Restriction map for
pF1KB8122
(Linear) (Six-base) MAPSORT of: KIBB8122.seq Check: 1507 from: 1 to: 642
KIBB8122.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AclI AA'CG_TT
Cuts at: 0 357 642
Size: 357 285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 642
Size: 78 564
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 148 642
Size: 148 494
BanI G'GyrC_C
Cuts at: 0 501 642
Size: 501 141
BanII G_rGCy'C
Cuts at: 0 68 589 642
Size: 68 521 53
Fragments arranged by size:
521 68 53
BfrBI ATG'CAT
Cuts at: 0 177 642
Size: 177 465
Bme1580I G_kGCm'C
Cuts at: 0 506 642
Size: 506 136
BmrI ACTGGGnnnn_n'
Cuts at: 0 295 642
Size: 295 347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 232 484 642
Size: 127 105 252 158
Fragments arranged by size:
252 158 127 105
Bpu10I CC'TnA_GC
Cuts at: 0 327 642
Size: 327 315
BsaI GGTCTCn'nnnn_
Cuts at: 0 276 642
Size: 276 366
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 400 403 406 424 427 481 642
Size: 184 216 3 3 18 3 54 161
Fragments arranged by size:
216 184 161 54 18 3 3 3
BseYI C'CCAG_C
Cuts at: 0 101 198 642
Size: 101 97 444
Fragments arranged by size:
444 101 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 642
Size: 307 335
BsiEI CG_ry'CG
Cuts at: 0 47 642
Size: 47 595
BsiHKAI G_wGCw'C
Cuts at: 0 53 68 642
Size: 53 15 574
Fragments arranged by size:
574 53 15
Bsp1286I G_dGCh'C
Cuts at: 0 53 68 506 589 642
Size: 53 15 438 83 53
Fragments arranged by size:
438 83 53 53 15
BsrDI GCAATG_nn'
Cuts at: 0 236 642
Size: 236 406
BsrFI r'CCGG_y
Cuts at: 0 228 642
Size: 228 414
BstEII G'GTnAC_C
Cuts at: 0 231 642
Size: 231 411
BstYI r'GATC_y
Cuts at: 0 188 642
Size: 188 454
BtgI C'CryG_G
Cuts at: 0 266 275 642
Size: 266 9 367
Fragments arranged by size:
367 266 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 163 642
Size: 163 479
BtsI GCAGTG_nn'
Cuts at: 0 44 642
Size: 44 598
EaeI y'GGCC_r
Cuts at: 0 348 642
Size: 348 294
EarI CTCTTCn'nnn_
Cuts at: 0 413 419 548 642
Size: 413 6 129 94
Fragments arranged by size:
413 129 94 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 96 642
Size: 96 546
EcoICRI GAG'CTC
Cuts at: 0 66 642
Size: 66 576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 127 232 484 642
Size: 78 49 105 252 158
Fragments arranged by size:
252 158 105 78 49
EcoO109I rG'GnC_Cy
Cuts at: 0 331 642
Size: 331 311
HaeII r_GCGC'y
Cuts at: 0 17 642
Size: 17 625
MscI TGG'CCA
Cuts at: 0 350 642
Size: 350 292
MspA1I CmG'CkG
Cuts at: 0 105 642
Size: 105 537
NcoI C'CATG_G
Cuts at: 0 266 275 642
Size: 266 9 367
Fragments arranged by size:
367 266 9
NsiI A_TGCA'T
Cuts at: 0 179 642
Size: 179 463
PpuMI rG'GwC_Cy
Cuts at: 0 331 642
Size: 331 311
PstI C_TGCA'G
Cuts at: 0 127 642
Size: 127 515
PvuII CAG'CTG
Cuts at: 0 105 642
Size: 105 537
SacI G_AGCT'C
Cuts at: 0 68 642
Size: 68 574
SfcI C'TryA_G
Cuts at: 0 123 642
Size: 123 519
StuI AGG'CCT
Cuts at: 0 253 642
Size: 253 389
StyI C'CwwG_G
Cuts at: 0 266 275 642
Size: 266 9 367
Fragments arranged by size:
367 266 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 273 319 642
Size: 273 46 323
Fragments arranged by size:
323 273 46
Enzymes that do cut:
AclI AcuI AlfI BanI BanII BfrBI Bme1580I BmrI
BpmI Bpu10I BsaI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BsrDI BsrFI BstEII BstYI BtgI BtgZI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII MscI
MspA1I NcoI NsiI PpuMI PstI PvuII SacI SfcI
StuI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI