Restriction map for
pF1KB8175
(Linear) (Six-base) MAPSORT of: KIBB8175.seq Check: 2807 from: 1 to: 1503
KIBB8175.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AatII G_ACGT'C
Cuts at: 0 62 1503
Size: 62 1441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 475 562 1385 1503
Size: 361 114 87 823 118
Fragments arranged by size:
823 361 118 114 87
ApaI G_GGCC'C
Cuts at: 0 1256 1503
Size: 1256 247
ApoI r'AATT_y
Cuts at: 0 247 277 325 1503
Size: 247 30 48 1178
Fragments arranged by size:
1178 247 48 30
AvaI C'yCGr_G
Cuts at: 0 128 347 1000 1503
Size: 128 219 653 503
Fragments arranged by size:
653 503 219 128
BanI G'GyrC_C
Cuts at: 0 925 1503
Size: 925 578
BanII G_rGCy'C
Cuts at: 0 215 726 846 1256 1503
Size: 215 511 120 410 247
Fragments arranged by size:
511 410 247 215 120
BbsI GAAGACnn'nnnn_
Cuts at: 0 1445 1474 1503
Size: 1445 29 29
Fragments arranged by size:
1445 29 29
BclI T'GATC_A
Cuts at: 0 117 1503
Size: 117 1386
BglII A'GATC_T
Cuts at: 0 302 977 1503
Size: 302 675 526
Fragments arranged by size:
675 526 302
Bme1580I G_kGCm'C
Cuts at: 0 708 928 1256 1503
Size: 708 220 328 247
Fragments arranged by size:
708 328 247 220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 740 898 1503
Size: 34 706 158 605
Fragments arranged by size:
706 605 158 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 424 589 1428 1503
Size: 247 177 165 839 75
Fragments arranged by size:
839 247 177 165 75
BsaBI GATnn'nnATC
Cuts at: 0 789 1503
Size: 789 714
BsaHI Gr'CG_yC
Cuts at: 0 59 1287 1503
Size: 59 1228 216
Fragments arranged by size:
1228 216 59
BsaWI w'CCGG_w
Cuts at: 0 284 1503
Size: 284 1219
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 223 738 772 1503
Size: 52 171 515 34 731
Fragments arranged by size:
731 515 171 52 34
BseYI C'CCAG_C
Cuts at: 0 1157 1503
Size: 1157 346
BsiHKAI G_wGCw'C
Cuts at: 0 215 1503
Size: 215 1288
BsmI GAATG_Cn'
Cuts at: 0 540 742 1503
Size: 540 202 761
Fragments arranged by size:
761 540 202
Bsp1286I G_dGCh'C
Cuts at: 0 215 708 726 846 928 1256 1503
Size: 215 493 18 120 82 328 247
Fragments arranged by size:
493 328 247 215 120 82 18
BspHI T'CATG_A
Cuts at: 0 33 1023 1503
Size: 33 990 480
Fragments arranged by size:
990 480 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1154 1503
Size: 1154 349
BsrDI GCAATG_nn'
Cuts at: 0 956 1503
Size: 956 547
BsrGI T'GTAC_A
Cuts at: 0 1032 1503
Size: 1032 471
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1157 1503
Size: 1157 346
BstYI r'GATC_y
Cuts at: 0 302 977 1479 1503
Size: 302 675 502 24
Fragments arranged by size:
675 502 302 24
Bsu36I CC'TnA_GG
Cuts at: 0 169 1503
Size: 169 1334
BtsI GCAGTG_nn'
Cuts at: 0 708 1325 1503
Size: 708 617 178
Fragments arranged by size:
708 617 178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 302 337 1503
Size: 302 35 1166
Fragments arranged by size:
1166 302 35
EaeI y'GGCC_r
Cuts at: 0 685 1503
Size: 685 818
EarI CTCTTCn'nnn_
Cuts at: 0 359 392 830 1055 1067 1076 1109 1503
Size: 359 33 438 225 12 9 33 394
Fragments arranged by size:
438 394 359 225 33 33 12 9
EcoICRI GAG'CTC
Cuts at: 0 213 1503
Size: 213 1290
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 361 475 562 740 898 1385 1503
Size: 34 327 114 87 178 158 487 118
Fragments arranged by size:
487 327 178 158 118 114 87 34
EcoO109I rG'GnC_Cy
Cuts at: 0 229 671 827 1252 1503
Size: 229 442 156 425 251
Fragments arranged by size:
442 425 251 229 156
EcoRI G'AATT_C
Cuts at: 0 247 277 1503
Size: 247 30 1226
Fragments arranged by size:
1226 247 30
HaeII r_GCGC'y
Cuts at: 0 30 1503
Size: 30 1473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1351 1383 1503
Size: 1351 32 120
Fragments arranged by size:
1351 120 32
HindIII A'AGCT_T
Cuts at: 0 1192 1503
Size: 1192 311
MslI CAynn'nnrTG
Cuts at: 0 296 1028 1295 1503
Size: 296 732 267 208
Fragments arranged by size:
732 296 267 208
MspA1I CmG'CkG
Cuts at: 0 24 1503
Size: 24 1479
PfoI T'CCnGG_A
Cuts at: 0 98 678 1503
Size: 98 580 825
Fragments arranged by size:
825 580 98
PpuMI rG'GwC_Cy
Cuts at: 0 229 671 827 1503
Size: 229 442 156 676
Fragments arranged by size:
676 442 229 156
PspOMI G'GGCC_C
Cuts at: 0 1252 1503
Size: 1252 251
SacI G_AGCT'C
Cuts at: 0 215 1503
Size: 215 1288
SanDI GG'GwC_CC
Cuts at: 0 827 1503
Size: 827 676
SapI GCTCTTCn'nnn_
Cuts at: 0 830 1055 1076 1503
Size: 830 225 21 427
Fragments arranged by size:
830 427 225 21
SmlI C'TyrA_G
Cuts at: 0 262 347 439 568 1443 1503
Size: 262 85 92 129 875 60
Fragments arranged by size:
875 262 129 92 85 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 308 1503
Size: 308 1195
TatI w'GTAC_w
Cuts at: 0 443 1032 1503
Size: 443 589 471
Fragments arranged by size:
589 471 443
XbaI T'CTAG_A
Cuts at: 0 966 1503
Size: 966 537
XhoI C'TCGA_G
Cuts at: 0 347 1503
Size: 347 1156
ZraI GAC'GTC
Cuts at: 0 60 1503
Size: 60 1443
Enzymes that do cut:
AatII AcuI ApaI ApoI AvaI BanI BanII BbsI
BclI BglII Bme1580I BpmI BpuEI BsaBI BsaHI BsaWI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrGI BstXI BstYI Bsu36I BtsI CspCI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HindIII MslI
MspA1I PfoI PpuMI PspOMI SacI SanDI SapI SmlI
TaqII TatI XbaI XhoI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaXI BsgI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgI BtgZI ClaI DraI DraIII DrdI EagI EciI
EcoNI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TstI Tth111I XcmI XmaI XmnI