(Linear) (Six-base) MAPSORT of: KIBB8193.seq  Check: 7385  from: 1  to: 357



KIBB8193.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

Acc65I G'GTAC_C

Cuts at:      0     162     357 
   Size:        162     195
 
AlwNI CAG_nnn'CTG

Cuts at:      0     265     357 
   Size:        265      92
 
ApaI G_GGCC'C

Cuts at:      0      94     357 
   Size:         94     263
 
AvaI C'yCGr_G

Cuts at:      0      74     174     357 
   Size:         74     100     183

  Fragments arranged by size: 

                 183     100      74
 
BanI G'GyrC_C

Cuts at:      0     162     357 
   Size:        162     195
 
BanII G_rGCy'C

Cuts at:      0      16      94     311     357 
   Size:         16      78     217      46

  Fragments arranged by size: 

                 217      78      46      16
 
BbvCI CC'TCA_GC

Cuts at:      0     101     357 
   Size:        101     256
 
BlpI GC'TnA_GC

Cuts at:      0      10     112     190     357 
   Size:         10     102      78     167

  Fragments arranged by size: 

                 167     102      78      10
 
Bme1580I G_kGCm'C

Cuts at:      0      94     357 
   Size:         94     263
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     200     357 
   Size:        200     157
 
Bpu10I CC'TnA_GC

Cuts at:      0     101     357 
   Size:        101     256
 
BsaWI w'CCGG_w

Cuts at:      0     315     357 
   Size:        315      42
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     127     157     357 
   Size:        127      30     200

  Fragments arranged by size: 

                 200     127      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     357 
   Size:        118     239
 
BsiHKAI G_wGCw'C

Cuts at:      0     311     357 
   Size:        311      46
 
Bsp1286I G_dGCh'C

Cuts at:      0      16      94     311     357 
   Size:         16      78     217      46

  Fragments arranged by size: 

                 217      78      46      16
 
BspEI T'CCGG_A

Cuts at:      0     315     357 
   Size:        315      42
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     231     245     357 
   Size:        231      14     112

  Fragments arranged by size: 

                 231     112      14
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      54     357 
   Size:         54     303
 
BtsI GCAGTG_nn'

Cuts at:      0     135     357 
   Size:        135     222
 
EaeI y'GGCC_r

Cuts at:      0      65     251     357 
   Size:         65     186     106

  Fragments arranged by size: 

                 186     106      65
 
EcoICRI GAG'CTC

Cuts at:      0     309     357 
   Size:        309      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0      90     357 
   Size:         90     267
 
HaeII r_GCGC'y

Cuts at:      0      10     357 
   Size:         10     347
 
KpnI G_GTAC'C

Cuts at:      0     166     357 
   Size:        166     191
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     356     357 
   Size:         32     324       1

  Fragments arranged by size: 

                 324      32       1
 
MscI TGG'CCA

Cuts at:      0      67     357 
   Size:         67     290
 
MspA1I CmG'CkG

Cuts at:      0     100     111     357 
   Size:        100      11     246

  Fragments arranged by size: 

                 246     100      11
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     265     357 
   Size:        265      92
 
PspOMI G'GGCC_C

Cuts at:      0      90     357 
   Size:         90     267
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     124     156     357 
   Size:        124      32     201

  Fragments arranged by size: 

                 201     124      32
 
PstI C_TGCA'G

Cuts at:      0     242     357 
   Size:        242     115
 
PvuII CAG'CTG

Cuts at:      0     100     357 
   Size:        100     257
 
SacI G_AGCT'C

Cuts at:      0     311     357 
   Size:        311      46
 
SbfI CC_TGCA'GG

Cuts at:      0     242     357 
   Size:        242     115
 
SfcI C'TryA_G

Cuts at:      0     208     238     357 
   Size:        208      30     119

  Fragments arranged by size: 

                 208     119      30
 
SmaI CCC'GGG

Cuts at:      0      76     357 
   Size:         76     281
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     127     159     357 
   Size:        127      32     198

  Fragments arranged by size: 

                 198     127      32
 
XmaI C'CCGG_G

Cuts at:      0      74     357 
   Size:         74     283
 

 Enzymes that do cut:

   Acc65I    AlwNI     ApaI     AvaI     BanI    BanII    BbvCI     BlpI
 Bme1580I     BplI   Bpu10I    BsaWI    BsaXI    BseRI  BsiHKAI Bsp1286I
    BspEI    BspMI    BtgZI     BtsI     EaeI  EcoICRI EcoO109I    HaeII
     KpnI     MmeI     MscI   MspA1I    PflMI   PspOMI     PsrI     PstI
    PvuII     SacI     SbfI     SfcI     SmaI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI