(Linear) (Six-base) MAPSORT of: KIBB8192.seq  Check: 2211  from: 1  to: 672



KIBB8192.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     672 
   Size:        317     355
 
AflIII A'CryG_T

Cuts at:      0     539     672 
   Size:        539     133
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     424     456     672 
   Size:        424      32     216

  Fragments arranged by size: 

                 424     216      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      57     672 
   Size:         57     615
 
ApoI r'AATT_y

Cuts at:      0      85     125     672 
   Size:         85      40     547

  Fragments arranged by size: 

                 547      85      40
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     530     672 
   Size:        530     142
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     430     672 
   Size:        430     242
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59      79     672 
   Size:         59      20     593

  Fragments arranged by size: 

                 593      59      20
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     633     672 
   Size:        241     392      39

  Fragments arranged by size: 

                 392     241      39
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     375     672 
   Size:        375     297
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     282     636     672 
   Size:        282     354      36

  Fragments arranged by size: 

                 354     282      36
 
BsiHKAI G_wGCw'C

Cuts at:      0     351     672 
   Size:        351     321
 
Bsp1286I G_dGCh'C

Cuts at:      0     351     672 
   Size:        351     321
 
BspHI T'CATG_A

Cuts at:      0     132     672 
   Size:        132     540
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     337     672 
   Size:        337     335
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     359     672 
   Size:        359     313
 
BtgI C'CryG_G

Cuts at:      0     523     672 
   Size:        523     149
 
EaeI y'GGCC_r

Cuts at:      0     459     672 
   Size:        459     213
 
EarI CTCTTCn'nnn_

Cuts at:      0     160     672 
   Size:        160     512
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59      79     317     672 
   Size:         59      20     238     355

  Fragments arranged by size: 

                 355     238      59      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     372     672 
   Size:         54     318     300

  Fragments arranged by size: 

                 318     300      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     420     452     619     651     672 
   Size:        420      32     167      32      21

  Fragments arranged by size: 

                 420     167      32      32      21
 
HincII GTy'rAC

Cuts at:      0     638     672 
   Size:        638      34
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     672 
   Size:        566     106
 
MscI TGG'CCA

Cuts at:      0     461     672 
   Size:        461     211
 
MslI CAynn'nnrTG

Cuts at:      0      77     544     572     672 
   Size:         77     467      28     100

  Fragments arranged by size: 

                 467     100      77      28
 
MspA1I CmG'CkG

Cuts at:      0     232     672 
   Size:        232     440
 
NcoI C'CATG_G

Cuts at:      0     523     672 
   Size:        523     149
 
NspI r_CATG'y

Cuts at:      0     543     672 
   Size:        543     129
 
PciI A'CATG_T

Cuts at:      0     539     672 
   Size:        539     133
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      57     672 
   Size:         57     615
 
PfoI T'CCnGG_A

Cuts at:      0      56     672 
   Size:         56     616
 
PpuMI rG'GwC_Cy

Cuts at:      0      54     372     672 
   Size:         54     318     300

  Fragments arranged by size: 

                 318     300      54
 
PvuII CAG'CTG

Cuts at:      0     232     672 
   Size:        232     440
 
SanDI GG'GwC_CC

Cuts at:      0     372     672 
   Size:        372     300
 
SexAI A'CCwGG_T

Cuts at:      0      50     672 
   Size:         50     622
 
SmlI C'TyrA_G

Cuts at:      0     256     648     672 
   Size:        256     392      24

  Fragments arranged by size: 

                 392     256      24
 
StyI C'CwwG_G

Cuts at:      0     523     672 
   Size:        523     149
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     521     672 
   Size:        521     151
 

 Enzymes that do cut:

     AcuI   AflIII     AloI    AlwNI     ApoI    BciVI     BmrI     BpmI
    BpuEI     BsaI    BseRI  BsiHKAI Bsp1286I    BspHI   BstAPI    BstXI
     BtgI     EaeI     EarI  Eco57MI EcoO109I    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NcoI     NspI     PciI    PflMI     PfoI
    PpuMI    PvuII    SanDI    SexAI     SmlI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI