Restriction map for
pF1KB8207
(Linear) (Six-base) MAPSORT of: KIBB8207.seq Check: 2251 from: 1 to: 876
KIBB8207.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AatII G_ACGT'C
Cuts at: 0 206 876
Size: 206 670
Acc65I G'GTAC_C
Cuts at: 0 497 876
Size: 497 379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 280 400 820 876
Size: 193 87 120 420 56
Fragments arranged by size:
420 193 120 87 56
AflIII A'CryG_T
Cuts at: 0 551 876
Size: 551 325
AhdI GACnn_n'nnGTC
Cuts at: 0 555 876
Size: 555 321
AlwNI CAG_nnn'CTG
Cuts at: 0 258 393 876
Size: 258 135 483
Fragments arranged by size:
483 258 135
ApoI r'AATT_y
Cuts at: 0 241 876
Size: 241 635
AvaI C'yCGr_G
Cuts at: 0 443 876
Size: 443 433
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 481 514 876
Size: 481 33 362
Fragments arranged by size:
481 362 33
BanI G'GyrC_C
Cuts at: 0 37 497 876
Size: 37 460 379
Fragments arranged by size:
460 379 37
BbvCI CC'TCA_GC
Cuts at: 0 479 876
Size: 479 397
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 603 637 876
Size: 603 34 239
Fragments arranged by size:
603 239 34
BglII A'GATC_T
Cuts at: 0 152 876
Size: 152 724
Bme1580I G_kGCm'C
Cuts at: 0 758 876
Size: 758 118
BmgBI CAC'GTC
Cuts at: 0 542 876
Size: 542 334
Bpu10I CC'TnA_GC
Cuts at: 0 479 876
Size: 479 397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 876
Size: 148 728
BsaHI Gr'CG_yC
Cuts at: 0 203 661 876
Size: 203 458 215
Fragments arranged by size:
458 215 203
BsaWI w'CCGG_w
Cuts at: 0 507 876
Size: 507 369
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 876
Size: 685 191
BseYI C'CCAG_C
Cuts at: 0 339 592 654 876
Size: 339 253 62 222
Fragments arranged by size:
339 253 222 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 876
Size: 193 683
Bsp1286I G_dGCh'C
Cuts at: 0 758 876
Size: 758 118
BspHI T'CATG_A
Cuts at: 0 78 198 876
Size: 78 120 678
Fragments arranged by size:
678 120 78
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 401 876
Size: 401 475
BsrDI GCAATG_nn'
Cuts at: 0 288 366 621 876
Size: 288 78 255 255
Fragments arranged by size:
288 255 255 78
BsrFI r'CCGG_y
Cuts at: 0 562 876
Size: 562 314
BstEII G'GTnAC_C
Cuts at: 0 286 876
Size: 286 590
BstYI r'GATC_y
Cuts at: 0 152 641 780 876
Size: 152 489 139 96
Fragments arranged by size:
489 152 139 96
BtsI GCAGTG_nn'
Cuts at: 0 682 876
Size: 682 194
DraIII CAC_nnn'GTG
Cuts at: 0 494 876
Size: 494 382
EaeI y'GGCC_r
Cuts at: 0 584 728 876
Size: 584 144 148
Fragments arranged by size:
584 148 144
EarI CTCTTCn'nnn_
Cuts at: 0 325 631 827 876
Size: 325 306 196 49
Fragments arranged by size:
325 306 196 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 129 860 876
Size: 129 731 16
Fragments arranged by size:
731 129 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 280 400 820 876
Size: 193 87 120 420 56
Fragments arranged by size:
420 193 120 87 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 163 876
Size: 163 713
EcoO109I rG'GnC_Cy
Cuts at: 0 255 375 837 876
Size: 255 120 462 39
Fragments arranged by size:
462 255 120 39
EcoRI G'AATT_C
Cuts at: 0 241 876
Size: 241 635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 660 876
Size: 660 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 75 107 541 573 876
Size: 75 32 434 32 303
Fragments arranged by size:
434 303 75 32 32
KpnI G_GTAC'C
Cuts at: 0 501 876
Size: 501 375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 876
Size: 672 204
MscI TGG'CCA
Cuts at: 0 586 876
Size: 586 290
MslI CAynn'nnrTG
Cuts at: 0 550 876
Size: 550 326
MspA1I CmG'CkG
Cuts at: 0 467 478 876
Size: 467 11 398
Fragments arranged by size:
467 398 11
NaeI GCC'GGC
Cuts at: 0 564 876
Size: 564 312
NgoMIV G'CCGG_C
Cuts at: 0 562 876
Size: 562 314
NspI r_CATG'y
Cuts at: 0 555 876
Size: 555 321
PciI A'CATG_T
Cuts at: 0 551 876
Size: 551 325
PfoI T'CCnGG_A
Cuts at: 0 146 876
Size: 146 730
PpuMI rG'GwC_Cy
Cuts at: 0 255 375 876
Size: 255 120 501
Fragments arranged by size:
501 255 120
PshAI GACnn'nnGTC
Cuts at: 0 866 876
Size: 866 10
PspXI vC'TCGA_Gb
Cuts at: 0 443 876
Size: 443 433
PstI C_TGCA'G
Cuts at: 0 791 845 876
Size: 791 54 31
Fragments arranged by size:
791 54 31
PvuII CAG'CTG
Cuts at: 0 478 876
Size: 478 398
SfcI C'TryA_G
Cuts at: 0 787 841 876
Size: 787 54 35
Fragments arranged by size:
787 54 35
SmlI C'TyrA_G
Cuts at: 0 163 443 876
Size: 163 280 433
Fragments arranged by size:
433 280 163
TatI w'GTAC_w
Cuts at: 0 533 876
Size: 533 343
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 497 529 876
Size: 497 32 347
Fragments arranged by size:
497 347 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 738 876
Size: 738 138
XhoI C'TCGA_G
Cuts at: 0 443 876
Size: 443 433
ZraI GAC'GTC
Cuts at: 0 204 876
Size: 204 672
Enzymes that do cut:
AatII Acc65I AcuI AflIII AhdI AlwNI ApoI AvaI
BaeI BanI BbvCI BcgI BglII Bme1580I BmgBI Bpu10I
BpuEI BsaHI BsaWI BseRI BseYI BsgI Bsp1286I BspHI
BspMI BsrDI BsrFI BstEII BstYI BtsI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
KpnI MmeI MscI MslI MspA1I NaeI NgoMIV NspI
PciI PfoI PpuMI PshAI PspXI PstI PvuII SfcI
SmlI TatI TstI XcmI XhoI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BamHI
BanII BbeI BbsI BciVI BclI BfrBI BglI BlpI
BmrI BmtI BplI BpmI BsaI BsaAI BsaBI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PsiI
PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII Tth111I XbaI XmaI XmnI