Restriction map for
pF1KB8195
(Linear) (Six-base) MAPSORT of: KIBB8195.seq Check: 7735 from: 1 to: 939
KIBB8195.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 939
Size: 631 308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 226 939
Size: 226 713
AlwNI CAG_nnn'CTG
Cuts at: 0 42 114 171 222 939
Size: 42 72 57 51 717
Fragments arranged by size:
717 72 57 51 42
BanI G'GyrC_C
Cuts at: 0 784 939
Size: 784 155
BanII G_rGCy'C
Cuts at: 0 232 359 939
Size: 232 127 580
Fragments arranged by size:
580 232 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 149 939
Size: 149 790
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 276 939
Size: 276 663
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 492 577 939
Size: 136 356 85 362
Fragments arranged by size:
362 356 136 85
Bpu10I CC'TnA_GC
Cuts at: 0 83 883 939
Size: 83 800 56
Fragments arranged by size:
800 83 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 939
Size: 580 359
BsaAI yAC'GTr
Cuts at: 0 525 939
Size: 525 414
BsaHI Gr'CG_yC
Cuts at: 0 907 939
Size: 907 32
BsaWI w'CCGG_w
Cuts at: 0 896 939
Size: 896 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 939
Size: 109 30 800
Fragments arranged by size:
800 109 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 240 268 939
Size: 240 28 671
Fragments arranged by size:
671 240 28
BseYI C'CCAG_C
Cuts at: 0 363 628 842 910 939
Size: 363 265 214 68 29
Fragments arranged by size:
363 265 214 68 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 939
Size: 454 485
BsiHKAI G_wGCw'C
Cuts at: 0 359 939
Size: 359 580
Bsp1286I G_dGCh'C
Cuts at: 0 232 359 939
Size: 232 127 580
Fragments arranged by size:
580 232 127
BspEI T'CCGG_A
Cuts at: 0 896 939
Size: 896 43
BspHI T'CATG_A
Cuts at: 0 689 939
Size: 689 250
BsrBI CCG'CTC
Cuts at: 0 447 939
Size: 447 492
BsrFI r'CCGG_y
Cuts at: 0 664 939
Size: 664 275
BstBI TT'CG_AA
Cuts at: 0 703 939
Size: 703 236
BstYI r'GATC_y
Cuts at: 0 893 939
Size: 893 46
Bsu36I CC'TnA_GG
Cuts at: 0 223 939
Size: 223 716
BtgI C'CryG_G
Cuts at: 0 197 533 635 939
Size: 197 336 102 304
Fragments arranged by size:
336 304 197 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 645 939
Size: 645 294
DrdI GACnn_nn'nnGTC
Cuts at: 0 585 939
Size: 585 354
EaeI y'GGCC_r
Cuts at: 0 809 939
Size: 809 130
EcoICRI GAG'CTC
Cuts at: 0 357 939
Size: 357 582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 492 577 631 939
Size: 136 356 85 54 308
Fragments arranged by size:
356 308 136 85 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 486 820 939
Size: 486 334 119
Fragments arranged by size:
486 334 119
EcoO109I rG'GnC_Cy
Cuts at: 0 66 219 624 939
Size: 66 153 405 315
Fragments arranged by size:
405 315 153 66
HaeII r_GCGC'y
Cuts at: 0 18 279 505 917 939
Size: 18 261 226 412 22
Fragments arranged by size:
412 261 226 22 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 885 917 939
Size: 885 32 22
Fragments arranged by size:
885 32 22
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 883 939
Size: 614 269 56
Fragments arranged by size:
614 269 56
MscI TGG'CCA
Cuts at: 0 811 939
Size: 811 128
MslI CAynn'nnrTG
Cuts at: 0 694 939
Size: 694 245
NaeI GCC'GGC
Cuts at: 0 666 939
Size: 666 273
NgoMIV G'CCGG_C
Cuts at: 0 664 939
Size: 664 275
PflMI CCAn_nnn'nTGG
Cuts at: 0 114 939
Size: 114 825
PmlI CAC'GTG
Cuts at: 0 525 939
Size: 525 414
PpuMI rG'GwC_Cy
Cuts at: 0 219 939
Size: 219 720
SacI G_AGCT'C
Cuts at: 0 359 939
Size: 359 580
SfcI C'TryA_G
Cuts at: 0 402 471 939
Size: 402 69 468
Fragments arranged by size:
468 402 69
SmlI C'TyrA_G
Cuts at: 0 595 939
Size: 595 344
StuI AGG'CCT
Cuts at: 0 481 939
Size: 481 458
StyI C'CwwG_G
Cuts at: 0 770 939
Size: 770 169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 111 642 939
Size: 111 531 297
Fragments arranged by size:
531 297 111
Enzymes that do cut:
AcuI AlfI AlwNI BanI BanII BbsI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspHI BsrBI BsrFI BstBI
BstYI Bsu36I BtgI BtgZI DrdI EaeI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I MmeI MscI MslI NaeI
NgoMIV PflMI PmlI PpuMI SacI SfcI SmlI StuI
StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BsaI BsaBI BsiEI BsiWI BsmI
BsmBI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstZ17I BtsI ClaI CspCI DraI DraIII EagI
EarI EciI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MspA1I
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI