(Linear) (Six-base) MAPSORT of: KIBB8190.seq  Check: 8392  from: 1  to: 447



KIBB8190.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AccI GT'mk_AC

Cuts at:      0      41     447 
   Size:         41     406
 
AflII C'TTAA_G

Cuts at:      0     153     447 
   Size:        153     294
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     447 
   Size:        272     175
 
BsaBI GATnn'nnATC

Cuts at:      0     101     447 
   Size:        101     346
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     377     447 
   Size:        377      70
 
BsrDI GCAATG_nn'

Cuts at:      0     374     447 
   Size:        374      73
 
BstYI r'GATC_y

Cuts at:      0     102     283     447 
   Size:        102     181     164

  Fragments arranged by size: 

                 181     164     102
 
EarI CTCTTCn'nnn_

Cuts at:      0     150     447 
   Size:        150     297
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     447 
   Size:        272     175
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     268     300     447 
   Size:        268      32     147

  Fragments arranged by size: 

                 268     147      32
 
HindIII A'AGCT_T

Cuts at:      0     312     379     447 
   Size:        312      67      68

  Fragments arranged by size: 

                 312      68      67
 
NdeI CA'TA_TG

Cuts at:      0     266     447 
   Size:        266     181
 
PstI C_TGCA'G

Cuts at:      0     119     201     447 
   Size:        119      82     246

  Fragments arranged by size: 

                 246     119      82
 
ScaI AGT'ACT

Cuts at:      0      60     447 
   Size:         60     387
 
SexAI A'CCwGG_T

Cuts at:      0     176     447 
   Size:        176     271
 
SfcI C'TryA_G

Cuts at:      0     115     197     447 
   Size:        115      82     250

  Fragments arranged by size: 

                 250     115      82
 
SmlI C'TyrA_G

Cuts at:      0     153     447 
   Size:        153     294
 
SspI AAT'ATT

Cuts at:      0     414     447 
   Size:        414      33
 
TatI w'GTAC_w

Cuts at:      0      58     447 
   Size:         58     389
 

 Enzymes that do cut:

     AccI    AflII     BpmI    BsaBI    BseRI    BsrDI    BstYI     EarI
  Eco57MI    Hin4I  HindIII     NdeI     PstI     ScaI    SexAI     SfcI
     SmlI     SspI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI