Restriction map for
pF1KB8155
(Linear) (Six-base) MAPSORT of: KIBB8155.seq Check: 3933 from: 1 to: 1380
KIBB8155.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1023 1380
Size: 1023 357
AatII G_ACGT'C
Cuts at: 0 1373 1380
Size: 1373 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 928 1380
Size: 503 425 452
Fragments arranged by size:
503 452 425
AhdI GACnn_n'nnGTC
Cuts at: 0 771 1380
Size: 771 609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 815 1380
Size: 815 565
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 662 694 1380
Size: 662 32 686
Fragments arranged by size:
686 662 32
AlwNI CAG_nnn'CTG
Cuts at: 0 145 583 1380
Size: 145 438 797
Fragments arranged by size:
797 438 145
ApaI G_GGCC'C
Cuts at: 0 229 1380
Size: 229 1151
ApaLI G'TGCA_C
Cuts at: 0 1297 1380
Size: 1297 83
AvaI C'yCGr_G
Cuts at: 0 115 776 1380
Size: 115 661 604
Fragments arranged by size:
661 604 115
BanI G'GyrC_C
Cuts at: 0 64 1380
Size: 64 1316
BanII G_rGCy'C
Cuts at: 0 229 631 1294 1380
Size: 229 402 663 86
Fragments arranged by size:
663 402 229 86
BbeI G_GCGC'C
Cuts at: 0 68 1380
Size: 68 1312
BbsI GAAGACnn'nnnn_
Cuts at: 0 1305 1380
Size: 1305 75
BbvCI CC'TCA_GC
Cuts at: 0 56 1380
Size: 56 1324
BciVI GTATCCnnnnn_n'
Cuts at: 0 21 1380
Size: 21 1359
BglI GCCn_nnn'nGGC
Cuts at: 0 380 1380
Size: 380 1000
BglII A'GATC_T
Cuts at: 0 1361 1380
Size: 1361 19
BlpI GC'TnA_GC
Cuts at: 0 159 304 576 854 1288 1380
Size: 159 145 272 278 434 92
Fragments arranged by size:
434 278 272 159 145 92
Bme1580I G_kGCm'C
Cuts at: 0 229 1301 1380
Size: 229 1072 79
Fragments arranged by size:
1072 229 79
BmrI ACTGGGnnnn_n'
Cuts at: 0 186 765 1380
Size: 186 579 615
Fragments arranged by size:
615 579 186
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 174 1380
Size: 174 1206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1312 1380
Size: 1312 68
Bpu10I CC'TnA_GC
Cuts at: 0 56 1380
Size: 56 1324
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1380
Size: 650 730
BsaAI yAC'GTr
Cuts at: 0 1194 1380
Size: 1194 186
BsaHI Gr'CG_yC
Cuts at: 0 65 1370 1380
Size: 65 1305 10
Fragments arranged by size:
1305 65 10
BsaWI w'CCGG_w
Cuts at: 0 102 123 494 1380
Size: 102 21 371 886
Fragments arranged by size:
886 371 102 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 517 547 703 733 1380
Size: 265 30 222 30 156 30 647
Fragments arranged by size:
647 265 222 156 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 1380
Size: 109 1271
BseYI C'CCAG_C
Cuts at: 0 228 243 1083 1380
Size: 228 15 840 297
Fragments arranged by size:
840 297 228 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1043 1174 1380
Size: 1043 131 206
Fragments arranged by size:
1043 206 131
BsiEI CG_ry'CG
Cuts at: 0 1139 1143 1380
Size: 1139 4 237
Fragments arranged by size:
1139 237 4
BsiHKAI G_wGCw'C
Cuts at: 0 305 1294 1301 1380
Size: 305 989 7 79
Fragments arranged by size:
989 305 79 7
Bsp1286I G_dGCh'C
Cuts at: 0 229 305 631 1294 1301 1380
Size: 229 76 326 663 7 79
Fragments arranged by size:
663 326 229 79 76 7
BspEI T'CCGG_A
Cuts at: 0 102 123 494 1380
Size: 102 21 371 886
Fragments arranged by size:
886 371 102 21
BspHI T'CATG_A
Cuts at: 0 1262 1380
Size: 1262 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 397 1023 1299 1380
Size: 397 626 276 81
Fragments arranged by size:
626 397 276 81
BsrFI r'CCGG_y
Cuts at: 0 431 1142 1380
Size: 431 711 238
Fragments arranged by size:
711 431 238
BssSI C'ACGA_G
Cuts at: 0 15 250 961 1375 1380
Size: 15 235 711 414 5
Fragments arranged by size:
711 414 235 15 5
BstYI r'GATC_y
Cuts at: 0 189 491 607 1361 1380
Size: 189 302 116 754 19
Fragments arranged by size:
754 302 189 116 19
BtgI C'CryG_G
Cuts at: 0 109 348 1380
Size: 109 239 1032
Fragments arranged by size:
1032 239 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1101 1380
Size: 1101 279
BtsI GCAGTG_nn'
Cuts at: 0 345 433 475 712 1380
Size: 345 88 42 237 668
Fragments arranged by size:
