(Linear) (Six-base) MAPSORT of: KIBB8163.seq  Check: 7034  from: 1  to: 369



KIBB8163.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AflIII A'CryG_T

Cuts at:      0     218     369 
   Size:        218     151
 
AvaI C'yCGr_G

Cuts at:      0      83     369 
   Size:         83     286
 
BmgBI CAC'GTC

Cuts at:      0     255     369 
   Size:        255     114
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     369 
   Size:        178     191
 
BsaWI w'CCGG_w

Cuts at:      0      50     369 
   Size:         50     319
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     181     369 
   Size:        181     188
 
BsrBI CCG'CTC

Cuts at:      0     106     369 
   Size:        106     263
 
BsrDI GCAATG_nn'

Cuts at:      0     312     369 
   Size:        312      57
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     255     308     369 
   Size:        255      53      61

  Fragments arranged by size: 

                 255      61      53
 
EarI CTCTTCn'nnn_

Cuts at:      0     115     369 
   Size:        115     254
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     369 
   Size:        178     191
 
FspI TGC'GCA

Cuts at:      0     282     369 
   Size:        282      87
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48     369 
   Size:         16      32     321

  Fragments arranged by size: 

                 321      32      16
 
MfeI C'AATT_G

Cuts at:      0     302     369 
   Size:        302      67
 
MspA1I CmG'CkG

Cuts at:      0      10     369 
   Size:         10     359
 
NspI r_CATG'y

Cuts at:      0     222     369 
   Size:        222     147
 
PciI A'CATG_T

Cuts at:      0     218     369 
   Size:        218     151
 
PfoI T'CCnGG_A

Cuts at:      0     194     369 
   Size:        194     175
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     205     237     369 
   Size:        205      32     132

  Fragments arranged by size: 

                 205     132      32
 
PstI C_TGCA'G

Cuts at:      0      95     369 
   Size:         95     274
 
SfcI C'TryA_G

Cuts at:      0      91     369 
   Size:         91     278
 
SmaI CCC'GGG

Cuts at:      0      85     369 
   Size:         85     284
 
StyI C'CwwG_G

Cuts at:      0     248     347     369 
   Size:        248      99      22

  Fragments arranged by size: 

                 248      99      22
 
XmaI C'CCGG_G

Cuts at:      0      83     369 
   Size:         83     286
 
XmnI GAAnn'nnTTC

Cuts at:      0     334     369 
   Size:        334      35
 

 Enzymes that do cut:

   AflIII     AvaI    BmgBI     BpmI    BsaWI    BseRI    BsrBI    BsrDI
    BstXI     EarI  Eco57MI     FspI    Hin4I     MfeI   MspA1I     NspI
     PciI     PfoI     PpiI     PstI     SfcI     SmaI     StyI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     ZraI