Restriction map for
pF1KB8205
(Linear) (Six-base) MAPSORT of: KIBB8205.seq Check: 5085 from: 1 to: 1053
KIBB8205.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
Acc65I G'GTAC_C
Cuts at: 0 494 860 1053
Size: 494 366 193
Fragments arranged by size:
494 366 193
AleI CACnn'nnGTG
Cuts at: 0 442 1053
Size: 442 611
AlwNI CAG_nnn'CTG
Cuts at: 0 840 1000 1053
Size: 840 160 53
Fragments arranged by size:
840 160 53
ApaI G_GGCC'C
Cuts at: 0 304 750 1053
Size: 304 446 303
Fragments arranged by size:
446 304 303
AvaI C'yCGr_G
Cuts at: 0 355 1053
Size: 355 698
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 478 511 1053
Size: 478 33 542
Fragments arranged by size:
542 478 33
BamHI G'GATC_C
Cuts at: 0 564 593 1053
Size: 564 29 460
Fragments arranged by size:
564 460 29
BanI G'GyrC_C
Cuts at: 0 3 119 140 148 444 494 509 755
Size: 3 116 21 8 296 50 15 246
Cuts at: 755 860 1053
Size: 105 193
Fragments arranged by size:
296 246 193 116 105 50 21 15
8 3
BanII G_rGCy'C
Cuts at: 0 62 304 341 750 1053
Size: 62 242 37 409 303
Fragments arranged by size:
409 303 242 62 37
BbeI G_GCGC'C
Cuts at: 0 7 513 1053
Size: 7 506 540
Fragments arranged by size:
540 506 7
BglI GCCn_nnn'nGGC
Cuts at: 0 253 1053
Size: 253 800
Bme1580I G_kGCm'C
Cuts at: 0 122 226 304 455 750 1053
Size: 122 104 78 151 295 303
Fragments arranged by size:
303 295 151 122 104 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 811 929 1053
Size: 811 118 124
Fragments arranged by size:
811 124 118
BmtI G_CTAG'C
Cuts at: 0 364 1053
Size: 364 689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 793 898 1053
Size: 418 375 105 155
Fragments arranged by size:
418 375 155 105
Bpu10I CC'TnA_GC
Cuts at: 0 136 1053
Size: 136 917
BsaI GGTCTCn'nnnn_
Cuts at: 0 907 1053
Size: 907 146
BsaHI Gr'CG_yC
Cuts at: 0 4 98 510 1053
Size: 4 94 412 543
Fragments arranged by size:
543 412 94 4
BsaWI w'CCGG_w
Cuts at: 0 590 1053
Size: 590 463
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 97 415 923 1018 1053
Size: 58 39 318 508 95 35
Fragments arranged by size:
508 318 95 58 39 35
BsiHKAI G_wGCw'C
Cuts at: 0 779 848 1053
Size: 779 69 205
Fragments arranged by size:
779 205 69
BsmI GAATG_Cn'
Cuts at: 0 270 1053
Size: 270 783
Bsp1286I G_dGCh'C
Cuts at: 0 62 122 226 304 341 455 750 779
Size: 62 60 104 78 37 114 295 29
Cuts at: 779 848 1053
Size: 69 205
Fragments arranged by size:
295 205 114 104 78 69 62 60
37 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 872 1053
Size: 872 181
BsrDI GCAATG_nn'
Cuts at: 0 609 1053
Size: 609 444
BsrFI r'CCGG_y
Cuts at: 0 6 1053
Size: 6 1047
BstEII G'GTnAC_C
Cuts at: 0 571 1053
Size: 571 482
BstXI CCAn_nnnn'nTGG
Cuts at: 0 471 1053
Size: 471 582
BstYI r'GATC_y
Cuts at: 0 564 593 1038 1053
Size: 564 29 445 15
Fragments arranged by size:
564 445 29 15
Bsu36I CC'TnA_GG
Cuts at: 0 1001 1053
Size: 1001 52
BtgI C'CryG_G
Cuts at: 0 513 1053
Size: 513 540
BtsI GCAGTG_nn'
Cuts at: 0 498 1053
