Restriction map for
pF1KB8153
(Linear) (Six-base) MAPSORT of: KIBB8153.seq Check: 7658 from: 1 to: 1239
KIBB8153.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 1239
Size: 707 532
AhdI GACnn_n'nnGTC
Cuts at: 0 102 1239
Size: 102 1137
AleI CACnn'nnGTG
Cuts at: 0 781 1239
Size: 781 458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1211 1239
Size: 1211 28
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 695 727 1239
Size: 695 32 512
Fragments arranged by size:
695 512 32
AlwNI CAG_nnn'CTG
Cuts at: 0 93 156 1239
Size: 93 63 1083
Fragments arranged by size:
1083 93 63
ApoI r'AATT_y
Cuts at: 0 643 1239
Size: 643 596
AseI AT'TA_AT
Cuts at: 0 1076 1239
Size: 1076 163
AvaI C'yCGr_G
Cuts at: 0 234 325 561 1124 1239
Size: 234 91 236 563 115
Fragments arranged by size:
563 236 234 115 91
BanI G'GyrC_C
Cuts at: 0 37 143 297 342 799 1120 1239
Size: 37 106 154 45 457 321 119
Fragments arranged by size:
457 321 154 119 106 45 37
BanII G_rGCy'C
Cuts at: 0 223 1239
Size: 223 1016
BbeI G_GCGC'C
Cuts at: 0 41 147 301 346 1239
Size: 41 106 154 45 893
Fragments arranged by size:
893 154 106 45 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 695 1239
Size: 695 544
BglI GCCn_nnn'nGGC
Cuts at: 0 329 1239
Size: 329 910
BlpI GC'TnA_GC
Cuts at: 0 308 428 1239
Size: 308 120 811
Fragments arranged by size:
811 308 120
Bme1580I G_kGCm'C
Cuts at: 0 802 1239
Size: 802 437
BmgBI CAC'GTC
Cuts at: 0 553 1239
Size: 553 686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 1090 1239
Size: 803 287 149
Fragments arranged by size:
803 287 149
BsaHI Gr'CG_yC
Cuts at: 0 38 144 298 343 1239
Size: 38 106 154 45 896
Fragments arranged by size:
896 154 106 45 38
BsaWI w'CCGG_w
Cuts at: 0 932 1037 1239
Size: 932 105 202
Fragments arranged by size:
932 202 105
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 802 826 1130 1239
Size: 802 24 304 109
Fragments arranged by size:
802 304 109 24
BseYI C'CCAG_C
Cuts at: 0 222 1064 1239
Size: 222 842 175
Fragments arranged by size:
842 222 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1239
Size: 743 496
BsiEI CG_ry'CG
Cuts at: 0 194 261 1239
Size: 194 67 978
Fragments arranged by size:
978 194 67
Bsp1286I G_dGCh'C
Cuts at: 0 223 802 1239
Size: 223 579 437
Fragments arranged by size:
579 437 223
BspEI T'CCGG_A
Cuts at: 0 932 1037 1239
Size: 932 105 202
Fragments arranged by size:
932 202 105
BsrBI CCG'CTC
Cuts at: 0 432 1239
Size: 432 807
BsrFI r'CCGG_y
Cuts at: 0 140 199 247 1239
Size: 140 59 48 992
Fragments arranged by size:
992 140 59 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 156 1239
Size: 156 1083
BstBI TT'CG_AA
Cuts at: 0 714 1239
Size: 714 525
BstEII G'GTnAC_C
Cuts at: 0 1084 1239
Size: 1084 155
BstXI CCAn_nnnn'nTGG
Cuts at: 0 747 783 966 1239
Size: 747 36 183 273
Fragments arranged by size:
747 273 183 36
BstYI r'GATC_y
Cuts at: 0 436 1239
Size: 436 803
BtgI C'CryG_G
Cuts at: 0 23 207 399 965 1239
Size: 23 184 192 566 274
Fragments arranged by size:
566 274 192 184 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 58 1239
Size: 58 1181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 681 716 1239
Size: 681 35 523
Fragments arranged by size:
681 523 35
EaeI y'GGCC_r
Cuts at: 0 71 191 258 1239
Size: 71 120 67 981
Fragments arranged by size:
981 120 71 67
EagI C'GGCC_G
Cuts at: 0 191 258 1239
Size: 191 67 981
Fragments arranged by size:
981 191 67
EarI CTCTTCn'nnn_
Cuts at: 0 363 803 1239
Size: 363 440 436
Fragments arranged by size:
440 436 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 803 1090 1239
Size: 707 96 287 149
Fragments arranged by size:
707 287 149 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 127 368 1239
Size: 127 241 871
Fragments arranged by size:
871 241 127
EcoO109I rG'GnC_Cy
Cuts at: 0 230 1239
Size: 230 1009
HaeII r_GCGC'y
Cuts at: 0 11 41 141 147 301 346 1239
Size: 11 30 100 6 154 45 893
Fragments arranged by size:
893 154 100 45 30 11 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 421 453 1045 1077 1239
Size: 88 32 301 32 592 32 162
Fragments arranged by size:
592 301 162 88 32 32 32
KasI G'GCGC_C
Cuts at: 0 37 143 297 342 1239
Size: 37 106 154 45 897
Fragments arranged by size:
897 154 106 45 37
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 818 1239
Size: 86 732 421
Fragments arranged by size:
732 421 86
MscI TGG'CCA
Cuts at: 0 73 1239
Size: 73 1166
MslI CAynn'nnrTG
Cuts at: 0 781 1239
Size: 781 458
MspA1I CmG'CkG
Cuts at: 0 34 90 156 209 312 490 1068 1239
Size: 34 56 66 53 103 178 578 171
Fragments arranged by size:
578 178 171 103 66 56 53 34
NaeI GCC'GGC
Cuts at: 0 142 249 1239
Size: 142 107 990
Fragments arranged by size:
990 142 107
NarI GG'CG_CC
Cuts at: 0 38 144 298 343 1239
Size: 38 106 154 45 896
Fragments arranged by size:
896 154 106 45 38
NgoMIV G'CCGG_C
Cuts at: 0 140 247 1239
Size: 140 107 992
Fragments arranged by size:
992 140 107
PasI CC'CwG_GG
Cuts at: 0 598 795 1239
Size: 598 197 444
Fragments arranged by size:
598 444 197
PstI C_TGCA'G
Cuts at: 0 161 341 1239
Size: 161 180 898
Fragments arranged by size:
898 180 161
PvuII CAG'CTG
Cuts at: 0 90 156 490 1068 1239
Size: 90 66 334 578 171
Fragments arranged by size:
578 334 171 90 66
SacII CC_GC'GG
Cuts at: 0 210 1239
Size: 210 1029
SbfI CC_TGCA'GG
Cuts at: 0 341 1239
Size: 341 898
SexAI A'CCwGG_T
Cuts at: 0 513 1239
Size: 513 726
SfcI C'TryA_G
Cuts at: 0 157 337 441 1239
Size: 157 180 104 798
Fragments arranged by size:
798 180 157 104
SfoI GGC'GCC
Cuts at: 0 39 145 299 344 1239
Size: 39 106 154 45 895
Fragments arranged by size:
895 154 106 45 39
SgrAI Cr'CCGG_yG
Cuts at: 0 140 1239
Size: 140 1099
SmaI CCC'GGG
Cuts at: 0 327 1126 1239
Size: 327 799 113
Fragments arranged by size:
799 327 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 688 1239
Size: 688 551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1176 1239
Size: 1176 63
XmaI C'CCGG_G
Cuts at: 0 325 1124 1239
Size: 325 799 115
Fragments arranged by size:
799 325 115
Enzymes that do cut:
AcuI AhdI AleI AlfI AloI AlwNI ApoI AseI
AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I
BmgBI BpmI BsaHI BsaWI BseRI BseYI BsgI BsiEI
Bsp1286I BspEI BsrBI BsrFI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI CspCI EaeI EagI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV PasI PstI PvuII SacII
SbfI SexAI SfcI SfoI SgrAI SmaI TaqII XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsiHKAI
BsiWI BsmI BsmBI BspHI BspMI BsrDI BsrGI BssHII
BssSI BstZ17I Bsu36I BtsI ClaI DraI DraIII DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
ScaI SfiI SgfI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TatI TstI Tth111I XbaI
XhoI XmnI ZraI