Restriction map for
pF1KB8148
(Linear) (Six-base) MAPSORT of: KIBB8148.seq Check: 6708 from: 1 to: 1629
KIBB8148.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1436 1496 1526 1589 1629
Size: 688 748 60 30 63 40
Fragments arranged by size:
748 688 63 60 40 30
AfeI AGC'GCT
Cuts at: 0 983 1035 1629
Size: 983 52 594
Fragments arranged by size:
983 594 52
AhdI GACnn_n'nnGTC
Cuts at: 0 249 912 1629
Size: 249 663 717
Fragments arranged by size:
717 663 249
AleI CACnn'nnGTG
Cuts at: 0 1210 1629
Size: 1210 419
AlwNI CAG_nnn'CTG
Cuts at: 0 1005 1629
Size: 1005 624
ApaLI G'TGCA_C
Cuts at: 0 70 322 676 1629
Size: 70 252 354 953
Fragments arranged by size:
953 354 252 70
AvaI C'yCGr_G
Cuts at: 0 65 625 1629
Size: 65 560 1004
Fragments arranged by size:
1004 560 65
BanI G'GyrC_C
Cuts at: 0 132 592 1629
Size: 132 460 1037
Fragments arranged by size:
1037 460 132
BanII G_rGCy'C
Cuts at: 0 626 1246 1409 1629
Size: 626 620 163 220
Fragments arranged by size:
626 620 220 163
BbeI G_GCGC'C
Cuts at: 0 136 596 1629
Size: 136 460 1033
Fragments arranged by size:
1033 460 136
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 1629
Size: 134 1495
BbvCI CC'TCA_GC
Cuts at: 0 827 1388 1629
Size: 827 561 241
Fragments arranged by size:
827 561 241
BglI GCCn_nnn'nGGC
Cuts at: 0 601 1629
Size: 601 1028
BglII A'GATC_T
Cuts at: 0 725 1629
Size: 725 904
BlpI GC'TnA_GC
Cuts at: 0 111 547 1629
Size: 111 436 1082
Fragments arranged by size:
1082 436 111
Bme1580I G_kGCm'C
Cuts at: 0 74 326 680 1629
Size: 74 252 354 949
Fragments arranged by size:
949 354 252 74
BmgBI CAC'GTC
Cuts at: 0 744 1629
Size: 744 885
BmrI ACTGGGnnnn_n'
Cuts at: 0 191 1629
Size: 191 1438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1428 1629
Size: 1428 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 105 159 223 265 352 463 520
Size: 63 42 54 64 42 87 111 57
Cuts at: 520 1006 1027 1629
Size: 486 21 602
Fragments arranged by size:
602 486 111 87 64 63 57 54
42 42 21
Bpu10I CC'TnA_GC
Cuts at: 0 217 827 1388 1629
Size: 217 610 561 241
Fragments arranged by size:
610 561 241 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 757 919 1629
Size: 232 525 162 710
Fragments arranged by size:
710 525 232 162
BsaI GGTCTCn'nnnn_
Cuts at: 0 499 1183 1629
Size: 499 684 446
Fragments arranged by size:
684 499 446
BsaAI yAC'GTr
Cuts at: 0 54 1629
Size: 54 1575
BsaHI Gr'CG_yC
Cuts at: 0 61 133 560 593 887 1629
Size: 61 72 427 33 294 742
Fragments arranged by size:
742 427 294 72 61 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 1629
Size: 19 30 1580
Fragments arranged by size:
1580 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 162 517 553 700 1114 1201 1360 1444
Size: 162 355 36 147 414 87 159 84
Cuts at: 1444 1447 1450 1480 1588 1591 1629
Size: 3 3 30 108 3 38
Fragments arranged by size:
414 355 162 159 147 108 87 84
38 36 30 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1597 1629
Size: 1597 32
BsiHKAI G_wGCw'C
Cuts at: 0 74 326 626 680 1246 1629
Size: 74 252 300 54 566 383
Fragments arranged by size:
566 383 300 252 74 54
BsmBI CGTCTCn'nnnn_
Cuts at: 0 52 1629
Size: 52 1577
Bsp1286I G_dGCh'C
Cuts at: 0 74 326 626 680 1246 1409 1629
Size: 74 252 300 54 566 163 220
Fragments arranged by size:
566 300 252 220 163 74 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 443 527 1629
Size: 443 84 1102
Fragments arranged by size:
1102 443 84
BsrBI CCG'CTC
Cuts at: 0 1310 1629
Size: 1310 319
BsrFI r'CCGG_y
Cuts at: 0 135 569 1629
Size: 135 434 1060
Fragments arranged by size:
1060 434 135
BssHII G'CGCG_C
Cuts at: 0 526 583 785 894 1629
Size: 526 57 202 109 735
Fragments arranged by size:
735 526 202 109 57
BssSI C'ACGA_G
Cuts at: 0 325 1629
Size: 325 1304
BstXI CCAn_nnnn'nTGG
Cuts at: 0 948 1255 1629
Size: 948 307 374
Fragments arranged by size:
948 374 307
BstYI r'GATC_y
Cuts at: 0 425 710 725 1629
Size: 425 285 15 904
Fragments arranged by size:
904 425 285 15
BtgI C'CryG_G
Cuts at: 0 731 1629
Size: 731 898
EaeI y'GGCC_r
Cuts at: 0 803 1257 1629