668 345 237 88 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 238 273 1380
Size: 238 35 1107
Fragments arranged by size:
1107 238 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 133 1380
Size: 133 1247
EaeI y'GGCC_r
Cuts at: 0 818 1136 1380
Size: 818 318 244
Fragments arranged by size:
818 318 244
EagI C'GGCC_G
Cuts at: 0 1136 1380
Size: 1136 244
EarI CTCTTCn'nnn_
Cuts at: 0 191 1380
Size: 191 1189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 398 1380
Size: 398 982
EcoICRI GAG'CTC
Cuts at: 0 1292 1380
Size: 1292 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 928 1312 1380
Size: 503 425 384 68
Fragments arranged by size:
503 425 384 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 907 1380
Size: 907 473
EcoO109I rG'GnC_Cy
Cuts at: 0 225 1380
Size: 225 1155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 345 1029 1380
Size: 345 684 351
Fragments arranged by size:
684 351 345
FspI TGC'GCA
Cuts at: 0 439 1282 1380
Size: 439 843 98
Fragments arranged by size:
843 439 98
HaeII r_GCGC'y
Cuts at: 0 68 366 954 1204 1380
Size: 68 298 588 250 176
Fragments arranged by size:
588 298 250 176 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 517 549 757 789 1380
Size: 265 32 220 32 208 32 591
Fragments arranged by size:
591 265 220 208 32 32 32
KasI G'GCGC_C
Cuts at: 0 64 1380
Size: 64 1316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 1337 1380
Size: 752 585 43
Fragments arranged by size:
752 585 43
MscI TGG'CCA
Cuts at: 0 820 1380
Size: 820 560
MslI CAynn'nnrTG
Cuts at: 0 1261 1380
Size: 1261 119
MspA1I CmG'CkG
Cuts at: 0 308 661 1242 1380
Size: 308 353 581 138
Fragments arranged by size:
581 353 308 138
NaeI GCC'GGC
Cuts at: 0 433 1380
Size: 433 947
NarI GG'CG_CC
Cuts at: 0 65 1380
Size: 65 1315
NgoMIV G'CCGG_C
Cuts at: 0 431 1380
Size: 431 949
NspI r_CATG'y
Cuts at: 0 1260 1380
Size: 1260 120
PasI CC'CwG_GG
Cuts at: 0 756 1380
Size: 756 624
PflMI CCAn_nnn'nTGG
Cuts at: 0 687 1380
Size: 687 693
PfoI T'CCnGG_A
Cuts at: 0 833 1328 1380
Size: 833 495 52
Fragments arranged by size:
833 495 52
PspOMI G'GGCC_C
Cuts at: 0 225 1380
Size: 225 1155
PstI C_TGCA'G
Cuts at: 0 169 707 878 1034 1380
Size: 169 538 171 156 346
Fragments arranged by size:
538 346 171 169 156
PvuII CAG'CTG
Cuts at: 0 308 1242 1380
Size: 308 934 138
Fragments arranged by size:
934 308 138
SacI G_AGCT'C
Cuts at: 0 1294 1380
Size: 1294 86
SbfI CC_TGCA'GG
Cuts at: 0 1034 1380
Size: 1034 346
SfcI C'TryA_G
Cuts at: 0 165 703 874 999 1030 1380
Size: 165 538 171 125 31 350
Fragments arranged by size:
538 350 171 165 125 31
SfoI GGC'GCC
Cuts at: 0 66 1380
Size: 66 1314
SmaI CCC'GGG
Cuts at: 0 117 1380
Size: 117 1263
SmlI C'TyrA_G
Cuts at: 0 665 1380
Size: 665 715
SphI G_CATG'C
Cuts at: 0 1260 1380
Size: 1260 120
StuI AGG'CCT
Cuts at: 0 280 1028 1380
Size: 280 748 352
Fragments arranged by size:
748 352 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 96 1380
Size: 96 1284
Tth111I GACn'n_nGTC
Cuts at: 0 178 1380
Size: 178 1202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 21 1080 1380
Size: 21 1059 300
Fragments arranged by size:
1059 300 21
XmaI C'CCGG_G
Cuts at: 0 115 1380
Size: 115 1265
XmnI GAAnn'nnTTC
Cuts at: 0 991 1380
Size: 991 389
ZraI GAC'GTC
Cuts at: 0 1371 1380
Size: 1371 9
Enzymes that do cut:
AarI AatII AcuI AhdI AlfI AloI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BciVI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrFI BssSI
BstYI BtgI BtgZI BtsI CspCI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PflMI PfoI PspOMI PstI
PvuII SacI SbfI SfcI SfoI SmaI SmlI SphI
StuI TaqII Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AleI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BclI BfrBI BmgBI BmtI BsaI BsaBI BsiWI BsmI
BsmBI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I ClaI DraI DraIII EcoRI EcoRV
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StyI
SwaI TatI TstI XbaI XhoI