8210.seq Check: 1782 from: 1 to: 1128
KIBB8210.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
ApoI r'AATT_y
Cuts at: 0 703 847 967 1128
Size: 703 144 120 161
Fragments arranged by size:
703 161 144 120
AseI AT'TA_AT
Cuts at: 0 223 1128
Size: 223 905
BanII G_rGCy'C
Cuts at: 0 582 1128
Size: 582 546
BfrBI ATG'CAT
Cuts at: 0 111 1128
Size: 111 1017
BglI GCCn_nnn'nGGC
Cuts at: 0 260 1128
Size: 260 868
BglII A'GATC_T
Cuts at: 0 1115 1128
Size: 1115 13
BmtI G_CTAG'C
Cuts at: 0 286 1128
Size: 286 842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 563 1128
Size: 338 225 565
Fragments arranged by size:
565 338 225
BsaBI GATnn'nnATC
Cuts at: 0 778 1128
Size: 778 350
BseYI C'CCAG_C
Cuts at: 0 258 525 1128
Size: 258 267 603
Fragments arranged by size:
603 267 258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1128
Size: 148 980
BsmI GAATG_Cn'
Cuts at: 0 1059 1128
Size: 1059 69
Bsp1286I G_dGCh'C
Cuts at: 0 582 1128
Size: 582 546
BspHI T'CATG_A
Cuts at: 0 885 1128
Size: 885 243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 540 781 987 1128
Size: 170 370 241 206 141
Fragments arranged by size:
370 241 206 170 141
BsrDI GCAATG_nn'
Cuts at: 0 26 932 1128
Size: 26 906 196
Fragments arranged by size:
906 196 26
BsrFI r'CCGG_y
Cuts at: 0 687 1128
Size: 687 441
BstAPI GCAn_nnn'nTGC
Cuts at: 0 697 1128
Size: 697 431
BstXI CCAn_nnnn'nTGG
Cuts at: 0 651 1128
Size: 651 477
BstYI r'GATC_y
Cuts at: 0 97 767 1046 1115 1128
Size: 97 670 279 69 13
Fragments arranged by size:
670 279 97 69 13
BtgI C'CryG_G
Cuts at: 0 479 1128
Size: 479 649
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 112 1128
Size: 112 1016
BtsI GCAGTG_nn'
Cuts at: 0 695 1128
Size: 695 433
DraI TTT'AAA
Cuts at: 0 140 1128
Size: 140 988
EaeI y'GGCC_r
Cuts at: 0 641 1128
Size: 641 487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 563 1128
Size: 338 225 565
Fragments arranged by size:
565 338 225
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1093 1128
Size: 1093 35
EcoO109I rG'GnC_Cy
Cuts at: 0 529 1128
Size: 529 599
EcoRI G'AATT_C
Cuts at: 0 847 1128
Size: 847 281
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 406 438 867 899 1128
Size: 292 32 82 32 429 32 229
Fragments arranged by size:
429 292 229 82 32 32 32
MspA1I CmG'CkG
Cuts at: 0 258 1128
Size: 258 870
NaeI GCC'GGC
Cuts at: 0 689 1128
Size: 689 439
NcoI C'CATG_G
Cuts at: 0 479 1128
Size: 479 649
NgoMIV G'CCGG_C
Cuts at: 0 687 1128
Size: 687 441
NheI G'CTAG_C
Cuts at: 0 282 1128
Size: 282 846
NsiI A_TGCA'T
Cuts at: 0 113 1128
Size: 113 1015
PasI CC'CwG_GG
Cuts at: 0 88 1128
Size: 88 1040
PflMI CCAn_nnn'nTGG
Cuts at: 0 94 1084 1128
Size: 94 990 44
Fragments arranged by size:
990 94 44
PpuMI rG'GwC_Cy
Cuts at: 0 529 1128
Size: 529 599
PsiI TTA'TAA
Cuts at: 0 863 1128
Size: 863 265
PvuII CAG'CTG
Cuts at: 0 258 1128
Size: 258 870
StyI C'CwwG_G
Cuts at: 0 479 1128
Size: 479 649
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 892 1128
Size: 892 236
TatI w'GTAC_w
Cuts at: 0 1066 1128
Size: 1066 62
Enzymes that do cut:
ApoI AseI BanII BfrBI BglI BglII BmtI BpmI
BsaBI BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI DraI
EaeI Eco57MI EcoNI EcoO109I EcoRI Hin4I MspA1I NaeI
NcoI NgoMIV NheI NsiI PasI PflMI PpuMI PsiI
PvuII StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmBI BspEI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraIII DrdI EagI EarI EciI EcoICRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NarI NdeI NotI NruI NspI PacI PciI PfoI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI