Restriction map for
pF1KB8171
(Linear) (Six-base) MAPSORT of: KIBB8171.seq Check: 4363 from: 1 to: 1671
KIBB8171.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 214 1671
Size: 214 1457
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 415 461 498 1413 1506 1595 1671
Size: 334 81 46 37 915 93 89 76
Fragments arranged by size:
915 334 93 89 81 76 46 37
AflII C'TTAA_G
Cuts at: 0 606 1671
Size: 606 1065
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 957 958 989 990 1475 1507 1671
Size: 957 1 31 1 485 32 164
Fragments arranged by size:
957 485 164 32 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 62 270 573 844 1446 1671
Size: 62 208 303 271 602 225
Fragments arranged by size:
602 303 271 225 208 62
ApaLI G'TGCA_C
Cuts at: 0 345 577 1671
Size: 345 232 1094
Fragments arranged by size:
1094 345 232
ApoI r'AATT_y
Cuts at: 0 539 642 730 1169 1671
Size: 539 103 88 439 502
Fragments arranged by size:
539 502 439 103 88
AvaI C'yCGr_G
Cuts at: 0 1358 1671
Size: 1358 313
BanI G'GyrC_C
Cuts at: 0 1090 1671
Size: 1090 581
BbeI G_GCGC'C
Cuts at: 0 1094 1671
Size: 1094 577
BbsI GAAGACnn'nnnn_
Cuts at: 0 1154 1195 1671
Size: 1154 41 476
Fragments arranged by size:
1154 476 41
BciVI GTATCCnnnnn_n'
Cuts at: 0 1091 1671
Size: 1091 580
BfrBI ATG'CAT
Cuts at: 0 879 1671
Size: 879 792
Bme1580I G_kGCm'C
Cuts at: 0 349 581 1520 1671
Size: 349 232 939 151
Fragments arranged by size:
939 349 232 151
BmrI ACTGGGnnnn_n'
Cuts at: 0 52 211 705 1671
Size: 52 159 494 966
Fragments arranged by size:
966 494 159 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1088 1529 1671
Size: 1088 441 142
Fragments arranged by size:
1088 441 142
Bpu10I CC'TnA_GC
Cuts at: 0 1208 1671
Size: 1208 463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1671
Size: 133 1538
BsaI GGTCTCn'nnnn_
Cuts at: 0 653 1122 1671
Size: 653 469 549
Fragments arranged by size:
653 549 469
BsaHI Gr'CG_yC
Cuts at: 0 1091 1671
Size: 1091 580
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 685 715 1475 1505 1671
Size: 685 30 760 30 166
Fragments arranged by size:
760 685 166 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 773 1671
Size: 271 502 898
Fragments arranged by size:
898 502 271
BseYI C'CCAG_C
Cuts at: 0 612 1671
Size: 612 1059
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 802 1184 1671
Size: 283 519 382 487
Fragments arranged by size:
519 487 382 283
BsiHKAI G_wGCw'C
Cuts at: 0 349 581 1671
Size: 349 232 1090
Fragments arranged by size:
1090 349 232
BsmI GAATG_Cn'
Cuts at: 0 478 879 1671
Size: 478 401 792
Fragments arranged by size:
792 478 401
Bsp1286I G_dGCh'C
Cuts at: 0 349 581 1520 1671
Size: 349 232 939 151
Fragments arranged by size:
939 349 232 151
BspHI T'CATG_A
Cuts at: 0 1122 1671
Size: 1122 549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 214 1671
Size: 214 1457
BsrDI GCAATG_nn'
Cuts at: 0 462 770 1235 1671
Size: 462 308 465 436
Fragments arranged by size:
465 462 436 308
BsrFI r'CCGG_y
Cuts at: 0 671 1671
Size: 671 1000
BstAPI GCAn_nnn'nTGC
Cuts at: 0 270 344 484 576 1671
Size: 270 74 140 92 1095
Fragments arranged by size:
1095 270 140 92 74
BstEII G'GTnAC_C
Cuts at: 0 1472 1671
Size: 1472 199
BstXI CCAn_nnnn'nTGG
Cuts at: 0 42 1671
Size: 42 1629
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1283 1671
Size: 1283 388
BtsI GCAGTG_nn'
Cuts at: 0 175 790 941 1671
Size: 175 615 151 730
Fragments arranged by size:
730 615 175 151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1218 1253 1671
Size: 1218 35 418
Fragments arranged by size:
1218 418 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 139 1671
