Restriction map for
pF1KB8133
(Linear) (Six-base) MAPSORT of: KIBB8133.seq Check: 8297 from: 1 to: 1698
KIBB8133.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AatII G_ACGT'C
Cuts at: 0 1436 1698
Size: 1436 262
AccI GT'mk_AC
Cuts at: 0 41 1698
Size: 41 1657
Acc65I G'GTAC_C
Cuts at: 0 311 1244 1698
Size: 311 933 454
Fragments arranged by size:
933 454 311
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1536 1698
Size: 1536 162
AflIII A'CryG_T
Cuts at: 0 569 1698
Size: 569 1129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 37 1698
Size: 37 1661
AlwNI CAG_nnn'CTG
Cuts at: 0 225 261 285 1110 1572 1698
Size: 225 36 24 825 462 126
Fragments arranged by size:
825 462 225 126 36 24
ApaI G_GGCC'C
Cuts at: 0 67 1139 1698
Size: 67 1072 559
Fragments arranged by size:
1072 559 67
AvaI C'yCGr_G
Cuts at: 0 465 606 1015 1698
Size: 465 141 409 683
Fragments arranged by size:
683 465 409 141
BanI G'GyrC_C
Cuts at: 0 237 311 1033 1244 1644 1698
Size: 237 74 722 211 400 54
Fragments arranged by size:
722 400 237 211 74 54
BanII G_rGCy'C
Cuts at: 0 67 1139 1162 1377 1698
Size: 67 1072 23 215 321
Fragments arranged by size:
1072 321 215 67 23
BbeI G_GCGC'C
Cuts at: 0 1037 1698
Size: 1037 661
BbsI GAAGACnn'nnnn_
Cuts at: 0 1542 1698
Size: 1542 156
BbvCI CC'TCA_GC
Cuts at: 0 292 1698
Size: 292 1406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 759 793 1006 1040 1428 1462 1698
Size: 759 34 213 34 388 34 236
Fragments arranged by size:
759 388 236 213 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1459 1698
Size: 1459 239
BglII A'GATC_T
Cuts at: 0 47 350 1698
Size: 47 303 1348
Fragments arranged by size:
1348 303 47
Bme1580I G_kGCm'C
Cuts at: 0 67 182 242 1139 1698
Size: 67 115 60 897 559
Fragments arranged by size:
897 559 115 67 60
BmgBI CAC'GTC
Cuts at: 0 75 177 570 821 1191 1698
Size: 75 102 393 251 370 507
Fragments arranged by size:
507 393 370 251 102 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 417 426 1698
Size: 417 9 1272
Fragments arranged by size:
1272 417 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1391 1698
Size: 1391 307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 432 949 1624 1698
Size: 375 57 517 675 74
Fragments arranged by size:
675 517 375 74 57
Bpu10I CC'TnA_GC
Cuts at: 0 55 292 1698
Size: 55 237 1406
Fragments arranged by size:
1406 237 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1333 1698
Size: 1333 365
BsaI GGTCTCn'nnnn_
Cuts at: 0 586 964 1698
Size: 586 378 734
Fragments arranged by size:
734 586 378
BsaAI yAC'GTr
Cuts at: 0 1254 1698
Size: 1254 444
BsaBI GATnn'nnATC
Cuts at: 0 1096 1698
Size: 1096 602
BsaHI Gr'CG_yC
Cuts at: 0 1034 1433 1595 1698
Size: 1034 399 162 103
Fragments arranged by size:
1034 399 162 103
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 483 874 1336 1698
Size: 226 257 391 462 362
Fragments arranged by size:
462 391 362 257 226
BseYI C'CCAG_C
Cuts at: 0 189 1143 1698
Size: 189 954 555
Fragments arranged by size:
954 555 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 274 324 367 601 1698
Size: 271 3 50 43 234 1097
Fragments arranged by size:
1097 271 234 50 43 3
BsiEI CG_ry'CG
Cuts at: 0 71 1698
Size: 71 1627
Bsp1286I G_dGCh'C
Cuts at: 0 67 182 242 1139 1162 1377 1698
Size: 67 115 60 897 23 215 321
Fragments arranged by size:
897 321 215 115 67 60 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 623 800 1340 1698
Size: 623 177 540 358
Fragments arranged by size:
623 540 358 177
BsrFI r'CCGG_y
Cuts at: 0 805 1469 1698
Size: 805 664 229
Fragments arranged by size:
805 664 229
BssSI C'ACGA_G
Cuts at: 0 1525 1698
Size: 1525 173
BstAPI GCAn_nnn'nTGC
Cuts at: 0 261 1110 1572 1698
Size: 261 849 462 126
Fragments arranged by size:
849 462 261 126
BstEII G'GTnAC_C
Cuts at: 0 808 1698
Size: 808 890
BstYI r'GATC_y
Cuts at: 0 47 350 719 1106 1698
Size: 47 303 369 387 592
Fragments arranged by size:
592 387 369 303 47
BtgI C'CryG_G
Cuts at: 0 1127 1654 1698
Size: 1127 527 44
Fragments arranged by size:
1127 527 44
BtsI GCAGTG_nn'
Cuts at: 0 1029 1698
Size: 1029 669
DrdI GACnn_nn'nnGTC