Size: 803 454 372
Fragments arranged by size:
803 454 372
EarI CTCTTCn'nnn_
Cuts at: 0 530 677 1445 1457 1472 1493 1565 1629
Size: 530 147 768 12 15 21 72 64
Fragments arranged by size:
768 530 147 72 64 21 15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 463 753 1629
Size: 463 290 876
Fragments arranged by size:
876 463 290
EcoICRI GAG'CTC
Cuts at: 0 624 1244 1629
Size: 624 620 385
Fragments arranged by size:
624 620 385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 105 159 223 265 352 463 520
Size: 63 42 54 64 42 87 111 57
Cuts at: 520 688 1006 1027 1436 1496 1526 1589 1629
Size: 168 318 21 409 60 30 63 40
Fragments arranged by size:
409 318 168 111 87 64 63 63
60 57 54 42 42 40 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 1629
Size: 220 1409
EcoO109I rG'GnC_Cy
Cuts at: 0 285 348 1629
Size: 285 63 1281
Fragments arranged by size:
1281 285 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 864 1629
Size: 864 765
FspI TGC'GCA
Cuts at: 0 88 514 1629
Size: 88 426 1115
Fragments arranged by size:
1115 426 88
HaeII r_GCGC'y
Cuts at: 0 51 136 412 478 596 610 872 985
Size: 51 85 276 66 118 14 262 113
Cuts at: 985 1037 1629
Size: 52 592
Fragments arranged by size:
592 276 262 118 113 85 66 52
51 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 183 215 235 267 643 675 898 912
Size: 183 32 20 32 376 32 223 14
Cuts at: 912 930 944 1629
Size: 18 14 685
Fragments arranged by size:
685 376 223 183 32 32 32 20
18 14 14
KasI G'GCGC_C
Cuts at: 0 132 592 1629
Size: 132 460 1037
Fragments arranged by size:
1037 460 132
MslI CAynn'nnrTG
Cuts at: 0 1210 1629
Size: 1210 419
MspA1I CmG'CkG
Cuts at: 0 92 996 1474 1629
Size: 92 904 478 155
Fragments arranged by size:
904 478 155 92
NaeI GCC'GGC
Cuts at: 0 571 1629
Size: 571 1058
NarI GG'CG_CC
Cuts at: 0 133 593 1629
Size: 133 460 1036
Fragments arranged by size:
1036 460 133
NgoMIV G'CCGG_C
Cuts at: 0 569 1629
Size: 569 1060
NspI r_CATG'y
Cuts at: 0 819 965 1629
Size: 819 146 664
Fragments arranged by size:
819 664 146
PfoI T'CCnGG_A
Cuts at: 0 281 350 1629
Size: 281 69 1279
Fragments arranged by size:
1279 281 69
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 820 852 1629
Size: 820 32 777
Fragments arranged by size:
820 777 32
PpuMI rG'GwC_Cy
Cuts at: 0 285 348 1629
Size: 285 63 1281
Fragments arranged by size:
1281 285 63
PstI C_TGCA'G
Cuts at: 0 524 704 1001 1629
Size: 524 180 297 628
Fragments arranged by size:
628 524 297 180
PvuII CAG'CTG
Cuts at: 0 996 1474 1629
Size: 996 478 155
Fragments arranged by size:
996 478 155
SacI G_AGCT'C
Cuts at: 0 626 1246 1629
Size: 626 620 383
Fragments arranged by size:
626 620 383
SbfI CC_TGCA'GG
Cuts at: 0 524 1629
Size: 524 1105
SfcI C'TryA_G
Cuts at: 0 96 520 700 997 1629
Size: 96 424 180 297 632
Fragments arranged by size:
632 424 297 180 96
SfoI GGC'GCC
Cuts at: 0 134 594 1629
Size: 134 460 1035
Fragments arranged by size:
1035 460 134
SgrAI Cr'CCGG_yG
Cuts at: 0 135 1629
Size: 135 1494
SmaI CCC'GGG
Cuts at: 0 67 1629
Size: 67 1562
SmlI C'TyrA_G
Cuts at: 0 247 625 772 934 1629
Size: 247 378 147 162 695
Fragments arranged by size:
695 378 247 162 147
SphI G_CATG'C
Cuts at: 0 965 1629
Size: 965 664
StyI C'CwwG_G
Cuts at: 0 902 1382 1397 1493 1629
Size: 902 480 15 96 136
Fragments arranged by size:
902 480 136 96 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 877 1629
Size: 877 752
XhoI C'TCGA_G
Cuts at: 0 625 1629
Size: 625 1004
XmaI C'CCGG_G
Cuts at: 0 65 1629
Size: 65 1564
Enzymes that do cut:
AcuI AfeI AhdI AleI AlwNI ApaLI AvaI BanI
BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstXI BstYI BtgI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I KasI MslI MspA1I NaeI NarI NgoMIV
NspI PfoI PpiI PpuMI PstI PvuII SacI SbfI
SfcI SfoI SgrAI SmaI SmlI SphI StyI TaqII
XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BmtI BsaBI
BsaWI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI ScaI SexAI SfiI SgfI SnaBI SpeI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XmnI ZraI