Restriction map for
pF1KB8162
(Linear) (Six-base) MAPSORT of: KIBB8162.seq Check: 6590 from: 1 to: 1077
KIBB8162.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 1077
Size: 836 241
AflIII A'CryG_T
Cuts at: 0 240 1077
Size: 240 837
AleI CACnn'nnGTG
Cuts at: 0 772 1077
Size: 772 305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 436 1077
Size: 436 641
ApaLI G'TGCA_C
Cuts at: 0 118 1077
Size: 118 959
ApoI r'AATT_y
Cuts at: 0 1008 1077
Size: 1008 69
AvaI C'yCGr_G
Cuts at: 0 29 1077
Size: 29 1048
BanII G_rGCy'C
Cuts at: 0 913 1062 1077
Size: 913 149 15
Fragments arranged by size:
913 149 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 1077
Size: 134 943
BbvCI CC'TCA_GC
Cuts at: 0 143 1077
Size: 143 934
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 68 1077
Size: 34 34 1009
Fragments arranged by size:
1009 34 34
BfrBI ATG'CAT
Cuts at: 0 423 640 1077
Size: 423 217 437
Fragments arranged by size:
437 423 217
Bme1580I G_kGCm'C
Cuts at: 0 122 509 526 1077
Size: 122 387 17 551
Fragments arranged by size:
551 387 122 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 520 1077
Size: 520 557
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 72 1077
Size: 72 1005
Bpu10I CC'TnA_GC
Cuts at: 0 143 1077
Size: 143 934
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 1077
Size: 740 337
BsaI GGTCTCn'nnnn_
Cuts at: 0 464 1077
Size: 464 613
BsaAI yAC'GTr
Cuts at: 0 243 1077
Size: 243 834
BsaWI w'CCGG_w
Cuts at: 0 306 1077
Size: 306 771
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 1077
Size: 310 767
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 448 1077
Size: 148 300 629
Fragments arranged by size:
629 300 148
BsiHKAI G_wGCw'C
Cuts at: 0 122 651 1077
Size: 122 529 426
Fragments arranged by size:
529 426 122
BsiWI C'GTAC_G
Cuts at: 0 535 1077
Size: 535 542
BsmI GAATG_Cn'
Cuts at: 0 425 1077
Size: 425 652
Bsp1286I G_dGCh'C
Cuts at: 0 122 509 526 651 913 1062 1077
Size: 122 387 17 125 262 149 15
Fragments arranged by size:
387 262 149 125 122 17 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 1077
Size: 170 907
BsrBI CCG'CTC
Cuts at: 0 357 680 1077
Size: 357 323 397
Fragments arranged by size:
397 357 323
BsrFI r'CCGG_y
Cuts at: 0 443 1077
Size: 443 634
BstAPI GCAn_nnn'nTGC
Cuts at: 0 367 1077
Size: 367 710
BstBI TT'CG_AA
Cuts at: 0 591 1077
Size: 591 486
BtgI C'CryG_G
Cuts at: 0 605 1077
Size: 605 472
BtsI GCAGTG_nn'
Cuts at: 0 377 913 1077
Size: 377 536 164
Fragments arranged by size:
536 377 164
EarI CTCTTCn'nnn_
Cuts at: 0 67 1077
Size: 67 1010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 1077
Size: 836 241
EcoO109I rG'GnC_Cy
Cuts at: 0 387 1077
Size: 387 690
EcoRI G'AATT_C
Cuts at: 0 1008 1077
Size: 1008 69
HaeII r_GCGC'y
Cuts at: 0 842 1077
Size: 842 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 659 691 1077
Size: 659 32 386
Fragments arranged by size:
659 386 32
HindIII A'AGCT_T
Cuts at: 0 175 224 1077
Size: 175 49 853
Fragments arranged by size:
853 175 49
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 1077
Size: 927 150
MslI CAynn'nnrTG
Cuts at: 0 196 772 1077
Size: 196 576 305
Fragments arranged by size:
576 305 196
MspA1I CmG'CkG
Cuts at: 0 142 700 709 856 1077
Size: 142 558 9 147 221
Fragments arranged by size:
558 221 147 142 9
NaeI GCC'GGC
Cuts at: 0 445 1077
Size: 445 632
NcoI C'CATG_G
Cuts at: 0 605 1077
Size: 605 472
NdeI CA'TA_TG
Cuts at: 0 642 1077
Size: 642 435
NgoMIV G'CCGG_C
Cuts at: 0 443 1077
Size: 443 634
NsiI A_TGCA'T
Cuts at: 0 425 642 1077
Size: 425 217 435
Fragments arranged by size:
435 425 217
NspI r_CATG'y
Cuts at: 0 427 1077
Size: 427 650
PflMI CCAn_nnn'nTGG
Cuts at: 0 835 1077
Size: 835 242
PmlI CAC'GTG
Cuts at: 0 243 1077
Size: 243 834
PstI C_TGCA'G
Cuts at: 0 876 930 1077
Size: 876 54 147
Fragments arranged by size:
876 147 54
PvuII CAG'CTG
Cuts at: 0 142 856 1077
Size: 142 714 221
Fragments arranged by size:
714 221 142
SfcI C'TryA_G
Cuts at: 0 38 872 926 1077
Size: 38 834 54 151
Fragments arranged by size:
834 151 54 38
SmaI CCC'GGG
Cuts at: 0 31 1077
Size: 31 1046
SmlI C'TyrA_G
Cuts at: 0 755 1077
Size: 755 322
StyI C'CwwG_G
Cuts at: 0 605 1077
Size: 605 472
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1070 1077
Size: 1070 7
Tth111I GACn'n_nGTC
Cuts at: 0 741 1077
Size: 741 336
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 240 1077
Size: 240 837
XmaI C'CCGG_G
Cuts at: 0 29 1077
Size: 29 1048
XmnI GAAnn'nnTTC
Cuts at: 0 10 1077
Size: 10 1067
Enzymes that do cut:
AcuI AflIII AleI AlfI ApaLI ApoI AvaI BanII
BbsI BbvCI BcgI BfrBI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaWI BseRI BsgI BsiHKAI BsiWI
BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstBI BtgI
BtsI EarI Eco57MI EcoO109I EcoRI HaeII Hin4I HindIII
MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI
NspI PflMI PmlI PstI PvuII SfcI SmaI SmlI
StyI TaqII Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BciVI BclI BglI BglII
BlpI BmgBI BmtI BpmI BsaBI BsaHI BsaXI BseYI
BsiEI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MscI NarI NheI NotI NruI
PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI XbaI XhoI ZraI