Size: 498 555
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 1053
Size: 34 1019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 793 898 1053
Size: 418 375 105 155
Fragments arranged by size:
418 375 155 105
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 832 1053
Size: 187 645 221
Fragments arranged by size:
645 221 187
EcoO109I rG'GnC_Cy
Cuts at: 0 294 405 633 1008 1053
Size: 294 111 228 375 45
Fragments arranged by size:
375 294 228 111 45
HaeII r_GCGC'y
Cuts at: 0 7 88 397 513 1053
Size: 7 81 309 116 540
Fragments arranged by size:
540 309 116 81 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1027 1053
Size: 1027 26
KasI G'GCGC_C
Cuts at: 0 3 509 1053
Size: 3 506 544
Fragments arranged by size:
544 506 3
KpnI G_GTAC'C
Cuts at: 0 498 864 1053
Size: 498 366 189
Fragments arranged by size:
498 366 189
MslI CAynn'nnrTG
Cuts at: 0 442 1053
Size: 442 611
MspA1I CmG'CkG
Cuts at: 0 205 306 1053
Size: 205 101 747
Fragments arranged by size:
747 205 101
NarI GG'CG_CC
Cuts at: 0 4 510 1053
Size: 4 506 543
Fragments arranged by size:
543 506 4
NheI G'CTAG_C
Cuts at: 0 360 1053
Size: 360 693
NspI r_CATG'y
Cuts at: 0 813 1053
Size: 813 240
PasI CC'CwG_GG
Cuts at: 0 918 1053
Size: 918 135
PpuMI rG'GwC_Cy
Cuts at: 0 294 633 1053
Size: 294 339 420
Fragments arranged by size:
420 339 294
PspOMI G'GGCC_C
Cuts at: 0 300 746 1053
Size: 300 446 307
Fragments arranged by size:
446 307 300
PstI C_TGCA'G
Cuts at: 0 35 836 991 1053
Size: 35 801 155 62
Fragments arranged by size:
801 155 62 35
SanDI GG'GwC_CC
Cuts at: 0 633 1053
Size: 633 420
SbfI CC_TGCA'GG
Cuts at: 0 991 1053
Size: 991 62
SfcI C'TryA_G
Cuts at: 0 31 111 832 970 987 1053
Size: 31 80 721 138 17 66
Fragments arranged by size:
721 138 80 66 31 17
SfoI GGC'GCC
Cuts at: 0 5 511 1053
Size: 5 506 542
Fragments arranged by size:
542 506 5
SgrAI Cr'CCGG_yG
Cuts at: 0 6 1053
Size: 6 1047
SpeI A'CTAG_T
Cuts at: 0 231 1053
Size: 231 822
SphI G_CATG'C
Cuts at: 0 813 1053
Size: 813 240
StuI AGG'CCT
Cuts at: 0 614 1053
Size: 614 439
StyI C'CwwG_G
Cuts at: 0 144 419 750 849 1031 1053
Size: 144 275 331 99 182 22
Fragments arranged by size:
331 275 182 144 99 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 1053
Size: 226 827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 1053
Size: 471 582
Enzymes that do cut:
Acc65I AleI AlwNI ApaI AvaI BaeI BamHI BanI
BanII BbeI BglI Bme1580I BmrI BmtI BpmI Bpu10I
BsaI BsaHI BsaWI BseRI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI
MslI MspA1I NarI NheI NspI PasI PpuMI PspOMI
PstI SanDI SbfI SfcI SfoI SgrAI SpeI SphI
StuI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BplI BpuEI BsaAI BsaBI BsaXI
BseYI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MmeI MscI NaeI NcoI NdeI
NgoMIV NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SapI ScaI SexAI
SfiI SgfI SmaI SmlI SnaBI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI