(Linear) (Six-base) MAPSORT of: KIBB8197.seq  Check: 5647  from: 1  to: 495



KIBB8197.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AccI GT'mk_AC

Cuts at:      0      35     495 
   Size:         35     460
 
AfeI AGC'GCT

Cuts at:      0     430     495 
   Size:        430      65
 
ApoI r'AATT_y

Cuts at:      0     102     244     495 
   Size:        102     142     251

  Fragments arranged by size: 

                 251     142     102
 
BanII G_rGCy'C

Cuts at:      0      47     495 
   Size:         47     448
 
BglI GCCn_nnn'nGGC

Cuts at:      0     212     495 
   Size:        212     283
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     324     495 
   Size:        324     171
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     495 
   Size:        143     352
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     327     495 
   Size:        327     168
 
BsiHKAI G_wGCw'C

Cuts at:      0     114     121     495 
   Size:        114       7     374

  Fragments arranged by size: 

                 374     114       7
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      63     495 
   Size:         63     432
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     114     121     495 
   Size:         47      67       7     374

  Fragments arranged by size: 

                 374      67      47       7
 
BsrDI GCAATG_nn'

Cuts at:      0      26     470     495 
   Size:         26     444      25

  Fragments arranged by size: 

                 444      26      25
 
BtgI C'CryG_G

Cuts at:      0     296     422     495 
   Size:        296     126      73

  Fragments arranged by size: 

                 296     126      73
 
BtsI GCAGTG_nn'

Cuts at:      0     344     495 
   Size:        344     151
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      31     495 
   Size:         31     464
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     495 
   Size:        143     352
 
EcoO109I rG'GnC_Cy

Cuts at:      0      84     280     495 
   Size:         84     196     215

  Fragments arranged by size: 

                 215     196      84
 
HaeII r_GCGC'y

Cuts at:      0     432     495 
   Size:        432      63
 
HincII GTy'rAC

Cuts at:      0       6      36     495 
   Size:          6      30     459

  Fragments arranged by size: 

                 459      30       6
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     495 
   Size:        344     151
 
NcoI C'CATG_G

Cuts at:      0     296     422     495 
   Size:        296     126      73

  Fragments arranged by size: 

                 296     126      73
 
NspI r_CATG'y

Cuts at:      0     379     495 
   Size:        379     116
 
PfoI T'CCnGG_A

Cuts at:      0     439     495 
   Size:        439      56
 
PpuMI rG'GwC_Cy

Cuts at:      0      84     280     495 
   Size:         84     196     215

  Fragments arranged by size: 

                 215     196      84
 
PsiI TTA'TAA

Cuts at:      0     143     495 
   Size:        143     352
 
SalI G'TCGA_C

Cuts at:      0      34     495 
   Size:         34     461
 
SspI AAT'ATT

Cuts at:      0     411     495 
   Size:        411      84
 
StyI C'CwwG_G

Cuts at:      0      47     296     422     495 
   Size:         47     249     126      73

  Fragments arranged by size: 

                 249     126      73      47
 
Tth111I GACn'n_nGTC

Cuts at:      0      82     495 
   Size:         82     413
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     214     495 
   Size:        214     281
 
XmnI GAAnn'nnTTC

Cuts at:      0     104     495 
   Size:        104     391
 

 Enzymes that do cut:

     AccI     AfeI     ApoI    BanII     BglI     BmrI     BpmI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BsrDI     BtgI     BtsI     DrdI  Eco57MI
 EcoO109I    HaeII   HincII     MmeI     NcoI     NspI     PfoI    PpuMI
     PsiI     SalI     SspI     StyI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI
     TstI     XbaI     XhoI     XmaI     ZraI