Restriction map for
pF1KB8150
(Linear) (Six-base) MAPSORT of: KIBB8150.seq Check: 7883 from: 1 to: 1437
KIBB8150.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AccI GT'mk_AC
Cuts at: 0 1104 1437
Size: 1104 333
AflIII A'CryG_T
Cuts at: 0 570 1437
Size: 570 867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 225 1437
Size: 225 1212
AlwNI CAG_nnn'CTG
Cuts at: 0 277 670 1437
Size: 277 393 767
Fragments arranged by size:
767 393 277
ApaI G_GGCC'C
Cuts at: 0 643 1437
Size: 643 794
ApoI r'AATT_y
Cuts at: 0 683 706 1044 1437
Size: 683 23 338 393
Fragments arranged by size:
683 393 338 23
AseI AT'TA_AT
Cuts at: 0 1202 1437
Size: 1202 235
AvaI C'yCGr_G
Cuts at: 0 194 1437
Size: 194 1243
BamHI G'GATC_C
Cuts at: 0 190 1437
Size: 190 1247
BanII G_rGCy'C
Cuts at: 0 643 761 1437
Size: 643 118 676
Fragments arranged by size:
676 643 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 158 254 1437
Size: 158 96 1183
Fragments arranged by size:
1183 158 96
Bme1580I G_kGCm'C
Cuts at: 0 643 1437
Size: 643 794
BmrI ACTGGGnnnn_n'
Cuts at: 0 1330 1437
Size: 1330 107
BmtI G_CTAG'C
Cuts at: 0 1261 1437
Size: 1261 176
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 1252 1437
Size: 647 605 185
Fragments arranged by size:
647 605 185
Bpu10I CC'TnA_GC
Cuts at: 0 76 422 1437
Size: 76 346 1015
Fragments arranged by size:
1015 346 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 805 1437
Size: 304 501 632
Fragments arranged by size:
632 501 304
BsaI GGTCTCn'nnnn_
Cuts at: 0 906 1437
Size: 906 531
BsaAI yAC'GTr
Cuts at: 0 457 1437
Size: 457 980
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 542 572 1437
Size: 542 30 865
Fragments arranged by size:
865 542 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 769 1249 1437
Size: 769 480 188
Fragments arranged by size:
769 480 188
BseYI C'CCAG_C
Cuts at: 0 52 1437
Size: 52 1385
BsiHKAI G_wGCw'C
Cuts at: 0 603 761 1437
Size: 603 158 676
Fragments arranged by size:
676 603 158
BsmI GAATG_Cn'
Cuts at: 0 998 1437
Size: 998 439
Bsp1286I G_dGCh'C
Cuts at: 0 603 643 761 1437
Size: 603 40 118 676
Fragments arranged by size:
676 603 118 40
BssSI C'ACGA_G
Cuts at: 0 596 1437
Size: 596 841
BstBI TT'CG_AA
Cuts at: 0 1315 1437
Size: 1315 122
BstYI r'GATC_y
Cuts at: 0 190 274 731 1437
Size: 190 84 457 706
Fragments arranged by size:
706 457 190 84
Bsu36I CC'TnA_GG
Cuts at: 0 671 1437
Size: 671 766
BtsI GCAGTG_nn'
Cuts at: 0 407 1437
Size: 407 1030
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 383 418 1437
Size: 383 35 1019
Fragments arranged by size:
1019 383 35
EarI CTCTTCn'nnn_
Cuts at: 0 692 956 1437
Size: 692 264 481
Fragments arranged by size:
692 481 264
EcoICRI GAG'CTC
Cuts at: 0 759 1437
Size: 759 678
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 1252 1437
Size: 647 605 185
Fragments arranged by size:
647 605 185
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1058 1437
Size: 1058 379
EcoO109I rG'GnC_Cy
Cuts at: 0 639 1133 1437
Size: 639 494 304
Fragments arranged by size:
639 494 304
FspI TGC'GCA
Cuts at: 0 1018 1437
Size: 1018 419
FspAI rTGC'GCAy
Cuts at: 0 1018 1437
Size: 1018 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1120 1152 1188 1220 1437
Size: 1120 32 36 32 217
Fragments arranged by size:
1120 217 36 32 32
MfeI C'AATT_G
Cuts at: 0 816 1437
Size: 816 621
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1437
Size: 914 523
MspA1I CmG'CkG
Cuts at: 0 124 262 1297 1437
Size: 124 138 1035 140
Fragments arranged by size:
1035 140 138 124
NheI G'CTAG_C
Cuts at: 0 1257 1437
Size: 1257 180
NspI r_CATG'y
Cuts at: 0 87 351 1437
Size: 87 264 1086
Fragments arranged by size:
1086 264 87
PmlI CAC'GTG
Cuts at: 0 457 1437
Size: 457 980
PspOMI G'GGCC_C
Cuts at: 0 639 1437
Size: 639 798
PstI C_TGCA'G
Cuts at: 0 402 1032 1437
Size: 402 630 405
Fragments arranged by size:
630 405 402
PvuII CAG'CTG
Cuts at: 0 124 262 1297 1437
Size: 124 138 1035 140
Fragments arranged by size:
1035 140 138 124
SacI G_AGCT'C
Cuts at: 0 761 1437
Size: 761 676
SfcI C'TryA_G
Cuts at: 0 398 1028 1437
Size: 398 630 409
Fragments arranged by size:
630 409 398
SmlI C'TyrA_G
Cuts at: 0 283 784 1437
Size: 283 501 653
Fragments arranged by size:
653 501 283
SpeI A'CTAG_T
Cuts at: 0 873 1246 1437
Size: 873 373 191
Fragments arranged by size:
873 373 191
SphI G_CATG'C
Cuts at: 0 87 1437
Size: 87 1350
StyI C'CwwG_G
Cuts at: 0 520 896 1437
Size: 520 376 541
Fragments arranged by size:
541 520 376
TatI w'GTAC_w
Cuts at: 0 870 1437
Size: 870 567
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 83 115 182 214 542 574 796 828
Size: 83 32 67 32 328 32 222 32
Cuts at: 828 1160 1192 1322 1354 1437
Size: 332 32 130 32 83
Fragments arranged by size:
332 328 222 130 83 83 67 32
32 32 32 32 32
Enzymes that do cut:
AccI AflIII AlfI AlwNI ApaI ApoI AseI AvaI
BamHI BanII BbsI Bme1580I BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaXI BseRI BseYI BsiHKAI BsmI
Bsp1286I BssSI BstBI BstYI Bsu36I BtsI CspCI EarI
EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI Hin4I MfeI
MmeI MspA1I NheI NspI PmlI PspOMI PstI PvuII
SacI SfcI SmlI SpeI SphI StyI TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AsiSI AvrII BaeI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BplI BsaBI BsaHI BsaWI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstEII BstXI BstZ17I BtgI
BtgZI ClaI DraI DraIII DrdI EaeI EagI EciI
EcoRI EcoRV FalI FseI HaeII HincII HindIII HpaI
KasI KpnI MluI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI