Restriction map for
pF1KB8123
(Linear) (Six-base) MAPSORT of: KIBB8123.seq Check: 3363 from: 1 to: 1551
KIBB8123.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 435 1551
Size: 435 1116
AatII G_ACGT'C
Cuts at: 0 580 1551
Size: 580 971
AccI GT'mk_AC
Cuts at: 0 348 854 1543 1551
Size: 348 506 689 8
Fragments arranged by size:
689 506 348 8
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 1310 1551
Size: 767 543 241
Fragments arranged by size:
767 543 241
ApoI r'AATT_y
Cuts at: 0 38 1303 1351 1402 1551
Size: 38 1265 48 51 149
Fragments arranged by size:
1265 149 51 48 38
AseI AT'TA_AT
Cuts at: 0 332 1551
Size: 332 1219
AvaI C'yCGr_G
Cuts at: 0 875 1551
Size: 875 676
BanI G'GyrC_C
Cuts at: 0 490 957 1551
Size: 490 467 594
Fragments arranged by size:
594 490 467
BanII G_rGCy'C
Cuts at: 0 937 1551
Size: 937 614
BbsI GAAGACnn'nnnn_
Cuts at: 0 189 755 1551
Size: 189 566 796
Fragments arranged by size:
796 566 189
BciVI GTATCCnnnnn_n'
Cuts at: 0 1178 1551
Size: 1178 373
BfrBI ATG'CAT
Cuts at: 0 1204 1551
Size: 1204 347
Bme1580I G_kGCm'C
Cuts at: 0 662 1551
Size: 662 889
BmrI ACTGGGnnnn_n'
Cuts at: 0 731 1131 1551
Size: 731 400 420
Fragments arranged by size:
731 420 400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 566 877 947 1551
Size: 404 162 311 70 604
Fragments arranged by size:
604 404 311 162 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 1018 1551
Size: 609 409 533
Fragments arranged by size:
609 533 409
BsaHI Gr'CG_yC
Cuts at: 0 577 1551
Size: 577 974
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 377 407 483 513 1087 1117 1551
Size: 377 30 76 30 574 30 434
Fragments arranged by size:
574 434 377 76 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 236 486 491 1551
Size: 37 199 250 5 1060
Fragments arranged by size:
1060 250 199 37 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 702 1099 1551
Size: 70 632 397 452
Fragments arranged by size:
632 452 397 70
BsiHKAI G_wGCw'C
Cuts at: 0 426 471 1551
Size: 426 45 1080
Fragments arranged by size:
1080 426 45
BsmI GAATG_Cn'
Cuts at: 0 384 690 1433 1551
Size: 384 306 743 118
Fragments arranged by size:
743 384 306 118
BsmBI CGTCTCn'nnnn_
Cuts at: 0 584 1551
Size: 584 967
Bsp1286I G_dGCh'C
Cuts at: 0 426 471 662 937 1551
Size: 426 45 191 275 614
Fragments arranged by size:
614 426 275 191 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 435 1055 1551
Size: 435 620 496
Fragments arranged by size:
620 496 435
BsrBI CCG'CTC
Cuts at: 0 277 1551
Size: 277 1274
BsrDI GCAATG_nn'
Cuts at: 0 698 1551
Size: 698 853
BssSI C'ACGA_G
Cuts at: 0 1527 1551
Size: 1527 24
BstEII G'GTnAC_C
Cuts at: 0 58 171 1551
Size: 58 113 1380
Fragments arranged by size:
1380 113 58
BstXI CCAn_nnnn'nTGG
Cuts at: 0 487 1551
Size: 487 1064
BstYI r'GATC_y
Cuts at: 0 1028 1551
Size: 1028 523
BstZ17I GTA'TAC
Cuts at: 0 1544 1551
Size: 1544 7
Bsu36I CC'TnA_GG
Cuts at: 0 928 1551
Size: 928 623
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 618 1551
Size: 618 933
BtsI GCAGTG_nn'
Cuts at: 0 492 1428 1551
Size: 492 936 123
Fragments arranged by size:
936 492 123
DraI TTT'AAA
Cuts at: 0 416 1551
Size: 416 1135
EaeI y'GGCC_r
Cuts at: 0 571 1551
Size: 571 980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 566 767 877 947 1310 1551
Size: 404 162 201 110 70 363 241
Fragments arranged by size:
404 363 241 201 162 110 70
EcoO109I rG'GnC_Cy
Cuts at: 0 1098 1551
Size: 1098 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 541 573 1551
Size: 541 32 978
Fragments arranged by size:
978 541 32
HincII GTy'rAC
Cuts at: 0 759 1551
Size: 759 792
MfeI C'AATT_G
Cuts at: 0 1238 1551
Size: 1238 313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1379 1551
Size: 1379 172
MslI CAynn'nnrTG
Cuts at: 0 106 334 558 703 1551
Size: 106 228 224 145 848
Fragments arranged by size:
848 228 224 145 106
NsiI A_TGCA'T
Cuts at: 0 1206 1551
Size: 1206 345
NspI r_CATG'y
Cuts at: 0 467 1208 1551
Size: 467 741 343
Fragments arranged by size:
741 467 343
PfoI T'CCnGG_A
Cuts at: 0 113 893 1551
Size: 113 780 658
Fragments arranged by size:
780 658 113
PpuMI rG'GwC_Cy
Cuts at: 0 1098 1551
Size: 1098 453
PstI C_TGCA'G
Cuts at: 0 53 1551
Size: 53 1498
SfcI C'TryA_G
Cuts at: 0 49 316 1551
Size: 49 267 1235
Fragments arranged by size:
1235 267 49
SmaI CCC'GGG
Cuts at: 0 877 1551
Size: 877 674
SmlI C'TyrA_G
Cuts at: 0 588 1033 1551
Size: 588 445 518
Fragments arranged by size:
588 518 445
SspI AAT'ATT
Cuts at: 0 810 1551
Size: 810 741
StyI C'CwwG_G
Cuts at: 0 263 1454 1551
Size: 263 1191 97
Fragments arranged by size:
1191 263 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1070 1551
Size: 1070 481
TatI w'GTAC_w
Cuts at: 0 905 1103 1551
Size: 905 198 448
Fragments arranged by size:
905 448 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 377 409 416 448 483 515 1551
Size: 377 32 7 32 35 32 1036
Fragments arranged by size:
1036 377 35 32 32 32 7
XbaI T'CTAG_A
Cuts at: 0 1170 1551
Size: 1170 381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 590 971 1551
Size: 590 381 580
Fragments arranged by size:
590 580 381
XmaI C'CCGG_G
Cuts at: 0 875 1551
Size: 875 676
XmnI GAAnn'nnTTC
Cuts at: 0 362 1000 1551
Size: 362 638 551
Fragments arranged by size:
638 551 362
ZraI GAC'GTC
Cuts at: 0 578 1551
Size: 578 973
Enzymes that do cut:
AarI AatII AccI AcuI ApoI AseI AvaI BanI
BanII BbsI BciVI BfrBI Bme1580I BmrI BpmI BpuEI
BsaHI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BssSI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI DraI EaeI Eco57MI EcoO109I Hin4I
HincII MfeI MmeI MslI NsiI NspI PfoI PpuMI
PstI SfcI SmaI SmlI SspI StyI TaqII TatI
TstI XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BglI BglII
BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaWI BseYI BsiEI BsiWI BspEI BspHI BsrFI BsrGI
BssHII BstAPI BstBI BtgI ClaI CspCI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XhoI