Restriction map for
pF1KB8125
(Linear) (Six-base) MAPSORT of: KIBB8125.seq Check: 9840 from: 1 to: 1365
KIBB8125.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AatII G_ACGT'C
Cuts at: 0 632 1365
Size: 632 733
AccI GT'mk_AC
Cuts at: 0 567 1256 1365
Size: 567 689 109
Fragments arranged by size:
689 567 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1310 1365
Size: 142 1168 55
Fragments arranged by size:
1168 142 55
AflII C'TTAA_G
Cuts at: 0 864 1365
Size: 864 501
AflIII A'CryG_T
Cuts at: 0 768 1365
Size: 768 597
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1310 1342 1365
Size: 1310 32 23
Fragments arranged by size:
1310 32 23
AlwNI CAG_nnn'CTG
Cuts at: 0 51 1365
Size: 51 1314
ApoI r'AATT_y
Cuts at: 0 18 54 280 559 1185 1365
Size: 18 36 226 279 626 180
Fragments arranged by size:
626 279 226 180 36 18
AvaI C'yCGr_G
Cuts at: 0 9 1365
Size: 9 1356
AvrII C'CTAG_G
Cuts at: 0 820 1365
Size: 820 545
BanII G_rGCy'C
Cuts at: 0 208 1365
Size: 208 1157
BbsI GAAGACnn'nnnn_
Cuts at: 0 1205 1365
Size: 1205 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 641 675 1365
Size: 641 34 690
Fragments arranged by size:
690 641 34
BclI T'GATC_A
Cuts at: 0 1149 1365
Size: 1149 216
Bme1580I G_kGCm'C
Cuts at: 0 173 1365
Size: 173 1192
BmrI ACTGGGnnnn_n'
Cuts at: 0 60 1365
Size: 60 1305
BmtI G_CTAG'C
Cuts at: 0 1303 1365
Size: 1303 62
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 908 1260 1365
Size: 908 352 105
Fragments arranged by size:
908 352 105
Bpu10I CC'TnA_GC
Cuts at: 0 207 1365
Size: 207 1158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 1365
Size: 363 1002
BsaHI Gr'CG_yC
Cuts at: 0 629 1365
Size: 629 736
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 947 977 1365
Size: 947 30 388
Fragments arranged by size:
947 388 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 655 1365
Size: 655 710
BseYI C'CCAG_C
Cuts at: 0 827 1005 1365
Size: 827 178 360
Fragments arranged by size:
827 360 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 418 1365
Size: 157 261 947
Fragments arranged by size:
947 261 157
BsiHKAI G_wGCw'C
Cuts at: 0 208 1365
Size: 208 1157
Bsp1286I G_dGCh'C
Cuts at: 0 173 208 1365
Size: 173 35 1157
Fragments arranged by size:
1157 173 35
BspHI T'CATG_A
Cuts at: 0 257 632 1365
Size: 257 375 733
Fragments arranged by size:
733 375 257
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 1365
Size: 113 1252
BssHII G'CGCG_C
Cuts at: 0 141 143 1365
Size: 141 2 1222
Fragments arranged by size:
1222 141 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 168 405 1365
Size: 168 237 960
Fragments arranged by size:
960 237 168
BstEII G'GTnAC_C
Cuts at: 0 1216 1365
Size: 1216 149
BstYI r'GATC_y
Cuts at: 0 221 1365
Size: 221 1144
BstZ17I GTA'TAC
Cuts at: 0 568 1365
Size: 568 797
Bsu36I CC'TnA_GG
Cuts at: 0 380 1365
Size: 380 985
BtgI C'CryG_G
Cuts at: 0 131 1203 1346 1365
Size: 131 1072 143 19
Fragments arranged by size:
1072 143 131 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 228 1365
Size: 228 1137
BtsI GCAGTG_nn'
Cuts at: 0 1127 1365
Size: 1127 238
DraIII CAC_nnn'GTG
Cuts at: 0 168 1365
Size: 168 1197
EaeI y'GGCC_r
Cuts at: 0 432 617 1206 1365
Size: 432 185 589 159
Fragments arranged by size:
589 432 185 159
EarI CTCTTCn'nnn_
Cuts at: 0 196 787 1286 1365
Size: 196 591 499 79
Fragments arranged by size:
591 499 196 79
EcoICRI GAG'CTC
Cuts at: 0 206 1365
Size: 206 1159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1310 1365
Size: 142 1168 55
Fragments arranged by size:
1168 142 55
EcoO109I rG'GnC_Cy
Cuts at: 0 824 1341 1365
Size: 824 517 24
Fragments arranged by size:
824 517 24
EcoRI G'AATT_C
Cuts at: 0 18 54 559 1185 1365
Size: 18 36 505 626 180
Fragments arranged by size:
626 505 180 36 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 507 798 1365
Size: 507 291 567
Fragments arranged by size:
567 507 291
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 206 238 1365
Size: 206 32 1127
Fragments arranged by size:
1127 206 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 858 963 1365
Size: 83 775 105 402
Fragments arranged by size:
775 402 105 83
MscI TGG'CCA
Cuts at: 0 434 1208 1365
Size: 434 774 157
Fragments arranged by size:
774 434 157
NcoI C'CATG_G
Cuts at: 0 1203 1346 1365
Size: 1203 143 19
Fragments arranged by size:
1203 143 19
NheI G'CTAG_C
Cuts at: 0 1299 1365
Size: 1299 66
NspI r_CATG'y
Cuts at: 0 154 772 1285 1365
Size: 154 618 513 80
Fragments arranged by size:
618 513 154 80
PciI A'CATG_T
Cuts at: 0 768 1365
Size: 768 597
PpuMI rG'GwC_Cy
Cuts at: 0 1341 1365
Size: 1341 24
PshAI GACnn'nnGTC
Cuts at: 0 116 1365
Size: 116 1249
SacI G_AGCT'C
Cuts at: 0 208 1365
Size: 208 1157
SanDI GG'GwC_CC
Cuts at: 0 1341 1365
Size: 1341 24
SapI GCTCTTCn'nnn_
Cuts at: 0 196 1365
Size: 196 1169
SexAI A'CCwGG_T
Cuts at: 0 581 1365
Size: 581 784
SmlI C'TyrA_G
Cuts at: 0 342 864 1365
Size: 342 522 501
Fragments arranged by size:
522 501 342
SspI AAT'ATT
Cuts at: 0 273 456 1365
Size: 273 183 909
Fragments arranged by size:
909 273 183
StyI C'CwwG_G
Cuts at: 0 749 820 1203 1346 1365
Size: 749 71 383 143 19
Fragments arranged by size:
749 383 143 71 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 1365
Size: 112 1253
TatI w'GTAC_w
Cuts at: 0 836 1365
Size: 836 529
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 947 979 1365
Size: 947 32 386
Fragments arranged by size:
947 386 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 747 1353 1365
Size: 747 606 12
Fragments arranged by size:
747 606 12
XmnI GAAnn'nnTTC
Cuts at: 0 490 1365
Size: 490 875
ZraI GAC'GTC
Cuts at: 0 630 1365
Size: 630 735
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AloI AlwNI ApoI
AvaI AvrII BanII BbsI BcgI BclI Bme1580I BmrI
BmtI BplI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BssHII BstAPI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I MmeI
MscI NcoI NheI NspI PciI PpuMI PshAI SacI
SanDI SapI SexAI SmlI SspI StyI TaqII TatI
TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI AleI AlfI
ApaI ApaLI AscI AseI AsiSI BaeI BamHI BanI
BbeI BbvCI BciVI BfrBI BglI BglII BlpI BmgBI
BpmI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssSI BstBI
BstXI ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MslI MspA1I NaeI NarI
NdeI NgoMIV NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI