Restriction map for
pF1KB8132
(Linear) (Six-base) MAPSORT of: KIBB8132.seq Check: 4162 from: 1 to: 1530
KIBB8132.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AccI GT'mk_AC
Cuts at: 0 167 1530
Size: 167 1363
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 736 1338 1530
Size: 667 69 602 192
Fragments arranged by size:
667 602 192 69
AfeI AGC'GCT
Cuts at: 0 861 1318 1530
Size: 861 457 212
Fragments arranged by size:
861 457 212
AhdI GACnn_n'nnGTC
Cuts at: 0 1023 1530
Size: 1023 507
AlwNI CAG_nnn'CTG
Cuts at: 0 198 231 264 291 645 675 1530
Size: 198 33 33 27 354 30 855
Fragments arranged by size:
855 354 198 33 33 30 27
ApaI G_GGCC'C
Cuts at: 0 188 1198 1429 1530
Size: 188 1010 231 101
Fragments arranged by size:
1010 231 188 101
ApoI r'AATT_y
Cuts at: 0 160 1530
Size: 160 1370
AvaI C'yCGr_G
Cuts at: 0 100 1120 1144 1421 1530
Size: 100 1020 24 277 109
Fragments arranged by size:
1020 277 109 100 24
BanI G'GyrC_C
Cuts at: 0 537 921 958 1417 1530
Size: 537 384 37 459 113
Fragments arranged by size:
537 459 384 113 37
BanII G_rGCy'C
Cuts at: 0 40 188 418 430 497 505 1198 1429
Size: 40 148 230 12 67 8 693 231
Cuts at: 1429 1530
Size: 101
Fragments arranged by size:
693 231 230 148 101 67 40 12
8
BbeI G_GCGC'C
Cuts at: 0 925 1421 1530
Size: 925 496 109
Fragments arranged by size:
925 496 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 1348 1387 1530
Size: 1348 39 143
Fragments arranged by size:
1348 143 39
BbvCI CC'TCA_GC
Cuts at: 0 668 1530
Size: 668 862
BglI GCCn_nnn'nGGC
Cuts at: 0 1148 1307 1530
Size: 1148 159 223
Fragments arranged by size:
1148 223 159
Bme1580I G_kGCm'C
Cuts at: 0 188 961 1090 1198 1429 1530
Size: 188 773 129 108 231 101
Fragments arranged by size:
773 231 188 129 108 101
BmrI ACTGGGnnnn_n'
Cuts at: 0 1108 1530
Size: 1108 422
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 264 1047 1170 1530
Size: 264 783 123 360
Fragments arranged by size:
783 360 264 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 286 301 759 1201 1530
Size: 154 132 15 458 442 329
Fragments arranged by size:
458 442 329 154 132 15
Bpu10I CC'TnA_GC
Cuts at: 0 668 905 1530
Size: 668 237 625
Fragments arranged by size:
668 625 237
BsaI GGTCTCn'nnnn_
Cuts at: 0 97 1530
Size: 97 1433
BsaHI Gr'CG_yC
Cuts at: 0 922 935 1151 1418 1530
Size: 922 13 216 267 112
Fragments arranged by size:
922 267 216 112 13
BsaWI w'CCGG_w
Cuts at: 0 564 725 797 1039 1530
Size: 564 161 72 242 491
Fragments arranged by size:
564 491 242 161 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 757 787 1530
Size: 4 30 723 30 743
Fragments arranged by size:
743 723 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 603 721 892 982 985 1530
Size: 603 118 171 90 3 545
Fragments arranged by size:
603 545 171 118 90 3
BseYI C'CCAG_C
Cuts at: 0 42 411 505 523 758 862 1410 1530
Size: 42 369 94 18 235 104 548 120
Fragments arranged by size:
548 369 235 120 104 94 42 18
BsiEI CG_ry'CG
Cuts at: 0 1311 1530
Size: 1311 219
BsiHKAI G_wGCw'C
Cuts at: 0 40 497 1292 1376 1530
Size: 40 457 795 84 154
Fragments arranged by size:
795 457 154 84 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1158 1530
Size: 1158 372
Bsp1286I G_dGCh'C
Cuts at: 0 40 188 418 430 497 505 961 1090
Size: 40 148 230 12 67 8 456 129
Cuts at: 1090 1198 1292 1376 1429 1530
Size: 108 94 84 53 101
Fragments arranged by size:
456 230 148 129 108 101 94 84
67 53 40 12 8
BspEI T'CCGG_A
Cuts at: 0 564 1530
Size: 564 966
BssHII G'CGCG_C
Cuts at: 0 244 1530
Size: 244 1286
BssSI C'ACGA_G
Cuts at: 0 1387 1530
Size: 1387 143
BstXI CCAn_nnnn'nTGG
Cuts at: 0 517 1530
Size: 517 1013
BstYI r'GATC_y
Cuts at: 0 54 530 813 1530
Size: 54 476 283 717
Fragments arranged by size:
717 476 283 54
Bsu36I CC'TnA_GG
Cuts at: 0 239 1530
Size: 239 1291
BtgI C'CryG_G
Cuts at: 0 890 1224 1302 1530
Size: 890 334 78 228
Fragments arranged by size:
890 334 228 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 461 1530
Size: 461 1069
