Restriction map for
pF1KB8209
(Linear) (Six-base) MAPSORT of: KIBB8209.seq Check: 4779 from: 1 to: 816
KIBB8209.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AccI GT'mk_AC
Cuts at: 0 372 816
Size: 372 444
AfeI AGC'GCT
Cuts at: 0 508 816
Size: 508 308
AflIII A'CryG_T
Cuts at: 0 219 816
Size: 219 597
AgeI A'CCGG_T
Cuts at: 0 242 816
Size: 242 574
AlwNI CAG_nnn'CTG
Cuts at: 0 277 657 816
Size: 277 380 159
Fragments arranged by size:
380 277 159
ApaI G_GGCC'C
Cuts at: 0 666 816
Size: 666 150
BanI G'GyrC_C
Cuts at: 0 391 816
Size: 391 425
BanII G_rGCy'C
Cuts at: 0 666 702 746 768 816
Size: 666 36 44 22 48
Fragments arranged by size:
666 48 44 36 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 216 816
Size: 216 600
BbvCI CC'TCA_GC
Cuts at: 0 236 466 816
Size: 236 230 350
Fragments arranged by size:
350 236 230
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 30 816
Size: 30 786
BglI GCCn_nnn'nGGC
Cuts at: 0 617 816
Size: 617 199
Bme1580I G_kGCm'C
Cuts at: 0 396 666 816
Size: 396 270 150
Fragments arranged by size:
396 270 150
BmgBI CAC'GTC
Cuts at: 0 183 691 816
Size: 183 508 125
Fragments arranged by size:
508 183 125
Bpu10I CC'TnA_GC
Cuts at: 0 236 466 816
Size: 236 230 350
Fragments arranged by size:
350 236 230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 512 531 816
Size: 418 94 19 285
Fragments arranged by size:
418 285 94 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 535 816
Size: 535 281
BsaWI w'CCGG_w
Cuts at: 0 242 816
Size: 242 574
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 94 816
Size: 91 3 722
Fragments arranged by size:
722 91 3
BseYI C'CCAG_C
Cuts at: 0 635 816
Size: 635 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 816
Size: 163 653
BsiEI CG_ry'CG
Cuts at: 0 593 816
Size: 593 223
BsiHKAI G_wGCw'C
Cuts at: 0 476 816
Size: 476 340
BsmBI CGTCTCn'nnnn_
Cuts at: 0 246 816
Size: 246 570
Bsp1286I G_dGCh'C
Cuts at: 0 396 476 666 702 746 768 816
Size: 396 80 190 36 44 22 48
Fragments arranged by size:
396 190 80 48 44 36 22
BsrBI CCG'CTC
Cuts at: 0 517 731 816
Size: 517 214 85
Fragments arranged by size:
517 214 85
BsrFI r'CCGG_y
Cuts at: 0 242 816
Size: 242 574
BstAPI GCAn_nnn'nTGC
Cuts at: 0 277 816
Size: 277 539
BstXI CCAn_nnnn'nTGG
Cuts at: 0 360 816
Size: 360 456
BstYI r'GATC_y
Cuts at: 0 755 816
Size: 755 61
EaeI y'GGCC_r
Cuts at: 0 365 590 679 749 816
Size: 365 225 89 70 67
Fragments arranged by size:
365 225 89 70 67
EagI C'GGCC_G
Cuts at: 0 590 816
Size: 590 226
EcoO109I rG'GnC_Cy
Cuts at: 0 600 662 793 816
Size: 600 62 131 23
Fragments arranged by size:
600 131 62 23
FspI TGC'GCA
Cuts at: 0 499 816
Size: 499 317
HaeII r_GCGC'y
Cuts at: 0 203 492 510 816
Size: 203 289 18 306
Fragments arranged by size:
306 289 203 18
MluI A'CGCG_T
Cuts at: 0 219 816
Size: 219 597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 758 816
Size: 195 563 58
Fragments arranged by size:
563 195 58
MscI TGG'CCA
Cuts at: 0 751 816
Size: 751 65
MslI CAynn'nnrTG
Cuts at: 0 385 816
Size: 385 431
MspA1I CmG'CkG
Cuts at: 0 45 277 816
Size: 45 232 539
Fragments arranged by size:
539 232 45
PfoI T'CCnGG_A
Cuts at: 0 622 816
Size: 622 194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 816
Size: 229 32 555
Fragments arranged by size:
555 229 32
PpuMI rG'GwC_Cy
Cuts at: 0 600 793 816
Size: 600 193 23
Fragments arranged by size:
600 193 23
PspOMI G'GGCC_C
Cuts at: 0 662 816
Size: 662 154
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 421 453 816
Size: 421 32 363
Fragments arranged by size:
421 363 32
PvuII CAG'CTG
Cuts at: 0 277 816
Size: 277 539
SanDI GG'GwC_CC
Cuts at: 0 600 816
Size: 600 216
ScaI AGT'ACT
Cuts at: 0 439 816
Size: 439 377
SexAI A'CCwGG_T
Cuts at: 0 454 816
Size: 454 362
SmlI C'TyrA_G
Cuts at: 0 433 510 527 816
Size: 433 77 17 289
Fragments arranged by size:
433 289 77 17
StyI C'CwwG_G
Cuts at: 0 305 816
Size: 305 511
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 315 379 559 816
Size: 315 64 180 257
Fragments arranged by size:
315 257 180 64
TatI w'GTAC_w
Cuts at: 0 437 816
Size: 437 379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 620 816
Size: 620 196
Enzymes that do cut:
AccI AfeI AflIII AgeI AlwNI ApaI BanI BanII
BbsI BbvCI BcgI BglI Bme1580I BmgBI Bpu10I BpuEI
BsaI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BsrBI BsrFI BstAPI BstXI BstYI EaeI EagI
EcoO109I FspI HaeII MluI MmeI MscI MslI MspA1I
PfoI PpiI PpuMI PspOMI PsrI PvuII SanDI ScaI
SexAI SmlI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BplI BpmI BsaAI BsaBI BsaHI
BsaXI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PshAI
PsiI PspXI PstI PvuI RsrII SacI SacII SalI
SapI SbfI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI