(Linear) (Six-base) MAPSORT of: KIBB8131.seq  Check: 9766  from: 1  to: 549



KIBB8131.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AccI GT'mk_AC

Cuts at:      0     242     549 
   Size:        242     307
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     549 
   Size:        425     124
 
AvaI C'yCGr_G

Cuts at:      0      30     549 
   Size:         30     519
 
BanI G'GyrC_C

Cuts at:      0      76     178     549 
   Size:         76     102     371

  Fragments arranged by size: 

                 371     102      76
 
BanII G_rGCy'C

Cuts at:      0      31     399     549 
   Size:         31     368     150

  Fragments arranged by size: 

                 368     150      31
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      66     100     111     145     451     485     549 
   Size:         66      34      11      34     306      34      64

  Fragments arranged by size: 

                 306      66      64      34      34      34      11
 
BlpI GC'TnA_GC

Cuts at:      0     503     549 
   Size:        503      46
 
Bme1580I G_kGCm'C

Cuts at:      0     181     549 
   Size:        181     368
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     549 
   Size:        178     371
 
BseYI C'CCAG_C

Cuts at:      0      24     549 
   Size:         24     525
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     549 
   Size:         82     467
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     181     399     549 
   Size:         31     150     218     150

  Fragments arranged by size: 

                 218     150     150      31
 
BsrFI r'CCGG_y

Cuts at:      0      73     549 
   Size:         73     476
 
BsrGI T'GTAC_A

Cuts at:      0     209     549 
   Size:        209     340
 
BstYI r'GATC_y

Cuts at:      0     161     549 
   Size:        161     388
 
EaeI y'GGCC_r

Cuts at:      0     222     549 
   Size:        222     327
 
EarI CTCTTCn'nnn_

Cuts at:      0     401     549 
   Size:        401     148
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      39     549 
   Size:         39     510
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     425     549 
   Size:        178     247     124

  Fragments arranged by size: 

                 247     178     124
 
EcoO109I rG'GnC_Cy

Cuts at:      0     204     549 
   Size:        204     345
 
HincII GTy'rAC

Cuts at:      0     255     549 
   Size:        255     294
 
MslI CAynn'nnrTG

Cuts at:      0     304     549 
   Size:        304     245
 
NspI r_CATG'y

Cuts at:      0     539     549 
   Size:        539      10
 
PfoI T'CCnGG_A

Cuts at:      0     164     269     549 
   Size:        164     105     280

  Fragments arranged by size: 

                 280     164     105
 
PpuMI rG'GwC_Cy

Cuts at:      0     204     549 
   Size:        204     345
 
SfcI C'TryA_G

Cuts at:      0     243     549 
   Size:        243     306
 
TatI w'GTAC_w

Cuts at:      0       8     209     549 
   Size:          8     201     340

  Fragments arranged by size: 

                 340     201       8
 

 Enzymes that do cut:

     AccI     AcuI     AvaI     BanI    BanII     BcgI     BlpI Bme1580I
     BpmI    BseYI     BsgI Bsp1286I    BsrFI    BsrGI    BstYI     EaeI
     EarI     EciI  Eco57MI EcoO109I   HincII     MslI     NspI     PfoI
    PpuMI     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI