Restriction map for
pF1KB8194
(Linear) (Six-base) MAPSORT of: KIBB8194.seq Check: 5808 from: 1 to: 1641
KIBB8194.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AccI GT'mk_AC
Cuts at: 0 329 1641
Size: 329 1312
AclI AA'CG_TT
Cuts at: 0 517 1641
Size: 517 1124
AflIII A'CryG_T
Cuts at: 0 701 1641
Size: 701 940
ApoI r'AATT_y
Cuts at: 0 561 1229 1471 1641
Size: 561 668 242 170
Fragments arranged by size:
668 561 242 170
AvaI C'yCGr_G
Cuts at: 0 1079 1641
Size: 1079 562
BamHI G'GATC_C
Cuts at: 0 1052 1461 1641
Size: 1052 409 180
Fragments arranged by size:
1052 409 180
BanI G'GyrC_C
Cuts at: 0 1573 1641
Size: 1573 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 1325 1641
Size: 1325 316
BglII A'GATC_T
Cuts at: 0 21 1641
Size: 21 1620
BlpI GC'TnA_GC
Cuts at: 0 35 1641
Size: 35 1606
BmrI ACTGGGnnnn_n'
Cuts at: 0 366 1641
Size: 366 1275
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 414 1037 1641
Size: 414 623 604
Fragments arranged by size:
623 604 414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 1641
Size: 748 893
Bpu10I CC'TnA_GC
Cuts at: 0 1125 1641
Size: 1125 516
BsaWI w'CCGG_w
Cuts at: 0 829 1641
Size: 829 812
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 185 1641
Size: 185 1456
BseYI C'CCAG_C
Cuts at: 0 158 1295 1641
Size: 158 1137 346
Fragments arranged by size:
1137 346 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 760 1641
Size: 702 58 881
Fragments arranged by size:
881 702 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 872 1641
Size: 872 769
BsrDI GCAATG_nn'
Cuts at: 0 179 1641
Size: 179 1462
BssSI C'ACGA_G
Cuts at: 0 88 1063 1641
Size: 88 975 578
Fragments arranged by size:
975 578 88
BstBI TT'CG_AA
Cuts at: 0 558 1388 1641
Size: 558 830 253
Fragments arranged by size:
830 558 253
BstEII G'GTnAC_C
Cuts at: 0 1141 1641
Size: 1141 500
BstYI r'GATC_y
Cuts at: 0 21 1052 1461 1641
Size: 21 1031 409 180
Fragments arranged by size:
1031 409 180 21
BstZ17I GTA'TAC
Cuts at: 0 330 1641
Size: 330 1311
BtgI C'CryG_G
Cuts at: 0 1072 1641
Size: 1072 569
BtsI GCAGTG_nn'
Cuts at: 0 803 1641
Size: 803 838
ClaI AT'CG_AT
Cuts at: 0 107 1641
Size: 107 1534
DraI TTT'AAA
Cuts at: 0 837 1641
Size: 837 804
DraIII CAC_nnn'GTG
Cuts at: 0 641 789 1641
Size: 641 148 852
Fragments arranged by size:
852 641 148
EaeI y'GGCC_r
Cuts at: 0 818 1246 1641
Size: 818 428 395
Fragments arranged by size:
818 428 395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 1641
Size: 748 893
EcoRV GAT'ATC
Cuts at: 0 177 1562 1641
Size: 177 1385 79
Fragments arranged by size:
1385 177 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 562 594 1641
Size: 562 32 1047
Fragments arranged by size:
1047 562 32
HincII GTy'rAC
Cuts at: 0 707 1641
Size: 707 934
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1197 1641
Size: 617 580 444
Fragments arranged by size:
617 580 444
MscI TGG'CCA
Cuts at: 0 820 1641
Size: 820 821
MslI CAynn'nnrTG
Cuts at: 0 695 1430 1641
Size: 695 735 211
Fragments arranged by size:
735 695 211
MspA1I CmG'CkG
Cuts at: 0 136 1641
Size: 136 1505
NcoI C'CATG_G
Cuts at: 0 1072 1641
Size: 1072 569
NdeI CA'TA_TG
Cuts at: 0 851 1641
Size: 851 790
NspI r_CATG'y
Cuts at: 0 705 1429 1497 1641
Size: 705 724 68 144
Fragments arranged by size:
724 705 144 68
PciI A'CATG_T
Cuts at: 0 701 1641
Size: 701 940
PspXI vC'TCGA_Gb
Cuts at: 0 1079 1641
Size: 1079 562
PvuII CAG'CTG
Cuts at: 0 136 1641
Size: 136 1505
SexAI A'CCwGG_T
Cuts at: 0 1031 1253 1641
Size: 1031 222 388
Fragments arranged by size:
1031 388 222
SfcI C'TryA_G
Cuts at: 0 53 436 1602 1641
Size: 53 383 1166 39
Fragments arranged by size:
1166 383 53 39
SmlI C'TyrA_G
Cuts at: 0 1079 1641
Size: 1079 562
SphI G_CATG'C
Cuts at: 0 1497 1641
Size: 1497 144
SspI AAT'ATT
Cuts at: 0 600 1641
Size: 600 1041
StyI C'CwwG_G
Cuts at: 0 1072 1641
Size: 1072 569
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 385 499 1384 1641
Size: 385 114 885 257
Fragments arranged by size:
885 385 257 114
TatI w'GTAC_w
Cuts at: 0 1092 1641
Size: 1092 549
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 379 1641
Size: 379 1262
XhoI C'TCGA_G
Cuts at: 0 1079 1641
Size: 1079 562
Enzymes that do cut:
AccI AclI AflIII ApoI AvaI BamHI BanI BbsI
BglII BlpI BmrI BplI BpmI Bpu10I BsaWI BseRI
BseYI BsgI BspMI BsrDI BssSI BstBI BstEII BstYI
BstZ17I BtgI BtsI ClaI DraI DraIII EaeI Eco57MI
EcoRV Hin4I HincII MmeI MscI MslI MspA1I NcoI
NdeI NspI PciI PspXI PvuII SexAI SfcI SmlI
SphI SspI StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI Bme1580I BmgBI BmtI BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BsrGI BssHII
BstAPI BstXI Bsu36I BtgZI CspCI DrdI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TstI
Tth111I XbaI XmaI XmnI ZraI