(Linear) (Six-base) MAPSORT of: KIBB8199.seq  Check: 7241  from: 1  to: 618



KIBB8199.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     618 
   Size:        247     371
 
ApoI r'AATT_y

Cuts at:      0     585     618 
   Size:        585      33
 
AvaI C'yCGr_G

Cuts at:      0      35     113     618 
   Size:         35      78     505

  Fragments arranged by size: 

                 505      78      35
 
BglI GCCn_nnn'nGGC

Cuts at:      0      71     618 
   Size:         71     547
 
BglII A'GATC_T

Cuts at:      0     573     618 
   Size:        573      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     618 
   Size:        566      52
 
Bpu10I CC'TnA_GC

Cuts at:      0      23     618 
   Size:         23     595
 
BseYI C'CCAG_C

Cuts at:      0     442     618 
   Size:        442     176
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     409     618 
   Size:        409     209
 
BspHI T'CATG_A

Cuts at:      0     146     618 
   Size:        146     472
 
BsrBI CCG'CTC

Cuts at:      0      22     618 
   Size:         22     596
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     538     618 
   Size:        538      80
 
BstYI r'GATC_y

Cuts at:      0     573     618 
   Size:        573      45
 
EaeI y'GGCC_r

Cuts at:      0     494     618 
   Size:        494     124
 
EarI CTCTTCn'nnn_

Cuts at:      0     305     618 
   Size:        305     313
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     122     618 
   Size:        122     496
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     566     618 
   Size:        247     319      52

  Fragments arranged by size: 

                 319     247      52
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     553     618 
   Size:        553      65
 
FspI TGC'GCA

Cuts at:      0     187     618 
   Size:        187     431
 
FspAI rTGC'GCAy

Cuts at:      0     187     618 
   Size:        187     431
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      72     104     618 
   Size:         72      32     514

  Fragments arranged by size: 

                 514      72      32
 
MspA1I CmG'CkG

Cuts at:      0     426     618 
   Size:        426     192
 
PasI CC'CwG_GG

Cuts at:      0      67     618 
   Size:         67     551
 
PstI C_TGCA'G

Cuts at:      0     176     618 
   Size:        176     442
 
PvuII CAG'CTG

Cuts at:      0     426     618 
   Size:        426     192
 
SfcI C'TryA_G

Cuts at:      0     172     618 
   Size:        172     446
 
SpeI A'CTAG_T

Cuts at:      0     478     618 
   Size:        478     140
 
StyI C'CwwG_G

Cuts at:      0     333     618 
   Size:        333     285
 
Tth111I GACn'n_nGTC

Cuts at:      0      84     618 
   Size:         84     534
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     516     618 
   Size:        516     102
 
XmnI GAAnn'nnTTC

Cuts at:      0     391     618 
   Size:        391     227
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI     BglI    BglII     BpmI   Bpu10I    BseYI
    BsmBI    BspHI    BsrBI   BstAPI    BstYI     EaeI     EarI     EciI
  Eco57MI     FalI     FspI    FspAI    Hin4I   MspA1I     PasI     PstI
    PvuII     SfcI     SpeI     StyI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspEI    BspMI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI     XbaI     XhoI     XmaI     ZraI