Restriction map for
pF1KB8186
(Linear) (Six-base) MAPSORT of: KIBB8186.seq Check: 2959 from: 1 to: 909
KIBB8186.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AgeI A'CCGG_T
Cuts at: 0 82 909
Size: 82 827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 763 909
Size: 763 146
AlwNI CAG_nnn'CTG
Cuts at: 0 771 909
Size: 771 138
ApaLI G'TGCA_C
Cuts at: 0 636 909
Size: 636 273
ApoI r'AATT_y
Cuts at: 0 40 187 260 812 909
Size: 40 147 73 552 97
Fragments arranged by size:
552 147 97 73 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 259 909
Size: 259 650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 132 166 425 459 909
Size: 132 34 259 34 450
Fragments arranged by size:
450 259 132 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 140 909
Size: 140 769
Bme1580I G_kGCm'C
Cuts at: 0 389 640 909
Size: 389 251 269
Fragments arranged by size:
389 269 251
BmrI ACTGGGnnnn_n'
Cuts at: 0 383 592 703 909
Size: 383 209 111 206
Fragments arranged by size:
383 209 206 111
Bpu10I CC'TnA_GC
Cuts at: 0 563 909
Size: 563 346
BsaAI yAC'GTr
Cuts at: 0 117 909
Size: 117 792
BsaWI w'CCGG_w
Cuts at: 0 82 909
Size: 82 827
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 422 443 909
Size: 37 385 21 466
Fragments arranged by size:
466 385 37 21
BseYI C'CCAG_C
Cuts at: 0 465 909
Size: 465 444
BsiHKAI G_wGCw'C
Cuts at: 0 640 909
Size: 640 269
BsiWI C'GTAC_G
Cuts at: 0 833 909
Size: 833 76
Bsp1286I G_dGCh'C
Cuts at: 0 389 640 909
Size: 389 251 269
Fragments arranged by size:
389 269 251
BspHI T'CATG_A
Cuts at: 0 420 909
Size: 420 489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 770 909
Size: 770 139
BsrFI r'CCGG_y
Cuts at: 0 82 909
Size: 82 827
BstAPI GCAn_nnn'nTGC
Cuts at: 0 521 771 909
Size: 521 250 138
Fragments arranged by size:
521 250 138
BstYI r'GATC_y
Cuts at: 0 101 245 909
Size: 101 144 664
Fragments arranged by size:
664 144 101
BtgI C'CryG_G
Cuts at: 0 6 800 909
Size: 6 794 109
Fragments arranged by size:
794 109 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 395 909
Size: 395 514
EaeI y'GGCC_r
Cuts at: 0 776 909
Size: 776 133
HindIII A'AGCT_T
Cuts at: 0 454 909
Size: 454 455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 909
Size: 651 258
MslI CAynn'nnrTG
Cuts at: 0 163 909
Size: 163 746
MspA1I CmG'CkG
Cuts at: 0 8 32 909
Size: 8 24 877
Fragments arranged by size:
877 24 8
NcoI C'CATG_G
Cuts at: 0 800 909
Size: 800 109
NdeI CA'TA_TG
Cuts at: 0 300 909
Size: 300 609
PfoI T'CCnGG_A
Cuts at: 0 323 909
Size: 323 586
PsiI TTA'TAA
Cuts at: 0 532 909
Size: 532 377
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 835 867 909
Size: 835 32 42
Fragments arranged by size:
835 42 32
PstI C_TGCA'G
Cuts at: 0 448 722 752 767 909
Size: 448 274 30 15 142
Fragments arranged by size:
448 274 142 30 15
PvuII CAG'CTG
Cuts at: 0 32 909
Size: 32 877
SacII CC_GC'GG
Cuts at: 0 9 909
Size: 9 900
SfcI C'TryA_G
Cuts at: 0 444 718 748 763 909
Size: 444 274 30 15 146
Fragments arranged by size:
444 274 146 30 15
SnaBI TAC'GTA
Cuts at: 0 117 909
Size: 117 792
SspI AAT'ATT
Cuts at: 0 122 909
Size: 122 787
StyI C'CwwG_G
Cuts at: 0 17 584 800 909
Size: 17 567 216 109
Fragments arranged by size:
567 216 109 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 224 909
Size: 224 685
TatI w'GTAC_w
Cuts at: 0 303 332 909
Size: 303 29 577
Fragments arranged by size:
577 303 29
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 798 909
Size: 798 111
XmnI GAAnn'nnTTC
Cuts at: 0 40 909
Size: 40 869
Enzymes that do cut:
AgeI AlfI AlwNI ApaLI ApoI BbsI BcgI BglI
Bme1580I BmrI Bpu10I BsaAI BsaWI BseRI BseYI BsiHKAI
BsiWI Bsp1286I BspHI BspMI BsrFI BstAPI BstYI BtgI
BtgZI EaeI HindIII MmeI MslI MspA1I NcoI NdeI
PfoI PsiI PsrI PstI PvuII SacII SfcI SnaBI
SspI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
BpmI BpuEI BsaI BsaBI BsaHI BsaXI BsgI BsiEI
BsmI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI StuI SwaI
TstI Tth111I XbaI XhoI XmaI ZraI