Restriction map for
pF1KB8146
(Linear) (Six-base) MAPSORT of: KIBB8146.seq Check: 9373 from: 1 to: 1119
KIBB8146.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 457 703 1119
Size: 385 72 246 416
Fragments arranged by size:
416 385 246 72
AfeI AGC'GCT
Cuts at: 0 480 1119
Size: 480 639
AhdI GACnn_n'nnGTC
Cuts at: 0 1059 1119
Size: 1059 60
AlwNI CAG_nnn'CTG
Cuts at: 0 70 669 1027 1119
Size: 70 599 358 92
Fragments arranged by size:
599 358 92 70
AvaI C'yCGr_G
Cuts at: 0 122 1119
Size: 122 997
BamHI G'GATC_C
Cuts at: 0 156 1119
Size: 156 963
BanI G'GyrC_C
Cuts at: 0 146 1119
Size: 146 973
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 307 341 1119
Size: 307 34 778
Fragments arranged by size:
778 307 34
BfrBI ATG'CAT
Cuts at: 0 571 1119
Size: 571 548
BlpI GC'TnA_GC
Cuts at: 0 698 1119
Size: 698 421
Bme1580I G_kGCm'C
Cuts at: 0 149 1119
Size: 149 970
BmrI ACTGGGnnnn_n'
Cuts at: 0 387 933 1119
Size: 387 546 186
Fragments arranged by size:
546 387 186
BmtI G_CTAG'C
Cuts at: 0 196 1119
Size: 196 923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1033 1119
Size: 1033 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1119
Size: 208 911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1119
Size: 163 956
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 365 395 1119
Size: 365 30 724
Fragments arranged by size:
724 365 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 84 733 1119
Size: 84 649 386
Fragments arranged by size:
649 386 84
BseYI C'CCAG_C
Cuts at: 0 63 764 1119
Size: 63 701 355
Fragments arranged by size:
701 355 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 1119
Size: 489 630
BsmI GAATG_Cn'
Cuts at: 0 573 1119
Size: 573 546
Bsp1286I G_dGCh'C
Cuts at: 0 149 1119
Size: 149 970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 93 1119
Size: 93 1026
BsrDI GCAATG_nn'
Cuts at: 0 357 1119
Size: 357 762
BstEII G'GTnAC_C
Cuts at: 0 846 1119
Size: 846 273
BstXI CCAn_nnnn'nTGG
Cuts at: 0 377 639 1119
Size: 377 262 480
Fragments arranged by size:
480 377 262
BstYI r'GATC_y
Cuts at: 0 156 909 1119
Size: 156 753 210
Fragments arranged by size:
753 210 156
BtgI C'CryG_G
Cuts at: 0 150 769 842 1119
Size: 150 619 73 277
Fragments arranged by size:
619 277 150 73
BtsI GCAGTG_nn'
Cuts at: 0 52 1119
Size: 52 1067
DraIII CAC_nnn'GTG
Cuts at: 0 992 1119
Size: 992 127
DrdI GACnn_nn'nnGTC
Cuts at: 0 686 1119
Size: 686 433
EaeI y'GGCC_r
Cuts at: 0 863 1119
Size: 863 256
EarI CTCTTCn'nnn_
Cuts at: 0 476 679 710 1119
Size: 476 203 31 409
Fragments arranged by size:
476 409 203 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 99 429 994 1119
Size: 99 330 565 125
Fragments arranged by size:
565 330 125 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 385 457 703 1119
Size: 208 177 72 246 416
Fragments arranged by size:
416 246 208 177 72
EcoO109I rG'GnC_Cy
Cuts at: 0 1041 1119
Size: 1041 78
EcoRV GAT'ATC
Cuts at: 0 660 1119
Size: 660 459
HaeII r_GCGC'y
Cuts at: 0 136 482 1119
Size: 136 346 637
Fragments arranged by size:
637 346 136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 200 232 644 676 1045 1077 1119
Size: 200 32 412 32 369 32 42
Fragments arranged by size:
412 369 200 42 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 599 1119
Size: 288 311 520
Fragments arranged by size:
520 311 288
MscI TGG'CCA
Cuts at: 0 865 1119
Size: 865 254
MslI CAynn'nnrTG
Cuts at: 0 262 637 1119
Size: 262 375 482
Fragments arranged by size:
482 375 262
MspA1I CmG'CkG
Cuts at: 0 581 1047 1119
Size: 581 466 72
Fragments arranged by size:
581 466 72
NcoI C'CATG_G
Cuts at: 0 842 1119
Size: 842 277
NheI G'CTAG_C
Cuts at: 0 192 1119
Size: 192 927
NsiI A_TGCA'T
Cuts at: 0 573 1119
Size: 573 546
NspI r_CATG'y
Cuts at: 0 706 762 1119
Size: 706 56 357
Fragments arranged by size:
706 357 56
PasI CC'CwG_GG
Cuts at: 0 489 618 1119
Size: 489 129 501
Fragments arranged by size:
501 489 129
PflMI CCAn_nnn'nTGG
Cuts at: 0 70 1119
Size: 70 1049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 1119
Size: 412 32 675
Fragments arranged by size:
675 412 32
PpuMI rG'GwC_Cy
Cuts at: 0 1041 1119
Size: 1041 78
PstI C_TGCA'G
Cuts at: 0 13 545 1052 1119
Size: 13 532 507 67
Fragments arranged by size:
532 507 67 13
RsrII CG'GwC_CG
Cuts at: 0 416 1119
Size: 416 703
SfcI C'TryA_G
Cuts at: 0 9 513 541 1048 1119
Size: 9 504 28 507 71
Fragments arranged by size:
507 504 71 28 9
SmaI CCC'GGG
Cuts at: 0 124 1119
Size: 124 995
SmlI C'TyrA_G
Cuts at: 0 178 1119
Size: 178 941
StyI C'CwwG_G
Cuts at: 0 164 270 842 1067 1119
Size: 164 106 572 225 52
Fragments arranged by size:
572 225 164 106 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 369 433 481 1119
Size: 369 64 48 638
Fragments arranged by size:
638 369 64 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 306 338 365 397 1119
Size: 306 32 27 32 722
Fragments arranged by size:
722 306 32 32 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 582 1119
Size: 582 537
XmaI C'CCGG_G
Cuts at: 0 122 1119
Size: 122 997
XmnI GAAnn'nnTTC
Cuts at: 0 745 1087 1119
Size: 745 342 32
Fragments arranged by size:
745 342 32
Enzymes that do cut:
AcuI AfeI AhdI AlwNI AvaI BamHI BanI BcgI
BfrBI BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspMI BsrDI
BstEII BstXI BstYI BtgI BtsI DraIII DrdI EaeI
EarI EciI Eco57MI EcoO109I EcoRV HaeII Hin4I MmeI
MscI MslI MspA1I NcoI NheI NsiI NspI PasI
PflMI PpiI PpuMI PstI RsrII SfcI SmaI SmlI
StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BmgBI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI EagI EcoICRI EcoNI
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NotI NruI PacI PciI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XhoI ZraI