Restriction map for
pF1KB8187
(Linear) (Six-base) MAPSORT of: KIBB8187.seq Check: 6561 from: 1 to: 1356
KIBB8187.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 789 1065 1356
Size: 789 276 291
Fragments arranged by size:
789 291 276
Acc65I G'GTAC_C
Cuts at: 0 1167 1356
Size: 1167 189
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 1115 1356
Size: 685 430 241
Fragments arranged by size:
685 430 241
AflIII A'CryG_T
Cuts at: 0 1121 1356
Size: 1121 235
AlwNI CAG_nnn'CTG
Cuts at: 0 383 1356
Size: 383 973
ApaI G_GGCC'C
Cuts at: 0 372 1356
Size: 372 984
ApoI r'AATT_y
Cuts at: 0 1312 1356
Size: 1312 44
BamHI G'GATC_C
Cuts at: 0 823 1356
Size: 823 533
BanI G'GyrC_C
Cuts at: 0 423 763 850 1167 1356
Size: 423 340 87 317 189
Fragments arranged by size:
423 340 317 189 87
BanII G_rGCy'C
Cuts at: 0 372 938 1356
Size: 372 566 418
Fragments arranged by size:
566 418 372
BbsI GAAGACnn'nnnn_
Cuts at: 0 1103 1298 1356
Size: 1103 195 58
Fragments arranged by size:
1103 195 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 21 55 840 874 1356
Size: 21 34 785 34 482
Fragments arranged by size:
785 482 34 34 21
BclI T'GATC_A
Cuts at: 0 158 1356
Size: 158 1198
BglI GCCn_nnn'nGGC
Cuts at: 0 29 131 1356
Size: 29 102 1225
Fragments arranged by size:
1225 102 29
BglII A'GATC_T
Cuts at: 0 1277 1356
Size: 1277 79
BlpI GC'TnA_GC
Cuts at: 0 1113 1356
Size: 1113 243
Bme1580I G_kGCm'C
Cuts at: 0 174 308 372 1356
Size: 174 134 64 984
Fragments arranged by size:
984 174 134 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 126 748 1356
Size: 126 622 608
Fragments arranged by size:
622 608 126
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 153 457 624 1356
Size: 153 304 167 732
Fragments arranged by size:
732 304 167 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 699 1356
Size: 632 67 657
Fragments arranged by size:
657 632 67
Bpu10I CC'TnA_GC
Cuts at: 0 724 1356
Size: 724 632
BsaBI GATnn'nnATC
Cuts at: 0 157 1356
Size: 157 1199
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 776 1356
Size: 776 580
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1356
Size: 295 1061
BsiHKAI G_wGCw'C
Cuts at: 0 752 1356
Size: 752 604
Bsp1286I G_dGCh'C
Cuts at: 0 174 308 372 752 938 1356
Size: 174 134 64 380 186 418
Fragments arranged by size:
418 380 186 174 134 64
BspHI T'CATG_A
Cuts at: 0 1271 1356
Size: 1271 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 789 1065 1356
Size: 789 276 291
Fragments arranged by size:
789 291 276
BsrBI CCG'CTC
Cuts at: 0 137 450 1356
Size: 137 313 906
Fragments arranged by size:
906 313 137
BsrDI GCAATG_nn'
Cuts at: 0 648 1356
Size: 648 708
BsrGI T'GTAC_A
Cuts at: 0 246 1356
Size: 246 1110
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1039 1356
Size: 1039 317
BstYI r'GATC_y
Cuts at: 0 823 1277 1356
Size: 823 454 79
Fragments arranged by size:
823 454 79
BtgI C'CryG_G
Cuts at: 0 192 1356
Size: 192 1164
BtsI GCAGTG_nn'
Cuts at: 0 290 1142 1356
Size: 290 852 214
Fragments arranged by size:
852 290 214
DraIII CAC_nnn'GTG
Cuts at: 0 20 1356
Size: 20 1336
EarI CTCTTCn'nnn_
Cuts at: 0 685 704 1356
Size: 685 19 652
Fragments arranged by size:
685 652 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 685 699 1115 1356
Size: 632 53 14 416 241
Fragments arranged by size:
632 416 241 53 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 82 1356
Size: 82 1274
EcoO109I rG'GnC_Cy
Cuts at: 0 351 368 369 1356
Size: 351 17 1 987
Fragments arranged by size:
987 351 17 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 941 973 1356
Size: 941 32 383
Fragments arranged by size:
941 383 32
KpnI G_GTAC'C
Cuts at: 0 1171 1356
Size: 1171 185
MslI CAynn'nnrTG
Cuts at: 0 299 652 743 1324 1356
Size: 299 353 91 581 32
Fragments arranged by size:
581 353 299 91 32
MspA1I CmG'CkG
Cuts at: 0 632 1356
Size: 632 724
NdeI CA'TA_TG
Cuts at: 0 819 1356
Size: 819 537
NspI r_CATG'y
Cuts at: 0 955 1050 1125 1356
Size: 955 95 75 231
Fragments arranged by size:
955 231 95 75
PciI A'CATG_T
Cuts at: 0 1121 1356
Size: 1121 235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 1356
Size: 538 32 786
Fragments arranged by size:
786 538 32
PpuMI rG'GwC_Cy
Cuts at: 0 351 1356
Size: 351 1005
PspOMI G'GGCC_C
Cuts at: 0 368 1356
Size: 368 988
PstI C_TGCA'G
Cuts at: 0 1085 1356
Size: 1085 271
SapI GCTCTTCn'nnn_
Cuts at: 0 685 1356
Size: 685 671
SexAI A'CCwGG_T
Cuts at: 0 353 1242 1356
Size: 353 889 114
Fragments arranged by size:
889 353 114
SfcI C'TryA_G
Cuts at: 0 1081 1356
Size: 1081 275
SmlI C'TyrA_G
Cuts at: 0 1336 1356
Size: 1336 20
StuI AGG'CCT
Cuts at: 0 1223 1356
Size: 1223 133
StyI C'CwwG_G
Cuts at: 0 419 1356
Size: 419 937
TatI w'GTAC_w
Cuts at: 0 246 263 682 837 1356
Size: 246 17 419 155 519
Fragments arranged by size:
519 419 246 155 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1193 1225 1356
Size: 1193 32 131
Fragments arranged by size:
1193 131 32
Tth111I GACn'n_nGTC
Cuts at: 0 649 1356
Size: 649 707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 861 1356
Size: 861 495
XmnI GAAnn'nnTTC
Cuts at: 0 1223 1356
Size: 1223 133
Enzymes that do cut:
AarI Acc65I AcuI AflIII AlwNI ApaI ApoI BamHI
BanI BanII BbsI BcgI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaBI BseRI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstXI
BstYI BtgI BtsI DraIII EarI Eco57MI EcoNI EcoO109I
Hin4I KpnI MslI MspA1I NdeI NspI PciI PpiI
PpuMI PspOMI PstI SapI SexAI SfcI SmlI StuI
StyI TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbvCI BciVI BfrBI BmgBI BmtI BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EaeI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII XbaI XhoI XmaI ZraI