(Linear) (Six-base) MAPSORT of: KIBB8159.seq  Check: 9776  from: 1  to: 420



KIBB8159.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     272     420 
   Size:        272     148
 
BclI T'GATC_A

Cuts at:      0     218     420 
   Size:        218     202
 
BsaAI yAC'GTr

Cuts at:      0      21     420 
   Size:         21     399
 
BsaHI Gr'CG_yC

Cuts at:      0      80     113     420 
   Size:         80      33     307

  Fragments arranged by size: 

                 307      80      33
 
BsaWI w'CCGG_w

Cuts at:      0     263     420 
   Size:        263     157
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     234     420 
   Size:        234     186
 
BsiEI CG_ry'CG

Cuts at:      0     107     420 
   Size:        107     313
 
BspEI T'CCGG_A

Cuts at:      0     263     420 
   Size:        263     157
 
BsrFI r'CCGG_y

Cuts at:      0       4     420 
   Size:          4     416
 
BtgI C'CryG_G

Cuts at:      0     101     357     420 
   Size:        101     256      63

  Fragments arranged by size: 

                 256     101      63
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      53     420 
   Size:         53     367
 
EaeI y'GGCC_r

Cuts at:      0       2     104     420 
   Size:          2     102     316

  Fragments arranged by size: 

                 316     102       2
 
EagI C'GGCC_G

Cuts at:      0     104     420 
   Size:        104     316
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     380     420 
   Size:        380      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     258     290     314     346     420 
   Size:        258      32      24      32      74

  Fragments arranged by size: 

                 258      74      32      32      24
 
NcoI C'CATG_G

Cuts at:      0     357     420 
   Size:        357      63
 
SmlI C'TyrA_G

Cuts at:      0     402     420 
   Size:        402      18
 
StyI C'CwwG_G

Cuts at:      0     357     420 
   Size:        357      63
 

 Enzymes that do cut:

     AhdI     BclI    BsaAI    BsaHI    BsaWI     BsgI    BsiEI    BspEI
    BsrFI     BtgI    BtgZI     EaeI     EagI     EciI    Hin4I     NcoI
     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI    BseRI    BseYI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI