Restriction map for
pF1KB8127
(Linear) (Six-base) MAPSORT of: KIBB8127.seq Check: 9223 from: 1 to: 1353
KIBB8127.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AatII G_ACGT'C
Cuts at: 0 149 1353
Size: 149 1204
Acc65I G'GTAC_C
Cuts at: 0 479 1353
Size: 479 874
AflIII A'CryG_T
Cuts at: 0 1235 1353
Size: 1235 118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1222 1254 1353
Size: 1222 32 99
Fragments arranged by size:
1222 99 32
ApaI G_GGCC'C
Cuts at: 0 1057 1353
Size: 1057 296
ApoI r'AATT_y
Cuts at: 0 338 1353
Size: 338 1015
AvaI C'yCGr_G
Cuts at: 0 5 97 185 212 1213 1353
Size: 5 92 88 27 1001 140
Fragments arranged by size:
1001 140 92 88 27 5
BamHI G'GATC_C
Cuts at: 0 1016 1353
Size: 1016 337
BanI G'GyrC_C
Cuts at: 0 479 1339 1353
Size: 479 860 14
Fragments arranged by size:
860 479 14
BanII G_rGCy'C
Cuts at: 0 451 1057 1127 1292 1353
Size: 451 606 70 165 61
Fragments arranged by size:
606 451 165 70 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 353 865 1353
Size: 353 512 488
Fragments arranged by size:
512 488 353
BbvCI CC'TCA_GC
Cuts at: 0 1104 1353
Size: 1104 249
BglI GCCn_nnn'nGGC
Cuts at: 0 38 101 1353
Size: 38 63 1252
Fragments arranged by size:
1252 63 38
Bme1580I G_kGCm'C
Cuts at: 0 133 1057 1342 1353
Size: 133 924 285 11
Fragments arranged by size:
924 285 133 11
BmgBI CAC'GTC
Cuts at: 0 1236 1353
Size: 1236 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 318 1353
Size: 318 1035
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1106 1353
Size: 1106 247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1353
Size: 481 872
Bpu10I CC'TnA_GC
Cuts at: 0 1104 1353
Size: 1104 249
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1353
Size: 559 794
BsaI GGTCTCn'nnnn_
Cuts at: 0 920 1353
Size: 920 433
BsaHI Gr'CG_yC
Cuts at: 0 146 1353
Size: 146 1207
BsaWI w'CCGG_w
Cuts at: 0 632 1353
Size: 632 721
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1222 1252 1353
Size: 1222 30 101
Fragments arranged by size:
1222 101 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 305 308 1143 1353
Size: 127 178 3 835 210
Fragments arranged by size:
835 210 178 127 3
BseYI C'CCAG_C
Cuts at: 0 27 424 1316 1353
Size: 27 397 892 37
Fragments arranged by size:
892 397 37 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 637 1353
Size: 517 120 716
Fragments arranged by size:
716 517 120
BsiHKAI G_wGCw'C
Cuts at: 0 176 1292 1353
Size: 176 1116 61
Fragments arranged by size:
1116 176 61
Bsp1286I G_dGCh'C
Cuts at: 0 133 176 451 1057 1127 1292 1342 1353
Size: 133 43 275 606 70 165 50 11
Fragments arranged by size:
606 275 165 133 70 50 43 11
BspHI T'CATG_A
Cuts at: 0 342 806 878 1127 1353
Size: 342 464 72 249 226
Fragments arranged by size:
464 342 249 226 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1106 1353
Size: 1106 247
BsrBI CCG'CTC
Cuts at: 0 825 1353
Size: 825 528
BsrFI r'CCGG_y
Cuts at: 0 811 1353
Size: 811 542
BstXI CCAn_nnnn'nTGG
Cuts at: 0 870 1353
Size: 870 483
BstYI r'GATC_y
Cuts at: 0 1016 1353
Size: 1016 337
BtgI C'CryG_G
Cuts at: 0 790 863 1048 1353
Size: 790 73 185 305
Fragments arranged by size:
790 305 185 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 405 753 936 1353
Size: 405 348 183 417
Fragments arranged by size:
417 405 348 183
BtsI GCAGTG_nn'
Cuts at: 0 942 1353
Size: 942 411
EaeI y'GGCC_r
Cuts at: 0 710 845 1034 1133 1310 1353
Size: 710 135 189 99 177 43
Fragments arranged by size:
710 189 177 135 99 43
EcoICRI GAG'CTC
Cuts at: 0 1290 1353
Size: 1290 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1353
Size: 481 872
EcoO109I rG'GnC_Cy
Cuts at: 0 1053 1054 1353
Size: 1053 1 299
Fragments arranged by size:
1053 299 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 165 1266 1353
Size: 165 1101 87
Fragments arranged by size:
1101 165 87
KpnI G_GTAC'C
Cuts at: 0 483 1353
Size: 483 870
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1353
Size: 260 1093
MscI TGG'CCA
Cuts at: 0 847 1036 1135 1353
Size: 847 189 99 218
Fragments arranged by size:
847 218 189 99
MslI CAynn'nnrTG
Cuts at: 0 703 1069 1353
Size: 703 366 284
Fragments arranged by size:
703 366 284
MspA1I CmG'CkG
Cuts at: 0 27 72 317 690 1121 1353
Size: 27 45 245 373 431 232
Fragments arranged by size:
431 373 245 232 45 27
PasI CC'CwG_GG
Cuts at: 0 327 1353
Size: 327 1026
PflMI CCAn_nnn'nTGG
Cuts at: 0 843 1353
Size: 843 510
PfoI T'CCnGG_A
Cuts at: 0 458 869 1019 1353
Size: 458 411 150 334
Fragments arranged by size:
458 411 334 150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1222 1254 1353
Size: 1222 32 99
Fragments arranged by size:
1222 99 32
PshAI GACnn'nnGTC
Cuts at: 0 865 1353
Size: 865 488
PspOMI G'GGCC_C
Cuts at: 0 1053 1353
Size: 1053 300
PspXI vC'TCGA_Gb
Cuts at: 0 1213 1353
Size: 1213 140
PstI C_TGCA'G
Cuts at: 0 26 896 1013 1353
Size: 26 870 117 340
Fragments arranged by size:
870 340 117 26
PvuII CAG'CTG
Cuts at: 0 27 72 690 1353
Size: 27 45 618 663
Fragments arranged by size:
663 618 45 27
SacI G_AGCT'C
Cuts at: 0 1292 1353
Size: 1292 61
SfcI C'TryA_G
Cuts at: 0 22 830 892 1009 1353
Size: 22 808 62 117 344
Fragments arranged by size:
808 344 117 62 22
SmaI CCC'GGG
Cuts at: 0 7 187 214 1353
Size: 7 180 27 1139
Fragments arranged by size:
1139 180 27 7
SmlI C'TyrA_G
Cuts at: 0 574 1213 1353
Size: 574 639 140
Fragments arranged by size:
639 574 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 383 1006 1330 1353
Size: 383 623 324 23
Fragments arranged by size:
623 383 324 23
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 906 1068 1353
Size: 906 162 285
Fragments arranged by size:
906 285 162
XhoI C'TCGA_G
Cuts at: 0 1213 1353
Size: 1213 140
XmaI C'CCGG_G
Cuts at: 0 5 185 212 1353
Size: 5 180 27 1141
Fragments arranged by size:
1141 180 27 5
ZraI GAC'GTC
Cuts at: 0 147 1353
Size: 147 1206
Enzymes that do cut:
AatII Acc65I AflIII AloI ApaI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrFI BstXI BstYI BtgI BtgZI BtsI EaeI EcoICRI
Eco57MI EcoO109I FalI KpnI MmeI MscI MslI MspA1I
PasI PflMI PfoI PpiI PshAI PspOMI PspXI PstI
PvuII SacI SfcI SmaI SmlI TaqII XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspEI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpuMI PsiI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TatI TstI Tth111I
XbaI XmnI