Size: 139 1532
EaeI y'GGCC_r
Cuts at: 0 354 1349 1671
Size: 354 995 322
Fragments arranged by size:
995 354 322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 415 461 498 1088 1413 1506 1529
Size: 334 81 46 37 590 325 93 23
Cuts at: 1529 1595 1671
Size: 66 76
Fragments arranged by size:
590 334 325 93 81 76 66 46
37 23
EcoO109I rG'GnC_Cy
Cuts at: 0 957 1671
Size: 957 714
EcoRI G'AATT_C
Cuts at: 0 730 1169 1671
Size: 730 439 502
Fragments arranged by size:
730 502 439
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 528 735 1671
Size: 528 207 936
Fragments arranged by size:
936 528 207
HaeII r_GCGC'y
Cuts at: 0 270 1094 1671
Size: 270 824 577
Fragments arranged by size:
824 577 270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 685 717 840 872 1490 1522
Size: 144 32 509 32 123 32 618 32
Cuts at: 1522 1671
Size: 149
Fragments arranged by size:
618 509 149 144 123 32 32 32
32
HincII GTy'rAC
Cuts at: 0 183 1671
Size: 183 1488
HindIII A'AGCT_T
Cuts at: 0 1019 1671
Size: 1019 652
KasI G'GCGC_C
Cuts at: 0 1090 1671
Size: 1090 581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1239 1671
Size: 1239 432
MscI TGG'CCA
Cuts at: 0 356 1671
Size: 356 1315
MslI CAynn'nnrTG
Cuts at: 0 430 1345 1405 1441 1671
Size: 430 915 60 36 230
Fragments arranged by size:
915 430 230 60 36
MspA1I CmG'CkG
Cuts at: 0 31 573 656 971 1549 1671
Size: 31 542 83 315 578 122
Fragments arranged by size:
578 542 315 122 83 31
NaeI GCC'GGC
Cuts at: 0 673 1671
Size: 673 998
NarI GG'CG_CC
Cuts at: 0 1091 1671
Size: 1091 580
NgoMIV G'CCGG_C
Cuts at: 0 671 1671
Size: 671 1000
NsiI A_TGCA'T
Cuts at: 0 881 1671
Size: 881 790
NspI r_CATG'y
Cuts at: 0 1440 1671
Size: 1440 231
PflMI CCAn_nnn'nTGG
Cuts at: 0 1593 1671
Size: 1593 78
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1475 1507 1671
Size: 1475 32 164
Fragments arranged by size:
1475 164 32
PpuMI rG'GwC_Cy
Cuts at: 0 957 1671
Size: 957 714
PvuII CAG'CTG
Cuts at: 0 573 971 1549 1671
Size: 573 398 578 122
Fragments arranged by size:
578 573 398 122
SanDI GG'GwC_CC
Cuts at: 0 957 1671
Size: 957 714
SfcI C'TryA_G
Cuts at: 0 932 1671
Size: 932 739
SfoI GGC'GCC
Cuts at: 0 1092 1671
Size: 1092 579
SmaI CCC'GGG
Cuts at: 0 1360 1671
Size: 1360 311
SmlI C'TyrA_G
Cuts at: 0 148 606 1671
Size: 148 458 1065
Fragments arranged by size:
1065 458 148
StyI C'CwwG_G
Cuts at: 0 330 1289 1478 1671
Size: 330 959 189 193
Fragments arranged by size:
959 330 193 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 892 1491 1671
Size: 892 599 180
Fragments arranged by size:
892 599 180
TatI w'GTAC_w
Cuts at: 0 221 806 1109 1671
Size: 221 585 303 562
Fragments arranged by size:
585 562 303 221
Tth111I GACn'n_nGTC
Cuts at: 0 1504 1671
Size: 1504 167
XmaI C'CCGG_G
Cuts at: 0 1358 1671
Size: 1358 313
XmnI GAAnn'nnTTC
Cuts at: 0 542 625 1671
Size: 542 83 1046
Fragments arranged by size:
1046 542 83
Enzymes that do cut:
AarI AcuI AflII AloI AlwNI ApaLI ApoI AvaI
BanI BbeI BbsI BciVI BfrBI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI
BstEII BstXI BtgZI BtsI CspCI DrdI EaeI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PflMI PpiI PpuMI PvuII SanDI SfcI SfoI
SmaI SmlI StyI TaqII TatI Tth111I XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbvCI BcgI BclI BglI BglII BlpI
BmgBI BmtI BplI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstYI
BstZ17I Bsu36I BtgI ClaI DraI DraIII EagI EarI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HpaI
KpnI MfeI MluI NcoI NdeI NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XcmI XhoI ZraI