EaeI y'GGCC_r
Cuts at: 0 1308 1530
Size: 1308 222
EagI C'GGCC_G
Cuts at: 0 1308 1530
Size: 1308 222
EarI CTCTTCn'nnn_
Cuts at: 0 389 608 712 971 1530
Size: 389 219 104 259 559
Fragments arranged by size:
559 389 259 219 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1487 1530
Size: 1487 43
EcoICRI GAG'CTC
Cuts at: 0 38 495 1530
Size: 38 457 1035
Fragments arranged by size:
1035 457 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 286 301 667 736 759 1201 1338
Size: 154 132 15 366 69 23 442 137
Cuts at: 1338 1530
Size: 192
Fragments arranged by size:
442 366 192 154 137 132 69 23
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 30 370 1530
Size: 30 340 1160
Fragments arranged by size:
1160 340 30
EcoO109I rG'GnC_Cy
Cuts at: 0 119 185 375 513 663 993 1008 1194
Size: 119 66 190 138 150 330 15 186
Cuts at: 1194 1195 1425 1426 1530
Size: 1 230 1 104
Fragments arranged by size:
330 230 190 186 150 138 119 104
66 15 1 1
HaeII r_GCGC'y
Cuts at: 0 863 925 1320 1421 1530
Size: 863 62 395 101 109
Fragments arranged by size:
863 395 109 101 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 757 789 937 969 1009 1041 1530
Size: 757 32 148 32 40 32 489
Fragments arranged by size:
757 489 148 40 32 32 32
KasI G'GCGC_C
Cuts at: 0 921 1417 1530
Size: 921 496 113
Fragments arranged by size:
921 496 113
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1052 1530
Size: 1052 478
MspA1I CmG'CkG
Cuts at: 0 892 1226 1530
Size: 892 334 304
Fragments arranged by size:
892 334 304
NarI GG'CG_CC
Cuts at: 0 922 1418 1530
Size: 922 496 112
Fragments arranged by size:
922 496 112
NcoI C'CATG_G
Cuts at: 0 1302 1530
Size: 1302 228
NruI TCG'CGA
Cuts at: 0 499 1530
Size: 499 1031
PasI CC'CwG_GG
Cuts at: 0 320 928 1198 1530
Size: 320 608 270 332
Fragments arranged by size:
608 332 320 270
PflMI CCAn_nnn'nTGG
Cuts at: 0 198 1530
Size: 198 1332
PfoI T'CCnGG_A
Cuts at: 0 155 389 1530
Size: 155 234 1141
Fragments arranged by size:
1141 234 155
PpuMI rG'GwC_Cy
Cuts at: 0 119 663 1008 1530
Size: 119 544 345 522
Fragments arranged by size:
544 522 345 119
PspOMI G'GGCC_C
Cuts at: 0 184 1194 1425 1530
Size: 184 1010 231 105
Fragments arranged by size:
1010 231 184 105
PspXI vC'TCGA_Gb
Cuts at: 0 1144 1530
Size: 1144 386
SacI G_AGCT'C
Cuts at: 0 40 497 1530
Size: 40 457 1033
Fragments arranged by size:
1033 457 40
SacII CC_GC'GG
Cuts at: 0 893 1227 1530
Size: 893 334 303
Fragments arranged by size:
893 334 303
SapI GCTCTTCn'nnn_
Cuts at: 0 389 712 971 1530
Size: 389 323 259 559
Fragments arranged by size:
559 389 323 259
SfoI GGC'GCC
Cuts at: 0 923 1419 1530
Size: 923 496 111
Fragments arranged by size:
923 496 111
SmaI CCC'GGG
Cuts at: 0 1423 1530
Size: 1423 107
SmlI C'TyrA_G
Cuts at: 0 1144 1530
Size: 1144 386
StuI AGG'CCT
Cuts at: 0 1261 1530
Size: 1261 269
StyI C'CwwG_G
Cuts at: 0 251 516 1302 1530
Size: 251 265 786 228
Fragments arranged by size:
786 265 251 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 312 625 854 1530
Size: 312 313 229 676
Fragments arranged by size:
676 313 312 229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1300 1530
Size: 1300 230
XhoI C'TCGA_G
Cuts at: 0 1144 1530
Size: 1144 386
XmaI C'CCGG_G
Cuts at: 0 1421 1530
Size: 1421 109
XmnI GAAnn'nnTTC
Cuts at: 0 1345 1530
Size: 1345 185
Enzymes that do cut:
AccI AcuI AfeI AhdI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BssHII BssSI
BstXI BstYI Bsu36I BtgI BtgZI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MmeI MspA1I NarI NcoI NruI PasI PflMI PfoI
PpuMI PspOMI PspXI SacI SacII SapI SfoI SmaI
SmlI StuI StyI TaqII XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BpuEI BsaAI BsaBI BsgI BsiWI BsmI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI BstEII
BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI MslI NaeI NdeI NgoMIV
NheI NotI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PsrI PstI PvuI PvuII RsrII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI ZraI