Cuts at: 0 37 1698
Size: 37 1661
EaeI y'GGCC_r
Cuts at: 0 68 80 194 761 815 980 1289 1451
Size: 68 12 114 567 54 165 309 162
Cuts at: 1451 1511 1583 1698
Size: 60 72 115
Fragments arranged by size:
567 309 165 162 115 114 72 68
60 54 12
EagI C'GGCC_G
Cuts at: 0 68 1698
Size: 68 1630
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1477 1698
Size: 1477 221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 432 949 1536 1624 1698
Size: 375 57 517 587 88 74
Fragments arranged by size:
587 517 375 88 74 57
EcoO109I rG'GnC_Cy
Cuts at: 0 282 1135 1698
Size: 282 853 563
Fragments arranged by size:
853 563 282
EcoRV GAT'ATC
Cuts at: 0 633 1698
Size: 633 1065
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 918 1698
Size: 918 780
FspI TGC'GCA
Cuts at: 0 1385 1698
Size: 1385 313
FspAI rTGC'GCAy
Cuts at: 0 1385 1698
Size: 1385 313
HaeII r_GCGC'y
Cuts at: 0 1037 1617 1698
Size: 1037 580 81
Fragments arranged by size:
1037 580 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 667 699 1199 1231 1698
Size: 622 32 13 32 500 32 467
Fragments arranged by size:
622 500 467 32 32 32 13
HincII GTy'rAC
Cuts at: 0 696 1539 1698
Size: 696 843 159
Fragments arranged by size:
843 696 159
KasI G'GCGC_C
Cuts at: 0 1033 1698
Size: 1033 665
KpnI G_GTAC'C
Cuts at: 0 315 1248 1698
Size: 315 933 450
Fragments arranged by size:
933 450 315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 935 1698
Size: 935 763
MscI TGG'CCA
Cuts at: 0 1453 1513 1698
Size: 1453 60 185
Fragments arranged by size:
1453 185 60
MslI CAynn'nnrTG
Cuts at: 0 754 1219 1529 1698
Size: 754 465 310 169
Fragments arranged by size:
754 465 310 169
MspA1I CmG'CkG
Cuts at: 0 193 222 951 1457 1698
Size: 193 29 729 506 241
Fragments arranged by size:
729 506 241 193 29
NarI GG'CG_CC
Cuts at: 0 1034 1698
Size: 1034 664
NspI r_CATG'y
Cuts at: 0 399 966 1698
Size: 399 567 732
Fragments arranged by size:
732 567 399
PasI CC'CwG_GG
Cuts at: 0 996 1698
Size: 996 702
PflMI CCAn_nnn'nTGG
Cuts at: 0 203 678 1698
Size: 203 475 1020
Fragments arranged by size:
1020 475 203
PfoI T'CCnGG_A
Cuts at: 0 671 1427 1698
Size: 671 756 271
Fragments arranged by size:
756 671 271
PpuMI rG'GwC_Cy
Cuts at: 0 282 1698
Size: 282 1416
PspOMI G'GGCC_C
Cuts at: 0 63 1135 1698
Size: 63 1072 563
Fragments arranged by size:
1072 563 63
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 199 231 1698
Size: 199 32 1467
Fragments arranged by size:
1467 199 32
PstI C_TGCA'G
Cuts at: 0 772 1523 1698
Size: 772 751 175
Fragments arranged by size:
772 751 175
PvuII CAG'CTG
Cuts at: 0 951 1698
Size: 951 747
SexAI A'CCwGG_T
Cuts at: 0 161 278 1698
Size: 161 117 1420
Fragments arranged by size:
1420 161 117
SfcI C'TryA_G
Cuts at: 0 768 1519 1698
Size: 768 751 179
Fragments arranged by size:
768 751 179
SfoI GGC'GCC
Cuts at: 0 1035 1698
Size: 1035 663
SmlI C'TyrA_G
Cuts at: 0 1348 1698
Size: 1348 350
SphI G_CATG'C
Cuts at: 0 966 1698
Size: 966 732
StyI C'CwwG_G
Cuts at: 0 332 893 974 1698
Size: 332 561 81 724
Fragments arranged by size:
724 561 332 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 203 436 1135 1698
Size: 203 233 699 563
Fragments arranged by size:
699 563 233 203
Tth111I GACn'n_nGTC
Cuts at: 0 280 1189 1698
Size: 280 909 509
Fragments arranged by size:
909 509 280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1281 1698
Size: 1281 417
ZraI GAC'GTC
Cuts at: 0 1434 1698
Size: 1434 264
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AlfI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiEI
Bsp1286I BspMI BsrFI BssSI BstAPI BstEII BstYI BtgI
BtsI DrdI EaeI EagI EciI Eco57MI EcoO109I EcoRV
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NarI NspI PasI PflMI
PfoI PpuMI PspOMI PsrI PstI PvuII SexAI SfcI
SfoI SmlI SphI StyI TaqII Tth111I XcmI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AleI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BlpI BmtI BsaWI BsaXI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EarI EcoICRI EcoNI EcoRI FseI HindIII
HpaI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TatI TstI
XbaI XhoI XmaI XmnI