Restriction map for
pF1KB8147
(Linear) (Six-base) MAPSORT of: KIBB8147.seq Check: 1803 from: 1 to: 804
KIBB8147.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AccI GT'mk_AC
Cuts at: 0 14 48 728 804
Size: 14 34 680 76
Fragments arranged by size:
680 76 34 14
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 136 168 804
Size: 136 32 636
Fragments arranged by size:
636 136 32
AlwNI CAG_nnn'CTG
Cuts at: 0 124 804
Size: 124 680
ApaI G_GGCC'C
Cuts at: 0 781 804
Size: 781 23
ApoI r'AATT_y
Cuts at: 0 184 316 804
Size: 184 132 488
Fragments arranged by size:
488 184 132
BanII G_rGCy'C
Cuts at: 0 781 804
Size: 781 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 629 804
Size: 629 175
BlpI GC'TnA_GC
Cuts at: 0 194 804
Size: 194 610
Bme1580I G_kGCm'C
Cuts at: 0 781 804
Size: 781 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 804
Size: 413 391
Bpu10I CC'TnA_GC
Cuts at: 0 117 804
Size: 117 687
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 802 804
Size: 802 2
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 804
Size: 491 313
BsiHKAI G_wGCw'C
Cuts at: 0 118 804
Size: 118 686
BsmBI CGTCTCn'nnnn_
Cuts at: 0 560 804
Size: 560 244
Bsp1286I G_dGCh'C
Cuts at: 0 118 781 804
Size: 118 663 23
Fragments arranged by size:
663 118 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 229 585 804
Size: 229 356 219
Fragments arranged by size:
356 229 219
BsrDI GCAATG_nn'
Cuts at: 0 164 804
Size: 164 640
BssSI C'ACGA_G
Cuts at: 0 205 804
Size: 205 599
BstZ17I GTA'TAC
Cuts at: 0 15 804
Size: 15 789
Bsu36I CC'TnA_GG
Cuts at: 0 518 804
Size: 518 286
BtsI GCAGTG_nn'
Cuts at: 0 11 413 474 804
Size: 11 402 61 330
Fragments arranged by size:
402 330 61 11
EarI CTCTTCn'nnn_
Cuts at: 0 791 804
Size: 791 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 804
Size: 413 391
EcoNI CCTnn'n_nnAGG
Cuts at: 0 770 804
Size: 770 34
EcoO109I rG'GnC_Cy
Cuts at: 0 777 804
Size: 777 27
EcoRI G'AATT_C
Cuts at: 0 316 804
Size: 316 488
HincII GTy'rAC
Cuts at: 0 49 261 600 804
Size: 49 212 339 204
Fragments arranged by size:
339 212 204 49
MspA1I CmG'CkG
Cuts at: 0 508 580 804
Size: 508 72 224
Fragments arranged by size:
508 224 72
NspI r_CATG'y
Cuts at: 0 60 150 804
Size: 60 90 654
Fragments arranged by size:
654 90 60
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 804
Size: 1 32 771
Fragments arranged by size:
771 32 1
PspOMI G'GGCC_C
Cuts at: 0 777 804
Size: 777 27
PstI C_TGCA'G
Cuts at: 0 86 408 804
Size: 86 322 396
Fragments arranged by size:
396 322 86
PvuII CAG'CTG
Cuts at: 0 580 804
Size: 580 224
SalI G'TCGA_C
Cuts at: 0 47 804
Size: 47 757
SfcI C'TryA_G
Cuts at: 0 82 404 804
Size: 82 322 400
Fragments arranged by size:
400 322 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 244 804
Size: 244 560
TatI w'GTAC_w
Cuts at: 0 209 804
Size: 209 595
Tth111I GACn'n_nGTC
Cuts at: 0 392 804
Size: 392 412
XmnI GAAnn'nnTTC
Cuts at: 0 320 340 804
Size: 320 20 464
Fragments arranged by size:
464 320 20
Enzymes that do cut:
AccI AloI AlwNI ApaI ApoI BanII BbsI BlpI
Bme1580I BpmI Bpu10I BseRI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrDI BssSI BstZ17I Bsu36I BtsI EarI Eco57MI
EcoNI EcoO109I EcoRI HincII MspA1I NspI PpiI PspOMI
PstI PvuII SalI SfcI TaqII TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TstI XbaI
XcmI XhoI XmaI ZraI